Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   ACKW6E_RS02925 Genome accession   NZ_CP178915
Coordinates   660994..662127 (-) Length   377 a.a.
NCBI ID   WP_056930862.1    Uniprot ID   -
Organism   Stenotrophomonas acidaminiphila strain ZCMU-Sa01     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 655994..667127
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACKW6E_RS02900 (ACKW6E_02900) - 656299..656667 (-) 369 WP_413975664.1 phasin family protein -
  ACKW6E_RS02905 (ACKW6E_02905) queD 656760..657116 (-) 357 WP_054662649.1 6-carboxytetrahydropterin synthase QueD -
  ACKW6E_RS02910 (ACKW6E_02910) - 657227..658636 (-) 1410 WP_054662653.1 DEAD/DEAH box helicase -
  ACKW6E_RS02915 (ACKW6E_02915) - 659118..660107 (+) 990 WP_054662656.1 fumarylacetoacetate hydrolase family protein -
  ACKW6E_RS02920 (ACKW6E_02920) maiA 660253..660909 (+) 657 WP_056930910.1 maleylacetoacetate isomerase -
  ACKW6E_RS02925 (ACKW6E_02925) pilU 660994..662127 (-) 1134 WP_056930862.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ACKW6E_RS02930 (ACKW6E_02930) - 662221..662607 (+) 387 WP_056930863.1 DUF4398 domain-containing protein -
  ACKW6E_RS02935 (ACKW6E_02935) - 662607..663488 (+) 882 WP_226050738.1 OmpA family protein -
  ACKW6E_RS02940 (ACKW6E_02940) - 663882..664100 (+) 219 WP_056930865.1 YdcH family protein -
  ACKW6E_RS02945 (ACKW6E_02945) - 664287..665657 (+) 1371 WP_056930866.1 pyridoxal-phosphate dependent enzyme -
  ACKW6E_RS02950 (ACKW6E_02950) - 665728..666915 (+) 1188 WP_413975665.1 cystathionine gamma-synthase -

Sequence

Protein


Download         Length: 377 a.a.        Molecular weight: 41660.92 Da        Isoelectric Point: 6.5062

>NTDB_id=1092430 ACKW6E_RS02925 WP_056930862.1 660994..662127(-) (pilU) [Stenotrophomonas acidaminiphila strain ZCMU-Sa01]
MDIGYFLKLMTEKNASDMFLTTGAPVHIKIEGKLYPLGNTGLPPGMVKKIAYSLMDEGQVPQFERDLELNMAIALADVGR
FRVNVFKQRGEVGMVIRAIKNRIPSIEELKLPDVLKDVIMTPRGLVLVVGSTGSGKSTSLASMIDHRNATTTGHILTIED
PIEFLHRHKQSIVNQREVGLDTHAFHNALKNAMREAPDVILIGEILDAETMEAAIAFAETGHLCLATLHSNNADQTIERI
LNFFPESAHRNVLMNLSLNLRAVISQRLVKGTDGRRRPATEVLINTPMIRDLLRRGQVHEIKQAMEQSLEEGMHSFDQCL
FRLAKEGVIEQEEALRAADSRDGLALKFRLSEGASGEHDPYADFSAAAAPASITHGF

Nucleotide


Download         Length: 1134 bp        

>NTDB_id=1092430 ACKW6E_RS02925 WP_056930862.1 660994..662127(-) (pilU) [Stenotrophomonas acidaminiphila strain ZCMU-Sa01]
ATGGATATCGGCTATTTCCTGAAGCTGATGACCGAAAAAAACGCGTCGGACATGTTCCTGACGACGGGTGCGCCGGTCCA
CATCAAGATCGAAGGAAAGCTGTACCCCCTGGGCAATACCGGCCTGCCGCCGGGCATGGTCAAGAAGATCGCCTACTCGC
TGATGGACGAAGGCCAGGTGCCGCAGTTCGAGCGCGACCTGGAGCTGAACATGGCCATCGCGCTGGCCGACGTGGGCCGG
TTCCGCGTGAACGTGTTCAAGCAGCGCGGCGAAGTGGGCATGGTCATCCGCGCGATCAAGAACCGGATTCCCTCGATCGA
GGAACTGAAGCTGCCGGACGTGCTCAAGGACGTGATCATGACCCCGCGTGGGCTGGTGCTGGTGGTCGGCTCCACCGGCT
CGGGCAAGTCCACGTCGCTGGCGTCGATGATCGACCACCGCAACGCCACCACCACCGGGCACATCCTCACCATCGAGGAC
CCGATCGAATTCCTGCACCGGCACAAGCAGTCCATCGTCAACCAGCGCGAAGTCGGGCTTGATACCCATGCCTTCCACAA
CGCACTGAAGAACGCGATGCGCGAGGCGCCGGACGTGATCCTGATCGGCGAAATCCTCGACGCCGAGACCATGGAAGCGG
CCATCGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCCACGCTGCACTCCAACAACGCCGACCAGACCATCGAGCGCATC
CTCAACTTCTTCCCCGAGAGCGCGCACCGCAACGTGCTGATGAACCTGTCGCTGAACCTGCGCGCGGTGATCAGCCAGCG
CCTGGTCAAGGGCACCGACGGCCGCCGCCGGCCCGCGACCGAGGTGCTGATCAACACGCCGATGATCCGCGACCTGCTGC
GCCGCGGGCAGGTGCACGAGATCAAGCAGGCCATGGAACAGTCGCTGGAGGAGGGCATGCACAGTTTCGACCAGTGCCTG
TTCCGGCTGGCCAAGGAGGGCGTGATCGAGCAGGAGGAGGCGCTGCGCGCGGCCGATTCGCGCGACGGCCTGGCGCTCAA
GTTCCGGCTGTCCGAAGGCGCCAGCGGCGAGCACGACCCCTACGCGGATTTCTCCGCGGCGGCGGCACCGGCGTCGATCA
CCCACGGGTTCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(80-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

52.925

95.225

0.504
  pilU Acinetobacter baylyi ADP1

50.42

94.695

0.477
  pilU Vibrio cholerae strain A1552

49.435

93.899

0.464
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

45.161

98.674

0.446
  pilT Pseudomonas aeruginosa PAK

43.195

89.655

0.387
  pilT Acinetobacter baumannii strain A118

42.012

89.655

0.377
  pilT Acinetobacter nosocomialis M2

42.012

89.655

0.377
  pilT Acinetobacter baumannii D1279779

42.012

89.655

0.377
  pilT Acinetobacter baylyi ADP1

41.471

90.186

0.374
  pilT Pseudomonas stutzeri DSM 10701

41.716

89.655

0.374
  pilT Neisseria meningitidis 8013

40.346

92.042

0.371
  pilT Neisseria gonorrhoeae MS11

40.346

92.042

0.371
  pilT Vibrio cholerae strain A1552

42.042

88.329

0.371
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

42.042

88.329

0.371
  pilT Legionella pneumophila strain ERS1305867

40.828

89.655

0.366
  pilT Legionella pneumophila strain Lp02

40.828

89.655

0.366

Multiple sequence alignment