Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   ACKVEV_RS01275 Genome accession   NZ_CP176417
Coordinates   246074..247126 (+) Length   350 a.a.
NCBI ID   WP_409368579.1    Uniprot ID   -
Organism   Lysinibacillus sp. 38-6     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 241074..252126
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACKVEV_RS01255 - 241723..242292 (-) 570 WP_409368575.1 type II secretion system protein J -
  ACKVEV_RS01260 - 242285..242719 (-) 435 WP_409368576.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  ACKVEV_RS01265 - 243011..244372 (+) 1362 WP_409368577.1 VanW family protein -
  ACKVEV_RS01270 - 244391..246061 (+) 1671 WP_409368578.1 GspE/PulE family protein -
  ACKVEV_RS01275 pilT 246074..247126 (+) 1053 WP_409368579.1 type IV pilus twitching motility protein PilT Machinery gene
  ACKVEV_RS01280 - 247123..248328 (+) 1206 WP_409368580.1 type II secretion system F family protein -
  ACKVEV_RS01285 - 248432..248929 (+) 498 WP_409368581.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  ACKVEV_RS01290 pilM 249034..250008 (+) 975 WP_409368582.1 type IV pilus biogenesis protein PilM -
  ACKVEV_RS01295 - 250005..250601 (+) 597 WP_409368584.1 PilN domain-containing protein -
  ACKVEV_RS01300 - 250598..251299 (+) 702 WP_409368586.1 potassium transporter -
  ACKVEV_RS01305 - 251355..252107 (+) 753 WP_409368588.1 prepilin peptidase -

Sequence

Protein


Download         Length: 350 a.a.        Molecular weight: 39094.86 Da        Isoelectric Point: 6.8734

>NTDB_id=1080004 ACKVEV_RS01275 WP_409368579.1 246074..247126(+) (pilT) [Lysinibacillus sp. 38-6]
MMTTSRTIEELLTRSFEEKASDLHLTMGIPPVYRIHGQLEHYGNKPLTSEELTTMVEAILPDHKRQEFHDKGETDFNYSL
PGKCRFRVNAYHQRNEVTIAARLIEAKIPSIESLNMPQVLYQLAEKPQGLILVTGPTGSGKSTTLAAMIDYINETKSKHI
ITLEDPIEYLHTHKKSVINQREVGVDTQSFANGLRAALRQDPDIILVGEMRDYETISTAITAAETGHLVMATLHTSSAPT
TIDRIIDVFPPHQQGQIRVQLANVLVGIISQRLFIRKDAKGRIAATEILVQAPAIANLIRNEKVHQIPSVMQTSRALGMH
TLESSMQQLISMGKISLEDARPFMNAGDYQ

Nucleotide


Download         Length: 1053 bp        

>NTDB_id=1080004 ACKVEV_RS01275 WP_409368579.1 246074..247126(+) (pilT) [Lysinibacillus sp. 38-6]
ATGATGACAACATCGCGAACTATTGAAGAACTTTTGACACGTTCATTTGAGGAAAAGGCTTCAGACTTACATTTAACAAT
GGGGATTCCACCTGTATACCGCATTCATGGTCAGTTGGAGCATTATGGGAATAAACCACTTACCTCCGAAGAATTAACAA
CAATGGTGGAAGCCATTTTGCCCGATCATAAACGTCAAGAATTTCATGACAAAGGTGAAACGGATTTTAACTATTCATTG
CCCGGGAAATGTCGTTTCCGTGTCAATGCTTACCATCAACGTAATGAGGTGACAATTGCCGCACGTTTAATTGAAGCAAA
GATTCCATCGATTGAATCGTTAAATATGCCACAAGTATTGTATCAGTTAGCTGAAAAACCACAGGGGCTAATTTTAGTAA
CCGGCCCAACCGGTTCAGGGAAATCGACTACACTAGCAGCGATGATTGACTATATTAATGAAACAAAATCTAAGCACATT
ATTACACTTGAAGACCCTATTGAGTATTTACATACCCATAAAAAATCGGTCATCAATCAACGTGAAGTAGGCGTGGATAC
ACAAAGCTTTGCGAATGGTTTACGTGCTGCACTACGACAAGACCCTGACATAATTTTAGTTGGGGAGATGCGTGACTATG
AAACAATTTCAACAGCGATTACAGCTGCAGAGACGGGTCACTTAGTAATGGCGACATTGCATACAAGTAGTGCGCCAACA
ACGATCGATCGAATTATCGACGTCTTCCCACCCCATCAGCAAGGACAAATTCGTGTGCAGTTAGCCAATGTATTGGTAGG
CATAATATCGCAACGATTATTTATTCGAAAAGATGCAAAGGGGCGCATTGCAGCAACGGAAATATTAGTACAGGCACCGG
CAATCGCTAACTTAATTCGAAATGAAAAGGTTCATCAAATTCCAAGTGTCATGCAGACAAGTCGTGCACTCGGTATGCAT
ACATTGGAATCCTCCATGCAGCAGCTAATCTCTATGGGAAAAATTTCTTTAGAGGATGCAAGGCCTTTTATGAATGCGGG
TGACTATCAGTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(118-273)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.976

94

0.489
  pilT Neisseria meningitidis 8013

50.898

95.429

0.486
  pilT Pseudomonas aeruginosa PAK

50.898

95.429

0.486
  pilT Neisseria gonorrhoeae MS11

50.599

95.429

0.483
  pilT Acinetobacter nosocomialis M2

50.299

95.429

0.48
  pilT Acinetobacter baumannii D1279779

50

95.429

0.477
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50

95.429

0.477
  pilT Vibrio cholerae strain A1552

50

95.429

0.477
  pilT Acinetobacter baumannii strain A118

50

95.429

0.477
  pilT Acinetobacter baylyi ADP1

49.701

95.429

0.474
  pilT Pseudomonas stutzeri DSM 10701

49.701

95.429

0.474
  pilT Legionella pneumophila strain ERS1305867

48.328

94

0.454
  pilT Legionella pneumophila strain Lp02

48.328

94

0.454
  pilU Pseudomonas stutzeri DSM 10701

43.243

95.143

0.411
  pilU Vibrio cholerae strain A1552

43.478

92

0.4
  pilU Acinetobacter baylyi ADP1

40.06

94.857

0.38

Multiple sequence alignment