Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   ACLHDG_RS11500 Genome accession   NZ_CP180410
Coordinates   2350129..2351196 (+) Length   355 a.a.
NCBI ID   WP_415406379.1    Uniprot ID   -
Organism   Sulfurovum sp. CS9     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2345129..2356196
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACLHDG_RS11460 (ACLHDG_11455) - 2345722..2346744 (-) 1023 WP_415406371.1 sensor histidine kinase -
  ACLHDG_RS11465 (ACLHDG_11460) - 2346747..2346953 (-) 207 WP_415406372.1 hypothetical protein -
  ACLHDG_RS11470 (ACLHDG_11465) - 2347037..2347462 (-) 426 WP_415406373.1 thioredoxin family protein -
  ACLHDG_RS11475 (ACLHDG_11470) dapE 2347472..2348572 (-) 1101 WP_415406374.1 succinyl-diaminopimelate desuccinylase -
  ACLHDG_RS11480 (ACLHDG_11475) - 2348576..2348920 (-) 345 WP_415406375.1 rhodanese-like domain-containing protein -
  ACLHDG_RS11485 (ACLHDG_11480) - 2348975..2349388 (-) 414 WP_415406376.1 ferredoxin-thioredoxin reductase catalytic domain-containing protein -
  ACLHDG_RS11490 (ACLHDG_11485) gatC 2349470..2349760 (+) 291 WP_415406377.1 Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase subunit GatC -
  ACLHDG_RS11495 (ACLHDG_11490) - 2349772..2350119 (+) 348 WP_415406378.1 arsenate reductase family protein -
  ACLHDG_RS11500 (ACLHDG_11495) pilT 2350129..2351196 (+) 1068 WP_415406379.1 type IV pilus twitching motility protein PilT Machinery gene
  ACLHDG_RS11505 (ACLHDG_11500) - 2351261..2351902 (+) 642 WP_415406380.1 CvpA family protein -
  ACLHDG_RS11510 (ACLHDG_11505) - 2351912..2352361 (+) 450 WP_415406381.1 Fur family transcriptional regulator -
  ACLHDG_RS11515 (ACLHDG_11510) lysS 2352552..2354201 (+) 1650 WP_415406382.1 lysine--tRNA ligase -
  ACLHDG_RS11520 (ACLHDG_11515) - 2354252..2355502 (+) 1251 WP_415406383.1 serine hydroxymethyltransferase -
  ACLHDG_RS11525 (ACLHDG_11520) - 2355511..2355693 (+) 183 Protein_2267 DUF1882 domain-containing protein -
  ACLHDG_RS11530 (ACLHDG_11525) - 2355724..2356005 (+) 282 WP_415406384.1 hypothetical protein -

Sequence

Protein


Download         Length: 355 a.a.        Molecular weight: 39085.82 Da        Isoelectric Point: 6.2856

>NTDB_id=1095751 ACLHDG_RS11500 WP_415406379.1 2350129..2351196(+) (pilT) [Sulfurovum sp. CS9]
MAAFDIKKLLQSVVSHGASDLHLVSRTEPQIRLDGALKPVNLPKLTGEDIEEMCYSLITEKQKQHFEEYDELDFALLIPG
IGRFRANYYRTLGDTAAAFRTIPIDIPSLDDLGAPEIYKKLMQREKGLILVTGPTGSGKSTTLAAMLNEINLYEHKHVVT
VEDPVEFIHQNKKCVFSHRGVGEDTKSFATALKFAMRQDPDIILIGEMRDKETIEAALTAAETGHLVFGTLHTSSAPGTI
NRIIDVFSGDEQPQIRAMISSSLVAIIAQALLPKLGGGRVAASEILITNHAISNLIREDKVHQIYSQMQLGQGDTGMQTQ
TQALLKFLKDGKISRDIALQYANKADELSKATQAY

Nucleotide


Download         Length: 1068 bp        

>NTDB_id=1095751 ACLHDG_RS11500 WP_415406379.1 2350129..2351196(+) (pilT) [Sulfurovum sp. CS9]
ATGGCTGCTTTCGATATAAAAAAACTACTTCAGAGTGTTGTTTCACATGGTGCTTCTGACTTGCACCTTGTGAGTCGCAC
AGAACCACAGATCAGACTGGATGGTGCACTCAAACCTGTAAACCTTCCTAAGCTTACGGGTGAAGATATTGAAGAGATGT
GCTACTCTTTGATCACTGAAAAACAAAAACAACATTTCGAAGAATACGATGAACTTGACTTTGCACTTTTAATTCCCGGC
ATTGGTCGTTTTAGAGCAAACTATTATCGTACTTTAGGTGATACTGCCGCCGCATTTAGAACCATTCCTATAGATATACC
TTCCTTGGACGACCTTGGCGCACCGGAAATCTATAAAAAACTTATGCAAAGAGAAAAAGGTCTCATTCTGGTAACCGGTC
CAACCGGTTCTGGTAAATCAACTACTCTTGCTGCTATGCTGAATGAAATCAACCTGTATGAGCACAAACACGTTGTTACA
GTAGAAGATCCGGTGGAATTTATACACCAAAATAAAAAATGTGTTTTCTCACACAGAGGTGTCGGTGAAGATACAAAAAG
TTTTGCCACAGCACTCAAGTTTGCAATGCGTCAGGATCCAGACATTATCCTCATTGGGGAGATGAGGGATAAAGAGACCA
TAGAAGCTGCACTCACAGCAGCGGAAACCGGGCACCTTGTTTTTGGTACACTTCACACCTCTTCTGCACCTGGTACGATC
AACCGTATTATTGATGTATTCTCCGGAGATGAACAACCGCAGATCAGAGCCATGATCTCATCATCACTTGTGGCAATTAT
CGCTCAGGCACTTCTGCCTAAACTTGGTGGTGGACGTGTAGCTGCTTCAGAGATCTTGATCACAAATCATGCGATCTCCA
ACCTTATCCGTGAAGATAAAGTACACCAGATCTATTCACAAATGCAATTGGGTCAAGGTGATACAGGTATGCAAACGCAG
ACACAGGCACTCTTGAAGTTCCTTAAAGATGGAAAGATCTCTCGTGATATTGCTTTACAGTATGCCAACAAAGCGGATGA
ACTATCAAAAGCAACACAAGCTTACTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(9-274)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas stutzeri DSM 10701

51.304

97.183

0.499
  pilT Legionella pneumophila strain Lp02

51.603

96.62

0.499
  pilT Legionella pneumophila strain ERS1305867

51.603

96.62

0.499
  pilT Acinetobacter nosocomialis M2

51.014

97.183

0.496
  pilT Acinetobacter baumannii D1279779

51.014

97.183

0.496
  pilT Acinetobacter baumannii strain A118

51.014

97.183

0.496
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

52.121

92.958

0.485
  pilT Vibrio cholerae strain A1552

52.121

92.958

0.485
  pilT Pseudomonas aeruginosa PAK

50.737

95.493

0.485
  pilT Acinetobacter baylyi ADP1

51.36

93.239

0.479
  pilT Neisseria gonorrhoeae MS11

48.127

97.746

0.47
  pilT Neisseria meningitidis 8013

47.839

97.746

0.468
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.286

98.592

0.437
  pilU Acinetobacter baylyi ADP1

40.634

97.746

0.397
  pilU Pseudomonas stutzeri DSM 10701

39.884

97.465

0.389
  pilU Vibrio cholerae strain A1552

37.572

97.465

0.366

Multiple sequence alignment