Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   ACMXYJ_RS03225 Genome accession   NZ_CP180480
Coordinates   702461..703495 (+) Length   344 a.a.
NCBI ID   WP_056738953.1    Uniprot ID   -
Organism   Pseudomonas sp. LT1P18     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 697461..708495
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACMXYJ_RS03190 - 697541..697975 (-) 435 WP_415758750.1 DUF4426 domain-containing protein -
  ACMXYJ_RS03195 metW 698002..698622 (-) 621 WP_415758751.1 methionine biosynthesis protein MetW -
  ACMXYJ_RS03200 metX 698630..699769 (-) 1140 WP_415758752.1 homoserine O-succinyltransferase MetX -
  ACMXYJ_RS03205 - 699951..700247 (-) 297 WP_415758753.1 DUF167 domain-containing protein -
  ACMXYJ_RS03210 - 700252..700842 (-) 591 WP_415758754.1 YggT family protein -
  ACMXYJ_RS03215 proC 700853..701671 (-) 819 WP_415758755.1 pyrroline-5-carboxylate reductase -
  ACMXYJ_RS03220 - 701707..702405 (-) 699 WP_415758756.1 YggS family pyridoxal phosphate-dependent enzyme -
  ACMXYJ_RS03225 pilT 702461..703495 (+) 1035 WP_056738953.1 type IV pilus twitching motility protein PilT Machinery gene
  ACMXYJ_RS03230 - 703599..704219 (-) 621 WP_415758757.1 C40 family peptidase -
  ACMXYJ_RS03235 - 704470..704889 (+) 420 WP_415758758.1 NINE protein -
  ACMXYJ_RS03240 - 704981..706252 (-) 1272 WP_140675035.1 dihydroorotase -
  ACMXYJ_RS03245 - 706249..707253 (-) 1005 WP_074871640.1 aspartate carbamoyltransferase catalytic subunit -
  ACMXYJ_RS03250 pyrR 707281..707784 (-) 504 WP_008152613.1 bifunctional pyr operon transcriptional regulator/uracil phosphoribosyltransferase PyrR -
  ACMXYJ_RS03255 ruvX 707869..708306 (-) 438 WP_005792259.1 Holliday junction resolvase RuvX -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 37964.63 Da        Isoelectric Point: 7.1852

>NTDB_id=1096059 ACMXYJ_RS03225 WP_056738953.1 702461..703495(+) (pilT) [Pseudomonas sp. LT1P18]
MDITELLAFSAKQGASDLHLSAGLPPMIRVDGDVRRINLPALDHKQVQALIYDIMNDTQRVDFEKHLETDFSFDVPGVAR
FRVNAFNHNRGAGAVFRTIPSKVLSMEDLGMGEVFRKITEAPRGLVLVTGPTGSGKSTTLAAMIDHLNTHRHQHILTIED
PIEFVHESRKCLINQREVHRDTRSFATALRSALREDPDVILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRV
VDVFPGDEKSMVRSMLSESLLAVVSQTLVKKIGGGRIAAHEIMLGTSAIRNLIREDKVAQMYSSIQTGGNLGMQTLDMCL
KELVSKGLISREHAREKARTPDGF

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=1096059 ACMXYJ_RS03225 WP_056738953.1 702461..703495(+) (pilT) [Pseudomonas sp. LT1P18]
ATGGATATCACTGAGCTGCTGGCTTTCAGCGCGAAACAGGGCGCGTCCGACTTGCATCTGTCTGCCGGCCTGCCACCGAT
GATTCGGGTCGACGGCGATGTGCGGCGCATCAACCTGCCAGCCCTGGACCACAAACAGGTGCAGGCGCTGATCTACGACA
TCATGAACGACACGCAACGGGTGGACTTCGAGAAGCACCTGGAGACCGACTTTTCCTTTGACGTGCCCGGCGTGGCGCGT
TTTCGGGTTAACGCATTCAACCACAATCGCGGTGCTGGCGCGGTGTTCCGGACCATTCCGTCCAAGGTCCTGAGCATGGA
AGATCTCGGTATGGGGGAAGTGTTTCGCAAGATTACCGAAGCGCCGCGAGGGCTGGTGCTGGTGACCGGTCCGACCGGTT
CCGGCAAATCCACCACCCTGGCAGCGATGATCGATCACCTGAACACCCATCGCCATCAGCATATTCTCACGATCGAAGAC
CCGATCGAGTTCGTCCACGAGTCGCGCAAATGCTTGATCAATCAGCGTGAAGTCCATCGTGATACGCGCAGCTTCGCCAC
GGCTCTACGCTCGGCCCTGCGGGAGGACCCGGACGTGATTCTGGTGGGGGAGATGCGGGATCTGGAGACCATTCGCCTGG
CGTTGACCGCCGCCGAAACCGGTCATCTGGTGTTTGGCACGCTGCACACGACATCGGCGGCGAAAACCATCGACCGGGTG
GTGGACGTGTTTCCGGGGGACGAGAAATCGATGGTTCGTTCGATGCTGTCCGAGTCGTTACTGGCGGTGGTATCGCAGAC
GTTGGTCAAGAAGATCGGTGGCGGGCGGATTGCGGCCCACGAAATCATGCTCGGCACGTCAGCGATCCGTAACCTGATCC
GCGAAGACAAGGTGGCGCAGATGTACTCGTCGATTCAGACCGGGGGAAACCTGGGGATGCAGACGCTGGATATGTGCTTG
AAGGAGCTGGTGAGCAAGGGCTTGATCAGTCGCGAGCATGCGCGGGAGAAGGCGCGCACGCCGGATGGGTTTTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas stutzeri DSM 10701

86.337

100

0.863
  pilT Pseudomonas aeruginosa PAK

85.465

100

0.855
  pilT Acinetobacter nosocomialis M2

78.488

100

0.785
  pilT Acinetobacter baumannii D1279779

78.198

100

0.782
  pilT Acinetobacter baumannii strain A118

78.198

100

0.782
  pilT Acinetobacter baylyi ADP1

76.453

100

0.765
  pilT Legionella pneumophila strain Lp02

71.221

100

0.712
  pilT Legionella pneumophila strain ERS1305867

71.221

100

0.712
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

67.359

97.965

0.66
  pilT Vibrio cholerae strain A1552

67.359

97.965

0.66
  pilT Neisseria meningitidis 8013

65.797

100

0.66
  pilT Neisseria gonorrhoeae MS11

65.507

100

0.657
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.594

100

0.517
  pilU Vibrio cholerae strain A1552

39.942

99.709

0.398
  pilU Acinetobacter baylyi ADP1

39.595

100

0.398
  pilU Pseudomonas stutzeri DSM 10701

40.597

97.384

0.395

Multiple sequence alignment