Detailed information NTG1086485

Bioinformatically predicted

Overview

• Name: pilB
• Type: Machinery gene
• Locus tag: ACLF6M_RS16590
• Genome accession: NZ_CP177252
• Coordinates: 3430246..3432924 (+)
• Length: 892 a.a.
• NCBI ID: WP_412027683.1
• Uniprot ID: -
• Organism: Deinococcus yunweiensis strain KCTC 3955
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3425246..3437924

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ACLF6M_RS16560 (ACLF6M_16560)	-	3425407..3426126 (-)	720	WP_412027677.1	IclR family transcriptional regulator	-
ACLF6M_RS16565 (ACLF6M_16565)	-	3426224..3427267 (-)	1044	WP_412027678.1	M42 family metallopeptidase	-
ACLF6M_RS16570 (ACLF6M_16570)	-	3427386..3427943 (-)	558	WP_412027679.1	hypothetical protein	-
ACLF6M_RS16575 (ACLF6M_16575)	-	3427973..3428758 (-)	786	WP_412027680.1	enoyl-ACP reductase	-
ACLF6M_RS16580 (ACLF6M_16580)	pgeF	3428907..3429638 (+)	732	WP_412027681.1	peptidoglycan editing factor PgeF	-
ACLF6M_RS16585 (ACLF6M_16585)	-	3429635..3430141 (+)	507	WP_412027682.1	YqeG family HAD IIIA-type phosphatase	-
ACLF6M_RS16590 (ACLF6M_16590)	pilB	3430246..3432924 (+)	2679	WP_412027683.1	ATPase, T2SS/T4P/T4SS family	Machinery gene
ACLF6M_RS16595 (ACLF6M_16595)	pilT	3432965..3434179 (+)	1215	WP_412027684.1	type IV pilus twitching motility protein PilT	Machinery gene
ACLF6M_RS16600 (ACLF6M_16600)	lon	3434288..3436723 (-)	2436	WP_412027685.1	endopeptidase La	-

Sequence

Protein

• Length: 892 a.a.
• Molecular weight: 97117.62 Da
• Isoelectric Point: 5.2636

>NTDB_id=1086485 ACLF6M_RS16590 WP_412027683.1 3430246..3432924(+) (pilB) [Deinococcus yunweiensis strain KCTC 3955]
MALSIGDRRLGAILLEQGYVNDADLQKALVRHAEVGGRLAEILIGAGMVGEKRIARAIEEALGIPLVNLMVVQPDGQAIM
AVRAQTALSSLAFPFAIEGSTLRVALVDPLSSICIEALEDDSGLMIEPYQALRDEINWAIATHYPELGLTPVAPETVIEG
AGGGKLGQRLISRGLITDAQLQVALDAQQQTGEPLGATLYAQKAISEDQLYEVLADQIGAVYLKNPRDFKPTEEVLGTML
RADALRLSAVPVDESGGSVTVVASDPRKREDIEALIGRRVQFLVAKPRDVESLIEKYYPQRGRLGEQMVQQGTLSRAQLR
EALQVQAREGRVKPLGEVIIELGFAGADEIDTALQKQNSGGGRLEDTLVQSGKLSPEMLARSLAAQLGYEFLDPVQNPPD
PKVALMIPEATARRYAVVPVRLQGESLIVAMKDPRNVFALDDLKLITGREVLPAVMAEKDIIRLIERYFGNKDMADLNQR
LVQESNKRDKDAKRADDVDISAGLDDNAVVRVVDNLIREAALQEASDIHIEPTEHDVRVRYRVDGVLREQNSLPKGAAQS
MLARIKIMGQLDISERRIPQDGRVRFKKGSIDLDLRLSTLPTVYGEKAVMRLLQKASNIPEVEQLGFSEHNYQRYLDVIH
KPNGIFLVTGPTGSGKSFTSFSTLKRIAVPEKNTTTIEDPVEYEIPGIIQSQVNPVAGMTFARALRAFLRQDPDIIFVGE
IRDTETAKIAVEAALTGHLVLATLHTNDAPGAIVRLEEMGVEHFNIGAAVVGVVAQRLVRKVCPDCKAPTNADPDVLRRL
GITERDLRGAQLMRGAGCPRCGGTGYKGRMGIHELMVIDEPLRIAIGSGKNATEINDVAINQCGMKTLRQDGIGKALQGI
TTLEEVLAVTSK

Nucleotide

• Length: 2679 bp

>NTDB_id=1086485 ACLF6M_RS16590 WP_412027683.1 3430246..3432924(+) (pilB) [Deinococcus yunweiensis strain KCTC 3955]
ATGGCTCTCTCAATCGGTGACCGGCGCCTCGGCGCGATCCTGCTCGAACAGGGCTACGTGAACGACGCAGATCTCCAGAA
GGCTCTGGTTCGCCATGCCGAGGTCGGTGGCCGGCTCGCGGAGATCCTGATCGGTGCGGGCATGGTCGGAGAAAAGCGCA
TCGCGCGGGCCATCGAGGAGGCGCTGGGCATTCCGCTGGTGAACCTGATGGTCGTGCAGCCCGACGGGCAGGCGATCATG
GCGGTACGTGCCCAGACGGCCCTGAGCTCACTGGCCTTCCCCTTCGCCATCGAGGGCAGTACGCTGCGGGTCGCGCTGGT
CGATCCGCTGTCGAGCATCTGTATCGAGGCCCTGGAGGACGACAGCGGCCTGATGATCGAGCCGTACCAGGCACTGCGCG
ACGAGATCAACTGGGCGATCGCCACGCACTACCCCGAACTGGGACTCACGCCGGTCGCTCCCGAGACGGTCATTGAGGGT
GCAGGCGGCGGCAAGCTCGGGCAACGTCTGATCTCGCGCGGCCTGATCACTGACGCGCAGCTCCAGGTGGCGCTCGATGC
CCAGCAGCAGACCGGCGAGCCACTGGGAGCGACCCTGTACGCCCAGAAGGCGATCAGCGAGGATCAGCTGTACGAGGTGC
TGGCCGACCAGATTGGCGCGGTGTACCTGAAAAATCCCCGTGACTTCAAGCCCACTGAGGAAGTGCTCGGCACCATGCTG
CGGGCCGATGCCCTGCGGCTCTCGGCGGTGCCGGTCGACGAGAGTGGCGGCAGCGTCACGGTGGTCGCAAGTGATCCCCG
CAAACGCGAGGACATTGAGGCTCTGATCGGCCGCCGCGTGCAGTTCCTGGTGGCCAAACCCAGGGATGTCGAATCGCTGA
TCGAGAAATACTACCCGCAGCGTGGACGGCTGGGCGAGCAGATGGTGCAGCAGGGCACGCTGTCCCGCGCCCAGCTGCGC
GAGGCGCTCCAGGTGCAGGCCCGCGAGGGCCGCGTCAAGCCGCTGGGCGAGGTGATCATCGAGCTGGGCTTCGCCGGCGC
GGACGAGATCGACACGGCCCTCCAGAAGCAGAACTCCGGCGGGGGCCGCCTGGAGGACACGCTGGTGCAGTCCGGCAAGC
TCTCGCCAGAGATGCTGGCCCGCTCGCTGGCCGCTCAGCTCGGCTATGAGTTCCTGGATCCCGTCCAGAACCCGCCGGAT
CCCAAGGTCGCCCTGATGATCCCCGAGGCGACGGCCCGCCGCTACGCGGTGGTGCCGGTGCGGCTCCAGGGCGAGTCCCT
GATCGTTGCCATGAAGGATCCGCGTAATGTCTTCGCGCTGGACGACCTGAAGCTGATCACCGGACGTGAGGTCCTGCCGG
CCGTCATGGCCGAGAAGGACATCATCCGCCTGATCGAGCGCTACTTCGGCAACAAGGACATGGCCGACCTGAACCAGCGC
CTGGTGCAGGAGAGCAACAAGCGCGACAAGGATGCCAAACGTGCCGACGACGTGGACATCTCGGCCGGGCTCGACGACAA
CGCCGTGGTGCGCGTCGTGGATAACCTGATCCGTGAGGCGGCGCTGCAGGAGGCCTCGGACATTCACATCGAGCCGACCG
AACACGATGTCCGGGTGCGCTACCGGGTCGACGGCGTGCTGCGCGAGCAGAACTCGCTGCCCAAGGGGGCTGCGCAGAGC
ATGCTGGCCCGCATCAAGATCATGGGCCAGCTCGACATCAGCGAGCGGCGCATCCCGCAGGACGGCCGCGTGCGCTTCAA
GAAGGGCAGCATCGACCTCGACCTGCGACTGTCCACCCTGCCCACCGTGTACGGCGAGAAGGCCGTCATGCGTCTGTTGC
AGAAGGCGAGCAACATCCCGGAAGTCGAGCAGCTGGGCTTCAGTGAACACAACTACCAGCGCTATCTCGACGTGATCCAC
AAGCCCAACGGCATCTTCCTGGTGACCGGCCCCACGGGTTCGGGCAAGTCCTTCACCAGTTTTTCCACCCTCAAGCGCAT
CGCGGTGCCCGAGAAGAACACGACCACCATCGAAGACCCGGTCGAATACGAGATTCCGGGGATCATCCAGTCGCAGGTGA
ACCCGGTCGCCGGCATGACCTTCGCCCGTGCCCTGCGCGCCTTCCTGCGTCAGGATCCCGACATCATCTTCGTGGGGGAA
ATCCGTGACACCGAGACCGCCAAGATCGCCGTGGAAGCGGCGCTGACCGGTCACCTGGTGCTGGCCACGCTGCACACCAA
CGACGCGCCGGGCGCGATCGTGCGCCTTGAGGAGATGGGCGTCGAGCACTTCAACATCGGTGCGGCCGTGGTGGGTGTGG
TCGCGCAGCGCCTGGTGCGCAAGGTCTGCCCAGACTGCAAGGCCCCCACCAACGCCGACCCCGACGTGCTGCGCCGCCTG
GGGATCACCGAACGTGACCTGCGCGGTGCCCAGCTCATGCGTGGGGCGGGCTGCCCACGCTGCGGCGGCACCGGCTACAA
GGGCCGCATGGGCATCCATGAACTAATGGTCATTGACGAGCCGCTGCGTATCGCTATCGGCTCGGGCAAGAACGCCACCG
AGATCAACGACGTCGCCATCAACCAGTGCGGCATGAAGACCCTGCGCCAGGACGGTATCGGCAAGGCTCTCCAGGGCATC
ACCACGCTGGAAGAAGTGCTCGCCGTCACCAGCAAGTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (516-781)

• Type II secretion system (T2SS), protein E, N-terminal domain (MshEN) (368-472)

• Type II secretion system (T2SS), protein E, N-terminal domain (MshEN) (199-302)

• Type II secretion system (T2SS), protein E, N-terminal domain (MshEN) (43-146)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilB	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	77.578	100	0.776
pilF	Thermus thermophilus HB27	54.637	100	0.548