Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   ACMGRF_RS00745 Genome accession   NZ_CP179923
Coordinates   161631..162758 (-) Length   375 a.a.
NCBI ID   WP_014035879.1    Uniprot ID   A0AAJ1X8S1
Organism   Stenotrophomonas sp. EMP41     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 156631..167758
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACMGRF_RS00725 (ACMGRF_00725) - 157059..158048 (+) 990 WP_099486317.1 fumarylacetoacetate hydrolase family protein -
  ACMGRF_RS00730 (ACMGRF_00730) maiA 158063..158731 (+) 669 WP_049414451.1 maleylacetoacetate isomerase -
  ACMGRF_RS00735 (ACMGRF_00735) - 158775..160235 (-) 1461 WP_414485551.1 UdgX family uracil-DNA binding protein -
  ACMGRF_RS00740 (ACMGRF_00740) - 160216..161499 (-) 1284 WP_049414453.1 putative DNA modification/repair radical SAM protein -
  ACMGRF_RS00745 (ACMGRF_00745) pilU 161631..162758 (-) 1128 WP_014035879.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ACMGRF_RS00750 (ACMGRF_00750) - 162880..163245 (+) 366 WP_005415160.1 DUF4398 domain-containing protein -
  ACMGRF_RS00755 (ACMGRF_00755) - 163242..164126 (+) 885 WP_414485552.1 hypothetical protein -
  ACMGRF_RS00760 (ACMGRF_00760) - 164451..164687 (+) 237 WP_005407964.1 YdcH family protein -
  ACMGRF_RS00765 (ACMGRF_00765) - 164989..166359 (+) 1371 WP_049416902.1 pyridoxal-phosphate dependent enzyme -
  ACMGRF_RS00770 (ACMGRF_00770) - 166483..167667 (+) 1185 WP_099486310.1 cystathionine gamma-synthase -

Sequence

Protein


Download         Length: 375 a.a.        Molecular weight: 41410.75 Da        Isoelectric Point: 6.3741

>NTDB_id=1093853 ACMGRF_RS00745 WP_014035879.1 161631..162758(-) (pilU) [Stenotrophomonas sp. EMP41]
MDIGYFLKLMTEKNASDMFLTTGAPVYIKIEGKLYPLGNTGLPPGMVKKIAYSLMDEGQVPQFERELELNMAIALPDAGR
FRVNVFKQRGEVGMVIRAIRSRIPSIEELNLPQVLKDVIMTPRGLVLVVGSTGSGKSTSLASMIDHRNSTTTGHILTIED
PIEYLHKHKMSIVNQREVGLDTHAFHNALKNAMREAPDVILIGEILDAETMEAAIAFAETGHLCLATLHSNNADQTIERI
LNFFPESAHKNVLMNLALNLRAVISQRLVKNKEGRRLPATEVLINTPMIRDLLRRGQVHEIKAAMEASLEEGMESFDQCL
FRLAKAGIIEQEEALRAADSRDGLALKFRLSEGSSGEHDPYADFSAGAPSITHGF

Nucleotide


Download         Length: 1128 bp        

>NTDB_id=1093853 ACMGRF_RS00745 WP_014035879.1 161631..162758(-) (pilU) [Stenotrophomonas sp. EMP41]
ATGGATATCGGCTACTTCCTGAAGCTGATGACCGAAAAGAACGCTTCGGACATGTTTCTGACCACCGGGGCACCGGTCTA
CATCAAGATCGAGGGGAAGCTCTATCCGCTGGGCAACACCGGCCTGCCGCCGGGCATGGTCAAGAAGATCGCCTACTCGC
TGATGGATGAGGGCCAGGTGCCGCAGTTCGAGCGCGAGCTCGAGCTCAACATGGCCATCGCCCTGCCCGATGCCGGGCGC
TTCCGCGTCAACGTGTTCAAGCAGCGGGGCGAGGTCGGCATGGTCATCCGCGCCATCCGCAGCCGCATCCCGAGCATCGA
GGAGCTGAACCTGCCGCAGGTGCTGAAGGACGTCATCATGACCCCGCGCGGGCTGGTGCTGGTGGTGGGGTCCACCGGCT
CCGGCAAGTCGACCTCGCTGGCGTCGATGATCGACCACCGCAACAGCACCACCACCGGTCACATCCTCACCATCGAGGAT
CCGATCGAGTACCTGCACAAGCACAAGATGTCGATCGTCAACCAGCGCGAGGTCGGCCTGGACACCCATGCCTTCCACAA
CGCGCTGAAGAACGCGATGCGTGAAGCGCCGGACGTGATCCTGATCGGCGAGATCCTGGATGCGGAGACGATGGAGGCGG
CGATCGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCCACCCTGCACTCCAACAACGCCGACCAGACCATCGAGCGCATC
CTCAACTTCTTCCCGGAAAGCGCGCACAAGAACGTGCTGATGAACCTGGCGCTGAACCTGCGCGCGGTGATCAGCCAGCG
CCTGGTGAAGAACAAGGAGGGCCGACGCCTGCCGGCCACCGAGGTGCTGATCAACACGCCGATGATCCGCGACCTGCTGC
GTCGCGGCCAGGTGCACGAGATCAAGGCGGCGATGGAAGCCTCGCTGGAGGAAGGCATGGAGAGCTTTGACCAGTGCCTG
TTCCGGCTGGCCAAGGCGGGCATCATCGAGCAGGAGGAAGCGCTGCGCGCAGCCGACTCGCGCGATGGCCTGGCCCTGAA
GTTCCGCCTTTCCGAGGGCAGCAGCGGCGAGCACGACCCGTACGCCGATTTCTCGGCGGGCGCGCCCAGCATCACCCACG
GCTTCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(81-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

51.253

95.733

0.491
  pilU Acinetobacter baylyi ADP1

49.435

94.4

0.467
  pilU Vibrio cholerae strain A1552

49.153

94.4

0.464
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.772

99.467

0.445
  pilT Pseudomonas aeruginosa PAK

42.899

90.133

0.387
  pilT Acinetobacter baylyi ADP1

41.765

90.667

0.379
  pilT Acinetobacter baumannii strain A118

41.716

90.133

0.376
  pilT Acinetobacter nosocomialis M2

41.716

90.133

0.376
  pilT Acinetobacter baumannii D1279779

41.716

90.133

0.376
  pilT Pseudomonas stutzeri DSM 10701

41.42

90.133

0.373
  pilT Legionella pneumophila strain Lp02

40.828

90.133

0.368
  pilT Legionella pneumophila strain ERS1305867

40.828

90.133

0.368
  pilT Vibrio cholerae strain A1552

40.841

88.8

0.363
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

40.841

88.8

0.363
  pilT Neisseria meningitidis 8013

39.193

92.533

0.363
  pilT Neisseria gonorrhoeae MS11

39.193

92.533

0.363

Multiple sequence alignment