Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   ACLINX_RS11365 Genome accession   NZ_CP176876
Coordinates   2474474..2475508 (-) Length   344 a.a.
NCBI ID   WP_191953086.1    Uniprot ID   -
Organism   Pseudomonas orientalis strain MPB27     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2469474..2480508
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACLINX_RS11335 (ACLINX_11335) ruvX 2469482..2469919 (+) 438 WP_181282497.1 Holliday junction resolvase RuvX -
  ACLINX_RS11340 (ACLINX_11340) pyrR 2470078..2470584 (+) 507 WP_065934219.1 bifunctional pyr operon transcriptional regulator/uracil phosphoribosyltransferase PyrR -
  ACLINX_RS11345 (ACLINX_11345) - 2470609..2471613 (+) 1005 WP_065885356.1 aspartate carbamoyltransferase catalytic subunit -
  ACLINX_RS11350 (ACLINX_11350) - 2471610..2472881 (+) 1272 WP_411565894.1 dihydroorotase -
  ACLINX_RS11355 (ACLINX_11355) - 2473017..2473445 (-) 429 WP_181282500.1 NINE protein -
  ACLINX_RS11360 (ACLINX_11360) - 2473695..2474333 (+) 639 WP_181282501.1 C40 family peptidase -
  ACLINX_RS11365 (ACLINX_11365) pilT 2474474..2475508 (-) 1035 WP_191953086.1 type IV pilus twitching motility protein PilT Machinery gene
  ACLINX_RS11370 (ACLINX_11370) - 2475566..2476252 (+) 687 WP_191953087.1 YggS family pyridoxal phosphate-dependent enzyme -
  ACLINX_RS11375 (ACLINX_11375) proC 2476286..2477104 (+) 819 WP_191953088.1 pyrroline-5-carboxylate reductase -
  ACLINX_RS11380 (ACLINX_11380) - 2477130..2477717 (+) 588 WP_057723513.1 YggT family protein -
  ACLINX_RS11385 (ACLINX_11385) metX 2477921..2479063 (+) 1143 WP_181282505.1 homoserine O-succinyltransferase MetX -
  ACLINX_RS11390 (ACLINX_11390) metW 2479089..2479709 (+) 621 WP_181282506.1 methionine biosynthesis protein MetW -
  ACLINX_RS11395 (ACLINX_11395) - 2479730..2480164 (+) 435 WP_191956239.1 DUF4426 domain-containing protein -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 37494.26 Da        Isoelectric Point: 6.8393

>NTDB_id=1083496 ACLINX_RS11365 WP_191953086.1 2474474..2475508(-) (pilT) [Pseudomonas orientalis strain MPB27]
MDITELLTASVRRGASDLHLSAGLAPMLRIDGEVWPLDCPVLGGSQVGDLLSPLLNQHQQKDFETSLETDFAFELPGVAR
FRVNVFRQERGVGAVFRTIPSDIQSLENLGLGEVFQRIAQLPRGLVLVTGPTGSGKSTTLAAMIDYLNRHRRQHILTLED
PIEFIHRPKMALINQRQVHRDTHSFSAALRSALREDPDVILVGELRDLETIRLALTAAETGHLVFGTLHTTSAAKTVDRL
VDVFPAGEKAMVRSMLSESLQAVVSQVLVRKIGGGRVAAHEIMLGTPAIRNLIREDKVAQMVSAMQTGGALGMKTLDMSL
KALVNEGVISAEEAREKARVPADI

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=1083496 ACLINX_RS11365 WP_191953086.1 2474474..2475508(-) (pilT) [Pseudomonas orientalis strain MPB27]
ATGGATATCACTGAATTACTCACCGCCAGCGTACGCCGTGGCGCTTCCGACCTGCATCTGTCGGCGGGCCTGGCGCCGAT
GTTGCGCATCGACGGCGAGGTCTGGCCGCTGGATTGCCCGGTGCTGGGCGGGTCGCAGGTAGGGGATTTATTGAGCCCTT
TGCTCAACCAGCACCAACAAAAGGATTTCGAAACATCTCTTGAAACAGACTTTGCCTTCGAACTGCCCGGCGTGGCGCGC
TTTCGCGTAAATGTGTTCCGGCAGGAGCGGGGCGTGGGCGCGGTGTTTCGCACCATCCCCAGCGACATCCAGAGCCTGGA
AAACCTCGGCCTGGGCGAGGTGTTCCAGCGTATCGCGCAGCTGCCGCGCGGCCTGGTGCTGGTGACCGGGCCCACCGGCT
CGGGCAAGTCCACCACCCTGGCTGCGATGATCGATTATCTGAACCGGCATCGACGCCAGCACATCCTCACGCTGGAAGAC
CCCATCGAATTTATCCACAGGCCGAAAATGGCGCTGATCAACCAGCGCCAGGTGCATCGCGATACCCACAGCTTCTCGGC
GGCGCTGCGTTCGGCGTTGCGCGAAGACCCGGATGTGATTCTGGTGGGCGAGCTACGTGACCTGGAAACCATCCGCCTGG
CGCTGACGGCCGCTGAGACCGGGCACCTGGTATTCGGCACTCTGCACACCACCTCGGCGGCAAAGACGGTAGACCGACTG
GTGGACGTGTTCCCGGCCGGGGAAAAGGCCATGGTCCGCTCGATGCTGTCGGAGTCGCTGCAGGCGGTGGTCTCCCAGGT
GCTGGTCAGGAAGATCGGCGGCGGACGGGTCGCGGCTCACGAAATCATGCTGGGCACGCCCGCTATTCGTAACCTGATCC
GCGAAGACAAAGTGGCGCAGATGGTCTCGGCGATGCAGACCGGCGGGGCGTTGGGGATGAAAACGCTGGATATGAGCTTG
AAGGCGCTGGTGAACGAGGGGGTGATCAGCGCAGAGGAGGCGCGGGAGAAGGCCAGGGTGCCGGCAGACATATAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(113-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

73.256

100

0.733
  pilT Pseudomonas stutzeri DSM 10701

70.93

100

0.709
  pilT Acinetobacter nosocomialis M2

70.088

99.128

0.695
  pilT Acinetobacter baumannii D1279779

70.088

99.128

0.695
  pilT Acinetobacter baumannii strain A118

70.088

99.128

0.695
  pilT Acinetobacter baylyi ADP1

69.501

99.128

0.689
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

65.875

97.965

0.645
  pilT Vibrio cholerae strain A1552

65.875

97.965

0.645
  pilT Legionella pneumophila strain Lp02

64.793

98.256

0.637
  pilT Legionella pneumophila strain ERS1305867

64.793

98.256

0.637
  pilT Neisseria meningitidis 8013

62.353

98.837

0.616
  pilT Neisseria gonorrhoeae MS11

62.059

98.837

0.613
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.145

100

0.503
  pilU Vibrio cholerae strain A1552

39.71

100

0.398
  pilU Acinetobacter baylyi ADP1

39.306

100

0.395
  pilU Pseudomonas stutzeri DSM 10701

40

97.384

0.39

Multiple sequence alignment