Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   ACLM42_RS11830 Genome accession   NZ_CP180404
Coordinates   2157695..2158768 (-) Length   357 a.a.
NCBI ID   WP_247909800.1    Uniprot ID   -
Organism   Synechococcus sp. W4D4     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2152695..2163768
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACLM42_RS11810 (ACLM42_11810) - 2153640..2154191 (+) 552 WP_247909796.1 methyltransferase family protein -
  ACLM42_RS11815 (ACLM42_11815) dusA 2154193..2155191 (-) 999 WP_247909797.1 tRNA dihydrouridine(20/20a) synthase DusA -
  ACLM42_RS11820 (ACLM42_11820) - 2155217..2156467 (+) 1251 WP_415399229.1 NAD(P)/FAD-dependent oxidoreductase -
  ACLM42_RS11825 (ACLM42_11825) - 2156460..2157695 (-) 1236 WP_247909799.1 type II secretion system F family protein -
  ACLM42_RS11830 (ACLM42_11830) pilT 2157695..2158768 (-) 1074 WP_247909800.1 type IV pilus twitching motility protein PilT Machinery gene
  ACLM42_RS11835 (ACLM42_11835) - 2158835..2160655 (-) 1821 WP_415399230.1 GspE/PulE family protein -
  ACLM42_RS11840 (ACLM42_11840) grpE 2160751..2161425 (+) 675 WP_247909802.1 nucleotide exchange factor GrpE -
  ACLM42_RS11845 (ACLM42_11845) dnaJ 2161428..2162555 (+) 1128 WP_415399231.1 molecular chaperone DnaJ -
  ACLM42_RS11850 (ACLM42_11850) - 2162563..2162784 (+) 222 WP_247909804.1 sulfurtransferase TusA family protein -

Sequence

Protein


Download         Length: 357 a.a.        Molecular weight: 39298.86 Da        Isoelectric Point: 6.2563

>NTDB_id=1095699 ACLM42_RS11830 WP_247909800.1 2157695..2158768(-) (pilT) [Synechococcus sp. W4D4]
MELQIEQLMEELVEAGGSDLHLAAGQPPYGRFSGVLQPMREERLSEDLCNRLIFSMINNAQRKQLEQSWELDCAYGLKGV
SRFRVNVYRQRGSYAACLRALGSTIPSLDSLGLPPIVEETSRRPRGLVLVTGPTGSGKTTTLAALLDHINRSRAEHILTI
EDPIEFTYRNDKSVIHQRQLGEDTRSFAAALRAALREDPDVILVGEMRDLETIQLAITASETGHLVFGTLHTSSAAQTVD
RMVDVFPPEQQTQIRVQLSGSLVGVFSQTLCKRQNPKPGQFGRVMAQEILINTPAIANLIREGKTAQLYSQIQTGGQQGM
QTLERALANLVLAGAVERSEALAKASKPDELMRLLEG

Nucleotide


Download         Length: 1074 bp        

>NTDB_id=1095699 ACLM42_RS11830 WP_247909800.1 2157695..2158768(-) (pilT) [Synechococcus sp. W4D4]
ATGGAGCTCCAGATCGAACAGTTGATGGAGGAGCTGGTCGAGGCAGGTGGCAGTGATCTGCACCTGGCCGCGGGCCAACC
GCCCTACGGCCGCTTCAGTGGCGTGCTCCAACCGATGCGCGAGGAGCGCCTCAGCGAGGACCTCTGCAACCGGTTGATCT
TCTCGATGATCAACAACGCCCAGCGCAAGCAGCTGGAGCAAAGCTGGGAGCTGGACTGCGCCTATGGCCTCAAAGGCGTC
TCGCGCTTCCGGGTCAATGTCTACCGCCAACGGGGCAGCTACGCCGCTTGCCTGCGGGCCCTTGGCAGCACGATTCCCAG
CTTGGACAGCCTGGGACTCCCCCCCATCGTCGAAGAGACCAGCCGTAGGCCCAGGGGTCTTGTGCTGGTGACCGGCCCCA
CAGGCTCCGGCAAAACCACAACCCTGGCGGCCCTGCTGGATCACATCAACCGCAGCCGCGCCGAGCACATCCTCACGATC
GAAGACCCGATCGAATTCACCTACCGCAACGACAAAAGCGTGATCCACCAGCGCCAGCTGGGGGAAGACACCCGCAGCTT
CGCCGCCGCCCTGCGAGCGGCCCTAAGGGAGGACCCGGACGTGATCCTCGTCGGCGAGATGCGGGACCTCGAAACGATTC
AGCTGGCGATCACGGCCTCAGAGACCGGGCATCTGGTCTTCGGCACCCTGCACACCAGCTCGGCGGCGCAAACCGTGGAT
CGCATGGTCGATGTCTTCCCACCGGAGCAGCAAACCCAAATCCGTGTTCAGCTGAGCGGCTCTCTGGTGGGGGTGTTCTC
CCAAACCCTGTGCAAACGCCAGAACCCCAAACCCGGTCAATTCGGGCGGGTCATGGCCCAGGAAATCTTGATCAACACCC
CGGCGATCGCCAACTTGATCCGGGAGGGCAAAACGGCCCAGCTCTATTCCCAGATCCAAACCGGCGGTCAGCAGGGCATG
CAAACCCTGGAGCGCGCCCTCGCCAACCTGGTGCTGGCCGGAGCCGTTGAGCGCAGTGAAGCCCTCGCCAAGGCGAGCAA
GCCAGACGAGCTGATGCGGCTCCTGGAGGGCTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(82-246)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

51.289

97.759

0.501
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.284

98.599

0.496
  pilT Pseudomonas stutzeri DSM 10701

50.143

97.759

0.49
  pilT Acinetobacter baylyi ADP1

50.143

97.759

0.49
  pilT Acinetobacter baumannii strain A118

49.714

98.039

0.487
  pilT Acinetobacter baumannii D1279779

49.714

98.039

0.487
  pilT Acinetobacter nosocomialis M2

49.714

98.039

0.487
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50.292

95.798

0.482
  pilT Vibrio cholerae strain A1552

50.292

95.798

0.482
  pilT Legionella pneumophila strain ERS1305867

50.737

94.958

0.482
  pilT Legionella pneumophila strain Lp02

50.737

94.958

0.482
  pilT Neisseria gonorrhoeae MS11

48.138

97.759

0.471
  pilT Neisseria meningitidis 8013

50

93.557

0.468
  pilU Pseudomonas stutzeri DSM 10701

37.536

97.759

0.367
  pilU Vibrio cholerae strain A1552

36.723

99.16

0.364
  pilU Acinetobacter baylyi ADP1

36.648

98.599

0.361

Multiple sequence alignment