Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   ACLH0J_RS07450 Genome accession   NZ_CP176771
Coordinates   1614362..1615396 (-) Length   344 a.a.
NCBI ID   WP_411391466.1    Uniprot ID   -
Organism   Pseudomonas sp. MPB23     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1609362..1620396
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACLH0J_RS07420 (ACLH0J_07420) ruvX 1609578..1610015 (+) 438 WP_005792259.1 Holliday junction resolvase RuvX -
  ACLH0J_RS07425 (ACLH0J_07425) pyrR 1610100..1610606 (+) 507 WP_411391461.1 bifunctional pyr operon transcriptional regulator/uracil phosphoribosyltransferase PyrR -
  ACLH0J_RS07430 (ACLH0J_07430) - 1610631..1611635 (+) 1005 WP_411391462.1 aspartate carbamoyltransferase catalytic subunit -
  ACLH0J_RS07435 (ACLH0J_07435) - 1611632..1612903 (+) 1272 WP_411391463.1 dihydroorotase -
  ACLH0J_RS07440 (ACLH0J_07440) - 1612984..1613412 (-) 429 WP_411391464.1 NINE protein -
  ACLH0J_RS07445 (ACLH0J_07445) - 1613663..1614298 (+) 636 WP_411391465.1 C40 family peptidase -
  ACLH0J_RS07450 (ACLH0J_07450) pilT 1614362..1615396 (-) 1035 WP_411391466.1 type IV pilus twitching motility protein PilT Machinery gene
  ACLH0J_RS07455 (ACLH0J_07455) - 1615454..1616140 (+) 687 WP_411391467.1 YggS family pyridoxal phosphate-dependent enzyme -
  ACLH0J_RS07460 (ACLH0J_07460) proC 1616174..1616992 (+) 819 WP_411391468.1 pyrroline-5-carboxylate reductase -
  ACLH0J_RS07465 (ACLH0J_07465) - 1617046..1617633 (+) 588 WP_411391469.1 YggT family protein -
  ACLH0J_RS07470 (ACLH0J_07470) metX 1617753..1618892 (+) 1140 WP_411391470.1 homoserine O-succinyltransferase MetX -
  ACLH0J_RS07475 (ACLH0J_07475) metW 1618900..1619520 (+) 621 WP_321145465.1 methionine biosynthesis protein MetW -
  ACLH0J_RS07480 (ACLH0J_07480) - 1619542..1619976 (+) 435 WP_411391471.1 DUF4426 domain-containing protein -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 37357.12 Da        Isoelectric Point: 6.8386

>NTDB_id=1082857 ACLH0J_RS07450 WP_411391466.1 1614362..1615396(-) (pilT) [Pseudomonas sp. MPB23]
MDITELLMASVRRGASDLHVSAGVAPMLRVDGEVWPLDWPVLSPQQVADLLSPLLNQHQQKDFETSLETDFAFELPGVAR
FRANVFQQDRGMGAVFRTIPGQVQSLESLGLGDVFQRIARLPRGLVLVTGPTGSGKSTTLAAMIDDLNQHRRQHILTLED
PIEFIHTPKTALINQRQVHRDTHSFSTALRSALREDPDVILVGELRDLETIRLALTAAETGHLVLGTLHTTSAAKTVDRL
VDVFPAGEKAMVRTMLSESLQAVVSQVLVKKVGCGRVAAHEIMLGTPAIRNLIREDKVAQIVSAIQTGGALGMKTLDMSL
KALVSAGQISREAAMEKARVPGDI

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=1082857 ACLH0J_RS07450 WP_411391466.1 1614362..1615396(-) (pilT) [Pseudomonas sp. MPB23]
ATGGATATCACTGAATTGCTTATGGCCAGCGTGCGCCGTGGCGCCTCCGACCTGCACGTATCGGCTGGCGTGGCGCCGAT
GTTGCGCGTGGATGGCGAGGTCTGGCCGCTGGATTGGCCGGTGCTTTCACCCCAGCAAGTGGCGGATTTATTAAGCCCTT
TGCTCAACCAGCACCAACAAAAGGATTTCGAAACATCTCTTGAAACGGATTTTGCCTTCGAGCTGCCCGGCGTGGCGCGT
TTTCGGGCGAATGTGTTCCAGCAGGATCGCGGCATGGGGGCGGTGTTCCGCACCATCCCGGGCCAGGTACAGAGCCTGGA
AAGCCTCGGCCTGGGCGACGTGTTCCAGCGTATCGCCCGCTTACCCCGTGGCCTGGTGCTGGTGACCGGGCCGACCGGCT
CCGGCAAGTCCACTACCCTGGCGGCGATGATCGACGACCTCAACCAGCACCGACGCCAGCACATCCTCACGCTGGAAGAC
CCCATCGAATTTATCCACACGCCGAAAACCGCCCTGATCAACCAGCGCCAGGTGCACCGCGACACCCATAGTTTCTCCAC
CGCCCTGCGCTCGGCGCTGCGGGAAGACCCGGATGTGATCCTGGTGGGCGAGTTGCGCGACCTGGAGACCATCCGCCTGG
CGCTGACGGCCGCTGAGACCGGTCACCTGGTATTGGGCACCTTGCACACCACTTCGGCGGCAAAGACCGTAGACCGGCTG
GTAGACGTGTTCCCGGCGGGGGAAAAGGCCATGGTCCGCACGATGCTGTCGGAATCGCTGCAGGCGGTGGTGTCACAGGT
GCTGGTCAAGAAGGTCGGCTGCGGGCGCGTGGCGGCTCACGAAATCATGCTGGGCACGCCGGCCATTCGCAATCTGATCC
GCGAAGACAAGGTGGCGCAGATCGTCTCGGCGATCCAGACCGGTGGTGCGCTGGGGATGAAGACGTTGGATATGAGTTTG
AAGGCGTTGGTCAGCGCGGGGCAGATCAGCCGGGAGGCGGCGATGGAGAAGGCGAGGGTGCCTGGGGATATATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(12-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

71.221

100

0.712
  pilT Pseudomonas stutzeri DSM 10701

70.381

99.128

0.698
  pilT Acinetobacter nosocomialis M2

68.915

99.128

0.683
  pilT Acinetobacter baumannii D1279779

68.622

99.128

0.68
  pilT Acinetobacter baumannii strain A118

68.622

99.128

0.68
  pilT Acinetobacter baylyi ADP1

68.622

99.128

0.68
  pilT Legionella pneumophila strain Lp02

62.722

98.256

0.616
  pilT Legionella pneumophila strain ERS1305867

62.722

98.256

0.616
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

62.908

97.965

0.616
  pilT Vibrio cholerae strain A1552

62.908

97.965

0.616
  pilT Neisseria gonorrhoeae MS11

61.471

98.837

0.608
  pilT Neisseria meningitidis 8013

61.471

98.837

0.608
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.415

99.419

0.491
  pilU Pseudomonas stutzeri DSM 10701

40

100

0.401
  pilU Acinetobacter baylyi ADP1

39.017

100

0.392
  pilU Vibrio cholerae strain A1552

38.841

100

0.39

Multiple sequence alignment