Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   AACH43_RS17995 Genome accession   NZ_AP029059
Coordinates   3771359..3772462 (+) Length   367 a.a.
NCBI ID   WP_011166152.1    Uniprot ID   Q6MGS0
Organism   Bdellovibrio bacteriovorus strain 109J     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3766359..3777462
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AACH43_RS17975 (Bb109J_c3566) rpmA 3766954..3767214 (-) 261 WP_011166148.1 50S ribosomal protein L27 -
  AACH43_RS17980 (Bb109J_c3567) rplU 3767244..3767807 (-) 564 WP_038450368.1 50S ribosomal protein L21 -
  AACH43_RS17985 (Bb109J_c3568) - 3768015..3769616 (-) 1602 WP_144313886.1 hypothetical protein -
  AACH43_RS17990 (Bb109J_c3569) - 3769694..3771211 (-) 1518 WP_038450370.1 Rne/Rng family ribonuclease -
  AACH43_RS17995 (Bb109J_c3570) pilT 3771359..3772462 (+) 1104 WP_011166152.1 type IV pilus twitching motility protein PilT Machinery gene
  AACH43_RS18000 (Bb109J_c3571) - 3772464..3772841 (-) 378 WP_226987857.1 hypothetical protein -
  AACH43_RS18005 (Bb109J_c3572) deoC 3772901..3773563 (+) 663 WP_038450373.1 deoxyribose-phosphate aldolase -
  AACH43_RS18010 (Bb109J_c3573) - 3773563..3774882 (+) 1320 WP_038450374.1 thymidine phosphorylase -
  AACH43_RS18015 (Bb109J_c3574) - 3774909..3775724 (+) 816 WP_226987858.1 purine-nucleoside phosphorylase -
  AACH43_RS18020 (Bb109J_c3575) - 3775869..3777098 (+) 1230 WP_038450378.1 S8 family peptidase -

Sequence

Protein


Download         Length: 367 a.a.        Molecular weight: 39793.93 Da        Isoelectric Point: 7.9844

>NTDB_id=108336 AACH43_RS17995 WP_011166152.1 3771359..3772462(+) (pilT) [Bdellovibrio bacteriovorus strain 109J]
MATIDELFKLMVEQGASDLHITSGAAPYLRLHGNMVPLNYRELTNQDVQGLLFEILSEKQKKAFVEKWELDFAYTLSGVG
RFRCNIFMQRKGLGAVMRIIPEKIKTAQELGLPPAVMDMIDCDRGLILVTGPTGSGKSTTLAAMIHQINMTREAHIITVE
DPIEFVHPNLKSLVNQREVGSHTKSFANALKAALREDPDILLVGELRDLETISLALTAAETGHIVFGTLHTNSAAKTIDR
IIDVFPAGQQQQIRTMLAESLRGVVAQTLFSRADGQGRVAAYEIMRNTKAISNLVREGKVHQIPSAMQTGATQGMVLFEK
YIEDLVRKGKVSAADAKTFLGQAGGGDTSVQTGMGGAPAGGPRTKVG

Nucleotide


Download         Length: 1104 bp        

>NTDB_id=108336 AACH43_RS17995 WP_011166152.1 3771359..3772462(+) (pilT) [Bdellovibrio bacteriovorus strain 109J]
ATGGCAACAATTGATGAATTGTTCAAACTCATGGTGGAACAAGGTGCCTCCGACTTGCATATCACAAGTGGAGCCGCCCC
TTATTTGCGCCTGCATGGAAACATGGTTCCATTGAACTATCGCGAGCTCACAAATCAGGACGTGCAGGGACTGCTTTTTG
AAATTCTCTCTGAAAAGCAGAAGAAAGCTTTCGTTGAAAAGTGGGAACTGGATTTCGCCTACACTTTGTCAGGTGTCGGA
CGTTTCCGTTGCAATATCTTTATGCAGCGTAAAGGCCTTGGCGCCGTTATGCGTATCATCCCGGAAAAAATCAAAACAGC
ACAGGAACTGGGACTGCCTCCGGCAGTGATGGACATGATCGATTGTGATCGTGGTCTGATTCTGGTTACCGGTCCGACGG
GTTCTGGTAAATCCACAACTCTGGCGGCGATGATTCACCAAATTAACATGACCCGCGAAGCCCATATCATCACGGTGGAA
GACCCGATCGAGTTCGTTCACCCGAACTTGAAGTCTCTGGTCAATCAGCGTGAAGTGGGAAGCCACACCAAAAGTTTTGC
CAACGCCCTGAAAGCGGCCTTGCGTGAAGACCCGGATATTTTGCTGGTGGGTGAGCTTCGTGACTTGGAAACGATTTCTT
TGGCCCTGACTGCCGCAGAAACAGGTCATATCGTTTTCGGAACCCTGCACACCAACAGTGCGGCGAAAACCATCGATCGT
ATCATTGACGTATTCCCTGCCGGTCAACAGCAGCAGATCCGAACGATGCTGGCTGAATCCCTGCGCGGAGTTGTCGCCCA
GACGCTGTTCTCCCGCGCGGACGGACAAGGTCGTGTGGCGGCTTACGAGATCATGAGAAACACCAAAGCCATCTCGAACT
TGGTTCGTGAAGGTAAGGTCCACCAGATCCCATCTGCCATGCAGACCGGTGCCACTCAAGGGATGGTTCTGTTTGAAAAG
TACATTGAAGATCTGGTTCGTAAGGGTAAAGTTTCCGCAGCCGACGCCAAGACCTTCCTGGGTCAGGCCGGTGGTGGTGA
CACCAGCGTGCAGACCGGAATGGGTGGCGCACCTGCTGGCGGTCCGCGCACCAAAGTGGGCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(12-258)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q6MGS0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.22

100

0.515
  pilT Acinetobacter nosocomialis M2

54.925

91.281

0.501
  pilT Acinetobacter baumannii D1279779

54.925

91.281

0.501
  pilT Acinetobacter baumannii strain A118

54.925

91.281

0.501
  pilT Pseudomonas aeruginosa PAK

54.79

91.008

0.499
  pilT Acinetobacter baylyi ADP1

54.491

91.008

0.496
  pilT Vibrio cholerae strain A1552

54.328

91.281

0.496
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

54.328

91.281

0.496
  pilT Pseudomonas stutzeri DSM 10701

54.192

91.008

0.493
  pilT Legionella pneumophila strain ERS1305867

54.407

89.646

0.488
  pilT Legionella pneumophila strain Lp02

54.407

89.646

0.488
  pilT Neisseria gonorrhoeae MS11

50.898

91.008

0.463
  pilT Neisseria meningitidis 8013

50.898

91.008

0.463
  pilU Pseudomonas stutzeri DSM 10701

39.782

100

0.398
  pilU Acinetobacter baylyi ADP1

41.018

91.008

0.373
  pilU Vibrio cholerae strain A1552

41.104

88.828

0.365

Multiple sequence alignment