Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   ACKN8R_RS15335 Genome accession   NZ_CP176031
Coordinates   3294461..3295567 (-) Length   368 a.a.
NCBI ID   WP_005461714.1    Uniprot ID   -
Organism   Vibrio parahaemolyticus strain L2171     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3289461..3300567
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACKN8R_RS15305 (ACKN8R_15310) - 3290115..3290612 (+) 498 WP_020835372.1 SprT family zinc-dependent metalloprotease -
  ACKN8R_RS15310 (ACKN8R_15315) endA 3290732..3291427 (+) 696 WP_063517769.1 deoxyribonuclease I -
  ACKN8R_RS15315 (ACKN8R_15320) rsmE 3291552..3292283 (+) 732 WP_005461719.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  ACKN8R_RS15320 (ACKN8R_15325) gshB 3292298..3293248 (+) 951 WP_005425823.1 glutathione synthase -
  ACKN8R_RS15325 (ACKN8R_15330) - 3293362..3293925 (+) 564 WP_005461717.1 YqgE/AlgH family protein -
  ACKN8R_RS15330 (ACKN8R_15335) ruvX 3293974..3294399 (+) 426 WP_005461715.1 Holliday junction resolvase RuvX -
  ACKN8R_RS15335 (ACKN8R_15340) pilU 3294461..3295567 (-) 1107 WP_005461714.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ACKN8R_RS15340 (ACKN8R_15345) pilT 3295598..3296638 (-) 1041 WP_005461712.1 type IV pilus twitching motility protein PilT Machinery gene
  ACKN8R_RS15345 (ACKN8R_15350) - 3296667..3297380 (+) 714 WP_005461710.1 YggS family pyridoxal phosphate-dependent enzyme -
  ACKN8R_RS15350 (ACKN8R_15355) proC 3297500..3298318 (+) 819 WP_005482485.1 pyrroline-5-carboxylate reductase -
  ACKN8R_RS15355 (ACKN8R_15360) - 3298371..3298928 (+) 558 WP_005482440.1 YggT family protein -
  ACKN8R_RS15360 (ACKN8R_15365) yggU 3298928..3299218 (+) 291 WP_005482461.1 DUF167 family protein YggU -
  ACKN8R_RS15365 (ACKN8R_15370) - 3299346..3299777 (+) 432 WP_005461703.1 DUF4426 domain-containing protein -
  ACKN8R_RS15370 (ACKN8R_15375) - 3299895..3300497 (+) 603 WP_015297295.1 XTP/dITP diphosphatase -

Sequence

Protein


Download         Length: 368 a.a.        Molecular weight: 41415.65 Da        Isoelectric Point: 7.0235

>NTDB_id=1079422 ACKN8R_RS15335 WP_005461714.1 3294461..3295567(-) (pilU) [Vibrio parahaemolyticus strain L2171]
MDLNKFLEGMLTLKASDLYITVGAPILFRVDGELRPQGEKLTEHDVAKLLDSAMDPDRRQEFRKSRESNFAIVRDCGRFR
VSAFFQRELPGAVIRRIETIIPTFEQLKLPLVLQDLAIAKRGLVLVVGATGSGKSTTMAAMTGYRNSNKTGHILTVEDPI
EFVHEHKRCIVTQREVGLDTESYEVALKNSLRQAPDMILIGEIRSRETMEYAMTFAETGHLCMATLHANNANQALERILH
LVPKDQKEQFLFDLSMNLKGVIGQQLIRDKNGQGRHGVFEILLNSPRVSDLIRRGDLHELKSTMARSNEFGMLTFDQSLY
KLVMQGKISEEDALHSADSANDLRLMLKTQRGEPFSTGSLANVKIDMD

Nucleotide


Download         Length: 1107 bp        

>NTDB_id=1079422 ACKN8R_RS15335 WP_005461714.1 3294461..3295567(-) (pilU) [Vibrio parahaemolyticus strain L2171]
ATGGATCTGAATAAATTTCTTGAAGGTATGTTGACGCTAAAAGCGTCGGATCTTTACATCACGGTTGGTGCGCCAATTCT
ATTTCGTGTGGATGGCGAATTGCGTCCGCAAGGGGAGAAACTCACCGAACATGATGTTGCAAAGTTGCTTGATAGCGCAA
TGGATCCGGATCGGCGTCAGGAGTTTCGCAAAAGCCGCGAATCAAACTTTGCTATCGTCAGAGATTGTGGTCGCTTCCGT
GTTAGTGCTTTTTTTCAGCGAGAACTGCCGGGTGCAGTTATTCGCCGTATCGAAACCATCATTCCTACTTTTGAGCAGTT
AAAACTGCCTTTAGTGCTGCAAGACTTAGCGATTGCCAAGCGTGGTTTGGTGCTGGTGGTCGGCGCGACTGGCTCGGGTA
AATCGACCACGATGGCGGCGATGACGGGCTATCGAAACAGCAACAAAACCGGTCACATCTTGACGGTCGAAGATCCGATC
GAATTTGTGCATGAGCATAAGCGTTGTATCGTAACTCAGCGTGAGGTGGGTTTGGATACCGAGAGCTATGAAGTCGCATT
GAAAAACTCGCTGCGCCAAGCGCCAGATATGATTTTGATTGGCGAGATCCGTAGCCGAGAAACGATGGAATACGCGATGA
CCTTCGCCGAAACGGGTCACTTGTGTATGGCAACGTTGCACGCCAACAACGCCAACCAAGCGTTAGAGCGTATTCTCCAT
TTGGTGCCTAAAGATCAAAAAGAGCAATTCTTGTTTGATTTGTCGATGAACCTAAAAGGCGTGATTGGTCAGCAGCTGAT
CCGTGATAAGAATGGTCAAGGGCGTCATGGTGTGTTTGAAATCTTGCTAAACAGTCCACGAGTGTCGGATTTGATCCGTC
GTGGTGATTTACACGAATTGAAGTCAACCATGGCACGATCCAATGAGTTTGGCATGCTGACGTTTGACCAATCGCTCTAC
AAGCTGGTGATGCAAGGTAAGATCAGCGAAGAGGATGCATTGCACAGTGCGGATTCAGCCAACGATCTTCGTTTAATGCT
GAAAACACAGCGTGGTGAGCCGTTCTCTACAGGCAGTCTTGCCAACGTCAAAATCGATATGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(116-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

83.152

100

0.832
  pilU Pseudomonas stutzeri DSM 10701

58

95.109

0.552
  pilU Acinetobacter baylyi ADP1

55.271

95.38

0.527
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

45.231

88.315

0.399
  pilT Legionella pneumophila strain ERS1305867

42.773

92.12

0.394
  pilT Legionella pneumophila strain Lp02

42.773

92.12

0.394
  pilT Acinetobacter nosocomialis M2

42.478

92.12

0.391
  pilT Acinetobacter baumannii D1279779

42.478

92.12

0.391
  pilT Acinetobacter baumannii strain A118

42.478

92.12

0.391
  pilT Pseudomonas aeruginosa PAK

42.183

92.12

0.389
  pilT Acinetobacter baylyi ADP1

41.642

92.663

0.386
  pilT Pseudomonas stutzeri DSM 10701

41.003

92.12

0.378
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

41.317

90.761

0.375
  pilT Vibrio cholerae strain A1552

41.317

90.761

0.375
  pilT Neisseria gonorrhoeae MS11

40.361

90.217

0.364
  pilT Neisseria meningitidis 8013

40.361

90.217

0.364

Multiple sequence alignment