Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   ACLF6M_RS16595 Genome accession   NZ_CP177252
Coordinates   3432965..3434179 (+) Length   404 a.a.
NCBI ID   WP_412027684.1    Uniprot ID   -
Organism   Deinococcus yunweiensis strain KCTC 3955     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3427965..3439179
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACLF6M_RS16575 (ACLF6M_16575) - 3427973..3428758 (-) 786 WP_412027680.1 enoyl-ACP reductase -
  ACLF6M_RS16580 (ACLF6M_16580) pgeF 3428907..3429638 (+) 732 WP_412027681.1 peptidoglycan editing factor PgeF -
  ACLF6M_RS16585 (ACLF6M_16585) - 3429635..3430141 (+) 507 WP_412027682.1 YqeG family HAD IIIA-type phosphatase -
  ACLF6M_RS16590 (ACLF6M_16590) pilB 3430246..3432924 (+) 2679 WP_412027683.1 ATPase, T2SS/T4P/T4SS family Machinery gene
  ACLF6M_RS16595 (ACLF6M_16595) pilT 3432965..3434179 (+) 1215 WP_412027684.1 type IV pilus twitching motility protein PilT Machinery gene
  ACLF6M_RS16600 (ACLF6M_16600) lon 3434288..3436723 (-) 2436 WP_412027685.1 endopeptidase La -
  ACLF6M_RS16605 (ACLF6M_16605) clpX 3436883..3438091 (-) 1209 WP_412027686.1 ATP-dependent Clp protease ATP-binding subunit ClpX -
  ACLF6M_RS16610 (ACLF6M_16610) clpP 3438088..3438699 (-) 612 WP_412027687.1 ATP-dependent Clp protease proteolytic subunit -

Sequence

Protein


Download         Length: 404 a.a.        Molecular weight: 43914.24 Da        Isoelectric Point: 7.5639

>NTDB_id=1086486 ACLF6M_RS16595 WP_412027684.1 3432965..3434179(+) (pilT) [Deinococcus yunweiensis strain KCTC 3955]
MNTSSADITDILRLAAEKGASDVILTVGLPPQFKLHGVYDSQGFGELVGTETRRLMYSMMNEKQQRTFEEKRELDFSFAL
GEKARFRVNAFMQRGNVGGVLRLIPTKIKSAQEMGLPQNVIDIAGAPRGLVLVTGPTGSGKSTTLAAMIDHINTTKKMHI
MTIEDPIEFMHTHKQSIINQREVGSDTMDFNNALRAVLRQAPDVILVGELRDYETIKAAVTAAETGHLVMGTLHTNSAPE
SIDRIVDVFPEEQQEQIRVQLANNLVAVMTQQLLPKLDGGGRILAYELLVANPAVRSLIREGKTYQIVSVMQTGAREGMI
TMDAYLANLYRRRLISFDVGVERAVDPKEFARLANDPTVGSSLPAAGPAMAGGQAGGYGASARPAEATRPSTPTSTTSTS
FGRR

Nucleotide


Download         Length: 1215 bp        

>NTDB_id=1086486 ACLF6M_RS16595 WP_412027684.1 3432965..3434179(+) (pilT) [Deinococcus yunweiensis strain KCTC 3955]
ATGAATACATCCAGCGCCGATATCACCGACATCCTGCGCCTTGCCGCCGAGAAGGGCGCGTCCGATGTGATCCTGACGGT
GGGCCTGCCGCCGCAGTTCAAGTTGCACGGTGTCTACGATTCCCAGGGCTTCGGCGAACTGGTGGGGACTGAGACCCGCC
GCCTGATGTACTCGATGATGAACGAGAAGCAGCAGCGCACCTTCGAGGAGAAGCGCGAGCTGGACTTCTCCTTTGCGCTG
GGTGAGAAGGCCCGCTTCCGTGTGAATGCCTTCATGCAGCGCGGCAACGTGGGCGGCGTCCTGCGTCTGATCCCCACCAA
GATCAAGAGTGCCCAGGAGATGGGCCTGCCTCAGAACGTGATCGACATCGCGGGAGCGCCCCGTGGGCTGGTGCTCGTGA
CTGGCCCCACCGGCTCGGGCAAGTCCACGACGCTGGCAGCCATGATCGATCACATCAACACCACCAAGAAGATGCACATC
ATGACGATCGAGGATCCGATCGAGTTCATGCACACGCACAAGCAGTCGATCATCAACCAGCGCGAGGTCGGTTCCGATAC
CATGGATTTCAACAATGCGCTGCGTGCGGTGCTGCGTCAGGCCCCGGACGTCATTCTGGTGGGTGAGCTCCGCGACTACG
AGACCATCAAGGCTGCCGTGACCGCCGCTGAGACCGGGCACCTGGTCATGGGTACGCTGCATACCAACAGCGCCCCGGAA
TCCATTGACCGCATCGTGGACGTATTCCCGGAAGAGCAGCAGGAGCAGATCCGCGTGCAGCTCGCCAACAATCTGGTGGC
GGTCATGACCCAGCAGCTGCTGCCCAAGCTCGATGGCGGCGGGCGCATCCTGGCCTACGAACTGCTGGTCGCCAACCCCG
CTGTGCGCTCACTGATCCGGGAGGGCAAAACCTACCAGATCGTGTCGGTTATGCAGACCGGCGCCCGCGAGGGCATGATC
ACCATGGATGCCTACCTGGCCAACCTGTACCGGCGCCGCCTGATCTCTTTCGATGTCGGCGTGGAACGCGCCGTCGATCC
CAAGGAGTTCGCCCGCCTGGCAAACGACCCGACGGTGGGCAGCAGTCTCCCCGCCGCTGGCCCCGCCATGGCAGGCGGAC
AGGCCGGCGGATACGGGGCCAGCGCCCGTCCGGCTGAAGCCACCCGTCCGTCGACCCCGACCTCGACCACCTCGACCTCG
TTCGGTCGCCGCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(49-276)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

81.233

92.327

0.75
  pilT Acinetobacter nosocomialis M2

51.744

85.149

0.441
  pilT Acinetobacter baumannii D1279779

51.744

85.149

0.441
  pilT Acinetobacter baumannii strain A118

51.744

85.149

0.441
  pilT Neisseria meningitidis 8013

52.199

84.406

0.441
  pilT Pseudomonas stutzeri DSM 10701

51.453

85.149

0.438
  pilT Pseudomonas aeruginosa PAK

50.872

85.149

0.433
  pilT Acinetobacter baylyi ADP1

50.872

85.149

0.433
  pilT Neisseria gonorrhoeae MS11

51.632

83.416

0.431
  pilT Legionella pneumophila strain ERS1305867

47.965

85.149

0.408
  pilT Legionella pneumophila strain Lp02

47.965

85.149

0.408
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.544

81.436

0.403
  pilT Vibrio cholerae strain A1552

49.544

81.436

0.403
  pilU Acinetobacter baylyi ADP1

43.275

84.653

0.366
  pilU Pseudomonas stutzeri DSM 10701

44.512

81.188

0.361

Multiple sequence alignment