Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   ACMXYQ_RS01435 Genome accession   NZ_CP180544
Coordinates   322157..323191 (+) Length   344 a.a.
NCBI ID   WP_415893176.1    Uniprot ID   -
Organism   Neptuniibacter sp. PT34_22     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 317157..328191
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACMXYQ_RS01405 (ACMXYQ_01405) - 317362..317811 (-) 450 WP_415895549.1 DUF4426 domain-containing protein -
  ACMXYQ_RS01410 (ACMXYQ_01410) metW 317824..318414 (-) 591 WP_415893171.1 methionine biosynthesis protein MetW -
  ACMXYQ_RS01415 (ACMXYQ_01415) metX 318414..319583 (-) 1170 WP_415895550.1 homoserine O-succinyltransferase MetX -
  ACMXYQ_RS01420 (ACMXYQ_01420) - 319880..320425 (-) 546 WP_415893173.1 YggT family protein -
  ACMXYQ_RS01425 (ACMXYQ_01425) proC 320524..321351 (-) 828 WP_415895551.1 pyrroline-5-carboxylate reductase -
  ACMXYQ_RS01430 (ACMXYQ_01430) - 321370..322065 (-) 696 WP_415895552.1 YggS family pyridoxal phosphate-dependent enzyme -
  ACMXYQ_RS01435 (ACMXYQ_01435) pilT 322157..323191 (+) 1035 WP_415893176.1 type IV pilus twitching motility protein PilT Machinery gene
  ACMXYQ_RS01440 (ACMXYQ_01440) pilU 323214..324380 (+) 1167 WP_415893177.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ACMXYQ_RS01445 (ACMXYQ_01445) - 324457..325731 (-) 1275 WP_415895553.1 dihydroorotase -
  ACMXYQ_RS01450 (ACMXYQ_01450) - 325737..326741 (-) 1005 WP_415895554.1 aspartate carbamoyltransferase catalytic subunit -
  ACMXYQ_RS01455 (ACMXYQ_01455) pyrR 326751..327266 (-) 516 WP_415893180.1 bifunctional pyr operon transcriptional regulator/uracil phosphoribosyltransferase PyrR -
  ACMXYQ_RS01460 (ACMXYQ_01460) ruvX 327247..327723 (-) 477 WP_415893181.1 Holliday junction resolvase RuvX -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 38228.87 Da        Isoelectric Point: 6.5797

>NTDB_id=1096346 ACMXYQ_RS01435 WP_415893176.1 322157..323191(+) (pilT) [Neptuniibacter sp. PT34_22]
MDITELLSFTVQQGASDLHISAGMPPIIRVDGDVRRIKLPSLDHKQVHTLVYDIMNDKQRKEYEDVLETDFSFEVPGLAR
FRVNAFNQNRGAGAVFRTIPSKVLTLDDLDMGKVFQDLSEKPRGLVLVTGPTGSGKSTTLAAMIDYINDTRADHILTIED
PIEFVHESKKCLVNQREVHRDTHSFAHALRSALREDPDIILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRV
IDVFPAAEKDMVRSMLSESLMGVISQTLLKKPKGGRVAAHEIMIGTPAIRNLIREDKVAQMYSAIQTGAAFGMKTLDQSL
QELMQKGLISRETARERAANPQQF

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=1096346 ACMXYQ_RS01435 WP_415893176.1 322157..323191(+) (pilT) [Neptuniibacter sp. PT34_22]
ATGGATATTACCGAACTTTTATCATTTACCGTACAACAAGGCGCATCTGACTTGCATATCTCTGCAGGGATGCCCCCTAT
TATTCGTGTTGACGGTGATGTGCGTCGAATAAAACTGCCTTCATTGGATCATAAGCAGGTCCACACACTCGTATACGACA
TTATGAACGATAAACAACGTAAAGAGTACGAAGATGTGCTTGAAACGGATTTTTCGTTCGAAGTGCCTGGTTTAGCACGT
TTTCGTGTCAATGCATTTAACCAGAACCGTGGTGCCGGTGCTGTTTTCCGTACGATCCCGAGCAAGGTATTGACCCTAGA
TGATCTTGATATGGGTAAGGTTTTTCAGGACTTGTCTGAAAAACCGCGTGGTTTAGTGCTTGTGACGGGGCCAACGGGTT
CCGGTAAAAGTACCACCTTGGCTGCAATGATTGATTACATCAATGATACTCGCGCAGATCACATTCTTACAATCGAAGAT
CCAATCGAATTCGTGCATGAAAGTAAAAAGTGCTTGGTTAACCAGCGTGAAGTGCATCGAGATACTCATAGCTTTGCACA
TGCGCTTCGATCGGCACTTCGTGAAGACCCGGATATTATCCTTGTAGGTGAGATGCGAGACTTGGAAACTATCCGTCTTG
CGCTTACGGCTGCGGAAACAGGTCACTTAGTGTTTGGTACTTTGCATACGACCTCTGCAGCAAAAACAATTGACCGTGTG
ATTGACGTATTTCCAGCGGCTGAGAAAGATATGGTTCGATCCATGCTTTCAGAATCACTGATGGGCGTTATATCTCAAAC
ACTTTTGAAAAAGCCGAAAGGCGGACGTGTTGCAGCTCACGAAATTATGATCGGTACACCAGCTATTCGTAACCTTATTC
GTGAGGATAAAGTGGCGCAGATGTATTCTGCGATCCAGACGGGCGCTGCATTTGGTATGAAAACCTTGGATCAATCTCTA
CAAGAGTTGATGCAGAAAGGACTAATCTCTCGCGAGACAGCGCGTGAGCGAGCAGCAAACCCACAACAGTTCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

82.267

100

0.823
  pilT Pseudomonas stutzeri DSM 10701

81.395

100

0.814
  pilT Acinetobacter nosocomialis M2

81.105

100

0.811
  pilT Acinetobacter baumannii D1279779

81.105

100

0.811
  pilT Acinetobacter baumannii strain A118

81.105

100

0.811
  pilT Acinetobacter baylyi ADP1

79.651

100

0.797
  pilT Legionella pneumophila strain Lp02

73.256

100

0.733
  pilT Legionella pneumophila strain ERS1305867

73.256

100

0.733
  pilT Neisseria meningitidis 8013

67.638

99.709

0.674
  pilT Neisseria gonorrhoeae MS11

67.347

99.709

0.672
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

67.062

97.965

0.657
  pilT Vibrio cholerae strain A1552

67.062

97.965

0.657
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.725

100

0.509
  pilU Pseudomonas stutzeri DSM 10701

41.791

97.384

0.407
  pilU Vibrio cholerae strain A1552

41.493

97.384

0.404
  pilU Acinetobacter baylyi ADP1

39.017

100

0.392

Multiple sequence alignment