Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   ACLSSQ_RS01395 Genome accession   NZ_CP179682
Coordinates   276768..277811 (-) Length   347 a.a.
NCBI ID   WP_014235715.1    Uniprot ID   A0A5H2YC97
Organism   Azospira sp. APE16     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 271768..282811
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACLSSQ_RS01370 (ACLSSQ_01370) - 271965..272978 (-) 1014 WP_287875390.1 CDP-6-deoxy-delta-3,4-glucoseen reductase -
  ACLSSQ_RS01375 (ACLSSQ_01375) - 273029..273934 (+) 906 WP_416243579.1 NAD-dependent epimerase/dehydratase family protein -
  ACLSSQ_RS01380 (ACLSSQ_01380) - 273928..274344 (+) 417 WP_416243580.1 CopD family protein -
  ACLSSQ_RS01385 (ACLSSQ_01385) - 274356..275528 (+) 1173 WP_416243581.1 THUMP domain-containing class I SAM-dependent RNA methyltransferase -
  ACLSSQ_RS01390 (ACLSSQ_01390) - 275616..276751 (-) 1136 Protein_277 PilT/PilU family type 4a pilus ATPase -
  ACLSSQ_RS01395 (ACLSSQ_01395) pilT 276768..277811 (-) 1044 WP_014235715.1 type IV pilus twitching motility protein PilT Machinery gene
  ACLSSQ_RS01400 (ACLSSQ_01400) - 277970..278674 (+) 705 WP_416243582.1 YggS family pyridoxal phosphate-dependent enzyme -
  ACLSSQ_RS01405 (ACLSSQ_01405) proC 278688..279500 (+) 813 WP_014235713.1 pyrroline-5-carboxylate reductase -
  ACLSSQ_RS01410 (ACLSSQ_01410) - 279540..280112 (+) 573 WP_130459761.1 YggT family protein -
  ACLSSQ_RS01415 (ACLSSQ_01415) - 280112..280402 (+) 291 WP_152090054.1 DUF167 domain-containing protein -
  ACLSSQ_RS01420 (ACLSSQ_01420) - 280442..281155 (-) 714 WP_416243583.1 LytTR family transcriptional regulator DNA-binding domain-containing protein -
  ACLSSQ_RS01425 (ACLSSQ_01425) cowN 281292..281594 (+) 303 WP_416243584.1 N(2)-fixation sustaining protein CowN -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38391.15 Da        Isoelectric Point: 6.9357

>NTDB_id=1092551 ACLSSQ_RS01395 WP_014235715.1 276768..277811(-) (pilT) [Azospira sp. APE16]
MDITELLAFGVKNKASDLHLSAGLPPMIRVHGDVRRINLPAMEHKDVHAMVYDIMNDGQRKTYEENLECDFSFEVPNLAR
FRVNAFVQHRGAGAVFRTIPSKVLSLEDLNAPKIFKEISEFPRGIVLVTGPTGSGKSTTLAAMVNHINENDYGHILTVED
PIEFVHEAKKCLINQREVGPHTLSFNNALRSALREDPDVILVGEMRDLETIRLAMTAAETGHLVFGTLHTSSAAKTVDRI
IDVFPAAEKEMIRAMLSESLRAVISQTLLKTKDGNGRVAAHEIMIGTPAIRNLIREAKIAQMYSAIQTGQQFGMQTLDQN
LTDLVRRNVISPAEARTKAVNKDMFGG

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=1092551 ACLSSQ_RS01395 WP_014235715.1 276768..277811(-) (pilT) [Azospira sp. APE16]
ATGGACATCACCGAACTGCTCGCCTTCGGCGTCAAGAACAAGGCCTCCGACCTGCACCTCTCCGCCGGCCTGCCGCCCAT
GATCCGGGTCCACGGCGACGTGCGCCGCATCAACCTGCCGGCCATGGAGCACAAGGACGTGCACGCCATGGTGTACGACA
TCATGAACGACGGCCAGCGCAAGACCTACGAAGAGAACCTGGAGTGCGACTTCTCCTTCGAAGTGCCCAACCTGGCCCGT
TTCCGGGTCAATGCTTTCGTCCAGCACCGGGGCGCCGGCGCCGTCTTCCGGACCATTCCCTCCAAGGTGCTGAGCCTGGA
GGACCTCAACGCGCCGAAGATCTTCAAGGAGATTTCCGAATTCCCCCGGGGCATCGTGCTGGTCACCGGCCCCACCGGTT
CCGGTAAGTCCACCACCCTGGCGGCCATGGTGAACCACATCAACGAAAACGATTACGGCCACATCCTGACCGTGGAAGAC
CCCATCGAATTCGTGCACGAAGCCAAGAAGTGCCTCATCAACCAGCGGGAAGTGGGTCCCCACACCCTGTCCTTCAACAA
CGCCCTGCGCTCGGCCCTGCGGGAAGACCCGGACGTGATCCTGGTGGGCGAAATGCGCGACCTGGAAACCATCCGCCTGG
CCATGACGGCGGCAGAAACGGGTCACCTGGTGTTCGGCACCCTGCACACCTCCTCCGCCGCCAAGACGGTGGACCGGATC
ATCGACGTCTTCCCCGCGGCGGAGAAGGAAATGATCCGGGCCATGCTCTCCGAATCCCTGCGCGCCGTCATTTCCCAGAC
CCTGCTGAAGACCAAGGACGGCAATGGCCGGGTGGCGGCCCACGAGATCATGATCGGCACCCCGGCCATCCGCAACCTGA
TCCGGGAAGCCAAGATCGCCCAGATGTATTCGGCCATCCAGACTGGCCAGCAGTTCGGCATGCAGACCCTGGACCAGAAC
CTCACCGATCTGGTGCGGCGCAACGTCATTTCGCCGGCGGAAGCCCGCACCAAGGCGGTCAACAAGGACATGTTCGGCGG
CTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A5H2YC97

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

79.42

99.424

0.79
  pilT Acinetobacter baumannii D1279779

79.42

99.424

0.79
  pilT Acinetobacter baumannii strain A118

79.42

99.424

0.79
  pilT Acinetobacter baylyi ADP1

75.652

99.424

0.752
  pilT Pseudomonas stutzeri DSM 10701

74.493

99.424

0.741
  pilT Pseudomonas aeruginosa PAK

75.811

97.695

0.741
  pilT Legionella pneumophila strain Lp02

73.913

99.424

0.735
  pilT Legionella pneumophila strain ERS1305867

73.913

99.424

0.735
  pilT Neisseria meningitidis 8013

73.178

98.847

0.723
  pilT Neisseria gonorrhoeae MS11

72.886

98.847

0.72
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

68.343

97.406

0.666
  pilT Vibrio cholerae strain A1552

68.343

97.406

0.666
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.014

99.424

0.507
  pilU Acinetobacter baylyi ADP1

43.155

96.83

0.418
  pilU Vibrio cholerae strain A1552

41.791

96.542

0.403
  pilU Pseudomonas stutzeri DSM 10701

41.493

96.542

0.401

Multiple sequence alignment