Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   ACLI4M_RS05530 Genome accession   NZ_CP178576
Coordinates   1020618..1021616 (-) Length   332 a.a.
NCBI ID   WP_133622798.1    Uniprot ID   -
Organism   Erwinia sp. LJJL01     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1015618..1026616
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACLI4M_RS05500 (ACLI4M_05500) - 1016341..1016853 (+) 513 WP_133622802.1 SprT family zinc-dependent metalloprotease -
  ACLI4M_RS05505 (ACLI4M_05505) endA 1016975..1017688 (+) 714 WP_133622801.1 deoxyribonuclease I -
  ACLI4M_RS05510 (ACLI4M_05510) rsmE 1017749..1018480 (+) 732 WP_133622800.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  ACLI4M_RS05515 (ACLI4M_05515) gshB 1018518..1019462 (+) 945 WP_048917246.1 glutathione synthase -
  ACLI4M_RS05520 (ACLI4M_05520) - 1019553..1020116 (+) 564 WP_048917247.1 YqgE/AlgH family protein -
  ACLI4M_RS05525 (ACLI4M_05525) ruvX 1020116..1020538 (+) 423 WP_208864869.1 Holliday junction resolvase RuvX -
  ACLI4M_RS05530 (ACLI4M_05530) pilT 1020618..1021616 (-) 999 WP_133622798.1 type IV pilus twitching motility protein PilT Machinery gene
  ACLI4M_RS05535 (ACLI4M_05535) - 1021632..1022345 (+) 714 WP_048917262.1 YggS family pyridoxal phosphate-dependent enzyme -
  ACLI4M_RS05540 (ACLI4M_05540) - 1022354..1022893 (+) 540 WP_048917250.1 YggT family protein -
  ACLI4M_RS05545 (ACLI4M_05545) - 1022915..1023508 (+) 594 WP_048917251.1 XTP/dITP diphosphatase -
  ACLI4M_RS05550 (ACLI4M_05550) hemW 1023501..1024637 (+) 1137 WP_133622797.1 radical SAM family heme chaperone HemW -
  ACLI4M_RS05555 (ACLI4M_05555) - 1024720..1025433 (-) 714 WP_048917253.1 DUF2884 domain-containing protein -
  ACLI4M_RS05560 (ACLI4M_05560) - 1025548..1025874 (-) 327 WP_048917254.1 YggL family protein -
  ACLI4M_RS05565 (ACLI4M_05565) trmB 1025874..1026593 (-) 720 WP_133622796.1 tRNA (guanosine(46)-N7)-methyltransferase TrmB -

Sequence

Protein


Download         Length: 332 a.a.        Molecular weight: 36343.74 Da        Isoelectric Point: 6.9262

>NTDB_id=1090235 ACLI4M_RS05530 WP_133622798.1 1020618..1021616(-) (pilT) [Erwinia sp. LJJL01]
MDIEEIVALSVKHNAGDLHLCSGHYPFWRCNGRLERIEGEGVMRDEWLESFALRWLSTAQHQELARSGQVDFALTLPDGV
RLRANLFRQRCGLSLALRLIASHCPPLEALHLPAISQTLLQLQEGLVLVVGATGSGKSTTLAALIAAMNQQQRRHILTLE
DPIEFLHSSAQSLIQQREMGQHFTRFGDGLRAALREDPDVILLGELRDAETIQLALTAAETGHLVLATLHTRGAAQAVER
LVDVFPGSEKNLVRSQLAGSLQAVIAQRLVAAQQGRVALFEVLVNSPAVANLIREGKTHQLPALLQTGSQLGMQTFEQSM
AERQRRGLLAAG

Nucleotide


Download         Length: 999 bp        

>NTDB_id=1090235 ACLI4M_RS05530 WP_133622798.1 1020618..1021616(-) (pilT) [Erwinia sp. LJJL01]
ATGGATATTGAAGAAATTGTGGCCCTTAGTGTAAAGCATAATGCAGGCGATCTGCACCTTTGCAGTGGTCATTATCCCTT
CTGGCGCTGTAATGGTCGCCTGGAGCGCATTGAGGGGGAAGGGGTGATGCGCGATGAGTGGCTGGAGAGCTTCGCGCTGC
GCTGGCTGAGCACGGCCCAGCATCAGGAGCTGGCGCGCAGCGGGCAGGTGGATTTTGCCCTGACCCTGCCGGATGGCGTG
CGGCTGCGCGCCAACCTGTTTCGCCAGCGCTGCGGCCTGTCGCTGGCGCTGCGCCTGATTGCCAGCCACTGTCCGCCGCT
GGAAGCGCTGCACCTGCCCGCCATCAGCCAGACGCTGCTACAGCTGCAGGAGGGGCTGGTGCTGGTCGTCGGCGCGACGG
GCAGCGGCAAGTCGACCACGCTGGCGGCGCTGATTGCGGCGATGAATCAGCAGCAGCGGCGCCATATCCTGACGCTGGAG
GACCCGATCGAGTTTCTGCACAGCAGCGCGCAGTCGCTGATCCAGCAGCGTGAAATGGGGCAGCATTTTACCCGCTTTGG
TGATGGGCTGCGCGCGGCGCTGCGTGAGGACCCTGATGTGATCCTGCTGGGCGAGCTGCGCGATGCCGAGACAATCCAGC
TGGCGCTGACCGCCGCGGAGACCGGGCACCTGGTGCTGGCGACGCTGCACACCCGCGGCGCCGCTCAGGCGGTGGAGCGG
CTGGTGGATGTGTTTCCGGGCAGCGAAAAGAATCTGGTGCGCAGCCAGCTGGCAGGCAGTTTACAAGCGGTGATCGCTCA
GCGTTTAGTGGCGGCGCAGCAGGGGCGGGTAGCGCTGTTCGAGGTGTTGGTCAACAGCCCGGCAGTAGCGAACCTGATCC
GCGAAGGAAAAACACACCAGCTGCCCGCGCTGTTGCAGACCGGCAGTCAGTTGGGGATGCAGACCTTCGAGCAGAGCATG
GCGGAGCGGCAGAGAAGGGGGTTATTGGCCGCAGGTTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(81-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Neisseria meningitidis 8013

50

100

0.5
  pilT Neisseria gonorrhoeae MS11

49.699

100

0.497
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

48.795

100

0.488
  pilT Vibrio cholerae strain A1552

48.795

100

0.488
  pilT Pseudomonas stutzeri DSM 10701

45.946

100

0.461
  pilT Acinetobacter baumannii strain A118

45.345

100

0.455
  pilT Acinetobacter baumannii D1279779

45.345

100

0.455
  pilT Acinetobacter baylyi ADP1

45.455

99.398

0.452
  pilT Pseudomonas aeruginosa PAK

44.745

100

0.449
  pilT Acinetobacter nosocomialis M2

45.015

99.699

0.449
  pilT Legionella pneumophila strain ERS1305867

44.411

99.699

0.443
  pilT Legionella pneumophila strain Lp02

44.411

99.699

0.443
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.296

99.699

0.422
  pilU Vibrio cholerae strain A1552

38.889

97.59

0.38
  pilU Pseudomonas stutzeri DSM 10701

38.82

96.988

0.377
  pilU Acinetobacter baylyi ADP1

37.195

98.795

0.367

Multiple sequence alignment