Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   ABWW34_RS00090 Genome accession   NZ_AP031323
Coordinates   17854..18939 (-) Length   361 a.a.
NCBI ID   WP_143584087.1    Uniprot ID   A0AAD1KS38
Organism   Thermus thermophilus strain TF19     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 12854..23939
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABWW34_RS00075 (TthTF19_00120) - 14798..15430 (-) 633 WP_014628814.1 histidine phosphatase family protein -
  ABWW34_RS00080 (TthTF19_00130) purM 15427..16428 (-) 1002 WP_353300382.1 phosphoribosylformylglycinamidine cyclo-ligase -
  ABWW34_RS00085 (TthTF19_00140) gatB 16434..17843 (-) 1410 WP_353300383.1 Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase subunit GatB -
  ABWW34_RS00090 (TthTF19_00150) pilT 17854..18939 (-) 1086 WP_143584087.1 type IV pilus twitching motility protein PilT Machinery gene
  ABWW34_RS00095 (TthTF19_00160) pilF 18952..21618 (-) 2667 WP_353300384.1 type IV pilus assembly ATPase PilB Machinery gene
  ABWW34_RS00100 (TthTF19_00170) - 21615..22100 (-) 486 WP_014628818.1 YqeG family HAD IIIA-type phosphatase -
  ABWW34_RS00105 (TthTF19_00180) pgeF 22135..22863 (-) 729 WP_014628819.1 peptidoglycan editing factor PgeF -
  ABWW34_RS00110 (TthTF19_00190) surE 22860..23615 (-) 756 WP_011173994.1 5'/3'-nucleotidase SurE -
  ABWW34_RS00115 (TthTF19_00200) - 23674..23880 (-) 207 WP_014628821.1 cold-shock protein -

Sequence

Protein


Download         Length: 361 a.a.        Molecular weight: 39934.27 Da        Isoelectric Point: 7.5112

>NTDB_id=108665 ABWW34_RS00090 WP_143584087.1 17854..18939(-) (pilT) [Thermus thermophilus strain TF19]
MAKAPDVVDLLALAVERGASDLVITVGLPPMLKIDGEFHPTEYEPLTPQETRRLMYALMDEKQQRIFEEEKELDFSFSLP
GKGRYRVNVFLQRGSVGGVLRVVPSTVKSFEELGLPKNIAEIAMSPRGLVLVTGPTGSGKSTTLAAMIDYINERKACHIV
TIEDPIEFFHKHKRAIVNQREIGSDTKSFHKALRSVLRQAPDVILVGEMRDYETIAAAITAAETGHLVMGTLHTNSAPET
IDRIVDVFPENQQEQVRVQLSNNLVAVLTQQLLPKAFGGGRVLAYELMIATPAVRALIREGKTHQLRSVIQTGGQYGMIT
MDACLADLYKRKLITYEMGLARAVDPKEFMRLAGAPEGARR

Nucleotide


Download         Length: 1086 bp        

>NTDB_id=108665 ABWW34_RS00090 WP_143584087.1 17854..18939(-) (pilT) [Thermus thermophilus strain TF19]
ATGGCCAAAGCCCCAGACGTGGTGGACCTTTTGGCGCTCGCGGTGGAGCGGGGGGCGAGCGACCTGGTGATCACCGTGGG
CCTGCCCCCCATGCTGAAGATCGACGGGGAGTTCCACCCCACGGAGTACGAGCCCCTCACCCCCCAGGAGACCCGCCGCC
TCATGTACGCCCTCATGGACGAGAAGCAGCAGCGGATCTTTGAGGAGGAGAAGGAGCTGGACTTCTCCTTCAGCCTCCCC
GGGAAGGGGCGGTACCGGGTCAACGTCTTCCTGCAGCGGGGGAGCGTGGGGGGGGTTCTAAGGGTGGTGCCGAGCACCGT
GAAGAGCTTTGAGGAGCTCGGCCTCCCCAAGAACATCGCCGAGATCGCCATGAGCCCAAGGGGCCTCGTCCTGGTCACGG
GGCCCACAGGGTCTGGCAAGAGCACCACCCTGGCCGCCATGATCGACTACATCAACGAGCGCAAGGCCTGCCACATCGTC
ACCATCGAGGACCCCATAGAGTTCTTCCACAAGCACAAGCGGGCCATCGTGAACCAACGGGAGATCGGCTCCGACACCAA
GAGCTTCCACAAGGCGCTCCGCAGCGTCCTCCGCCAGGCCCCGGACGTGATCCTCGTGGGGGAGATGCGGGACTACGAGA
CCATCGCCGCCGCCATCACCGCCGCCGAGACGGGGCACCTGGTCATGGGCACCCTCCACACCAACTCCGCCCCGGAAACC
ATAGACCGCATCGTGGACGTCTTCCCGGAAAACCAGCAGGAACAGGTCCGGGTGCAGCTCTCCAACAACCTGGTGGCCGT
CCTCACGCAACAGCTCCTGCCCAAGGCCTTTGGCGGCGGGCGGGTCCTGGCCTACGAGCTCATGATCGCCACCCCAGCCG
TGAGGGCCCTCATCCGGGAGGGGAAGACCCACCAGTTAAGGAGCGTGATCCAGACGGGCGGCCAGTACGGGATGATCACC
ATGGACGCCTGCCTCGCCGACCTGTACAAGCGCAAGCTCATCACCTACGAGATGGGGCTCGCCAGGGCCGTGGACCCCAA
GGAGTTCATGAGGCTCGCGGGCGCGCCCGAGGGCGCCAGGCGGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(34-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

67.989

97.784

0.665
  pilT Pseudomonas aeruginosa PAK

54.36

95.291

0.518
  pilT Pseudomonas stutzeri DSM 10701

54.07

95.291

0.515
  pilT Acinetobacter baylyi ADP1

54.07

95.291

0.515
  pilT Acinetobacter baumannii strain A118

53.043

95.568

0.507
  pilT Acinetobacter nosocomialis M2

53.043

95.568

0.507
  pilT Acinetobacter baumannii D1279779

53.043

95.568

0.507
  pilT Neisseria meningitidis 8013

52.959

93.629

0.496
  pilT Neisseria gonorrhoeae MS11

52.226

93.352

0.488
  pilT Legionella pneumophila strain ERS1305867

50.872

95.291

0.485
  pilT Legionella pneumophila strain Lp02

50.872

95.291

0.485
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

51.976

91.136

0.474
  pilT Vibrio cholerae strain A1552

51.976

91.136

0.474
  pilU Pseudomonas stutzeri DSM 10701

46.939

95.014

0.446
  pilU Vibrio cholerae strain A1552

44.311

92.521

0.41
  pilU Acinetobacter baylyi ADP1

42.319

95.568

0.404

Multiple sequence alignment