Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   ACAK39_RS17985 Genome accession   NZ_AP029016
Coordinates   3788144..3789124 (-) Length   326 a.a.
NCBI ID   WP_168980337.1    Uniprot ID   -
Organism   Enterobacter asburiae strain CRE21025     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3783144..3794124
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACAK39_RS17955 (EAG21025_35350) - 3784020..3784517 (+) 498 WP_045888327.1 SprT family zinc-dependent metalloprotease -
  ACAK39_RS17960 (EAG21025_35360) endA 3784612..3785319 (+) 708 WP_369604537.1 deoxyribonuclease I -
  ACAK39_RS17965 (EAG21025_35370) rsmE 3785371..3786102 (+) 732 WP_023309126.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  ACAK39_RS17970 (EAG21025_35380) gshB 3786122..3787069 (+) 948 WP_029739222.1 glutathione synthase -
  ACAK39_RS17975 (EAG21025_35390) - 3787157..3787717 (+) 561 WP_008499752.1 YqgE/AlgH family protein -
  ACAK39_RS17980 (EAG21025_35400) ruvX 3787717..3788133 (+) 417 WP_029739223.1 Holliday junction resolvase RuvX -
  ACAK39_RS17985 (EAG21025_35410) pilT 3788144..3789124 (-) 981 WP_168980337.1 type IV pilus twitching motility protein PilT Machinery gene
  ACAK39_RS17990 (EAG21025_35420) - 3789142..3789843 (+) 702 WP_369604538.1 YggS family pyridoxal phosphate-dependent enzyme -
  ACAK39_RS17995 (EAG21025_35430) - 3789865..3790431 (+) 567 WP_010435467.1 YggT family protein -
  ACAK39_RS18000 (EAG21025_35440) yggU 3790428..3790724 (+) 297 WP_023333349.1 DUF167 family protein YggU -
  ACAK39_RS18005 (EAG21025_35450) - 3790728..3791321 (+) 594 WP_369604539.1 XTP/dITP diphosphatase -
  ACAK39_RS18010 (EAG21025_35460) hemW 3791314..3792456 (+) 1143 WP_029739227.1 radical SAM family heme chaperone HemW -
  ACAK39_RS18015 (EAG21025_35470) - 3792520..3792873 (+) 354 WP_369604540.1 endonuclease domain-containing protein -
  ACAK39_RS18020 (EAG21025_35480) - 3792949..3793665 (-) 717 WP_023309135.1 DUF2884 domain-containing protein -
  ACAK39_RS18025 (EAG21025_35490) - 3793722..3794048 (-) 327 WP_023333354.1 YggL family protein -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35593.59 Da        Isoelectric Point: 6.6967

>NTDB_id=108090 ACAK39_RS17985 WP_168980337.1 3788144..3789124(-) (pilT) [Enterobacter asburiae strain CRE21025]
MDVEEIVALSVKHNVSDLHLCSDSPPRWRRLGRLEPAPFPPPDVGALLKAWLNDEQQGTWWAKGQVDFAATVTGDQRLRG
SAFKQMRGVSLTLRLLPRTCPQLSSLGAPRAIPELLSNDAGLILVTGATGSGKSTTLAAMVDFLNHHADGHILTLEDPVE
FIYQSERCLIQQREIGQHSPSFAEALRSALRQDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGASQAIERLVDT
FPAQEKDPVRNQLAGSLRAVLAQRLLPDLQGGRVALYELLVNTAAAANLIREGKTWQLPGIIQTGQQAGMQNFDQSLAER
RAQGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=108090 ACAK39_RS17985 WP_168980337.1 3788144..3789124(-) (pilT) [Enterobacter asburiae strain CRE21025]
ATGGATGTGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAACGTCTCCGATCTACACCTGTGCAGTGATTCACCACCGCG
CTGGCGCAGATTAGGCCGGCTTGAACCCGCTCCCTTTCCGCCTCCCGACGTTGGGGCGTTATTAAAAGCATGGCTTAACG
ATGAGCAGCAGGGAACATGGTGGGCAAAGGGGCAGGTGGATTTTGCCGCGACGGTGACGGGAGACCAGCGGCTGCGCGGC
AGTGCCTTTAAGCAGATGAGAGGTGTCTCTTTGACGCTGCGGCTGTTGCCGCGTACCTGCCCGCAACTCTCTTCGCTGGG
CGCGCCGCGGGCGATCCCGGAACTGTTGTCCAATGACGCCGGGCTGATTCTGGTCACGGGGGCGACCGGCAGCGGCAAAT
CCACTACGCTGGCGGCGATGGTCGATTTTCTCAACCACCATGCTGACGGCCATATCCTCACGCTTGAAGATCCGGTGGAG
TTTATCTACCAGAGCGAACGTTGCCTGATCCAGCAGCGGGAGATAGGCCAGCACAGCCCGTCATTTGCTGAAGCGCTGCG
CAGCGCCTTACGCCAGGATCCGGACGTTATTCTGCTGGGGGAGCTGCGCGACAGCGAAACGATCCGTCTGGCGCTGACGG
CGGCGGAAACCGGACATCTGGTGCTGGCGACGCTGCATACGCGCGGGGCATCGCAGGCGATTGAACGGCTGGTCGATACG
TTCCCGGCGCAGGAGAAAGATCCGGTGCGTAACCAGCTGGCCGGCAGCCTGCGGGCGGTGCTGGCGCAGAGACTGCTTCC
CGATCTACAGGGCGGGCGCGTCGCGTTGTATGAACTGCTGGTGAACACTGCGGCGGCGGCGAATTTGATTCGCGAAGGGA
AAACGTGGCAACTGCCCGGGATCATTCAAACCGGTCAGCAGGCAGGAATGCAGAACTTTGACCAGAGCCTGGCGGAGAGA
CGGGCGCAGGGGCGGCTGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(89-266)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50.459

100

0.506
  pilT Vibrio cholerae strain A1552

50.459

100

0.506
  pilT Pseudomonas stutzeri DSM 10701

48.624

100

0.488
  pilT Legionella pneumophila strain Lp02

49.379

98.773

0.488
  pilT Legionella pneumophila strain ERS1305867

49.379

98.773

0.488
  pilT Acinetobacter baylyi ADP1

48.318

100

0.485
  pilT Acinetobacter baumannii D1279779

48.318

100

0.485
  pilT Acinetobacter baumannii strain A118

48.318

100

0.485
  pilT Neisseria meningitidis 8013

47.866

100

0.482
  pilT Neisseria gonorrhoeae MS11

47.561

100

0.479
  pilT Acinetobacter nosocomialis M2

48.75

98.16

0.479
  pilT Pseudomonas aeruginosa PAK

47.706

100

0.479
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.789

98.773

0.433
  pilU Pseudomonas stutzeri DSM 10701

39.394

100

0.399
  pilU Vibrio cholerae strain A1552

39.441

98.773

0.39
  pilU Acinetobacter baylyi ADP1

36.646

98.773

0.362

Multiple sequence alignment