Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   ACFLKB_RS15315 Genome accession   NZ_CP175919
Coordinates   3316491..3317543 (-) Length   350 a.a.
NCBI ID   WP_082354854.1    Uniprot ID   A0AAE4JUA1
Organism   Clostridium sp. FAM 1755     
Function   mediate the depolymerization of the type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3311491..3322543
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACFLKB_RS15295 (ACFLKB_15295) ftsZ 3312025..3313134 (-) 1110 WP_163212093.1 cell division protein FtsZ -
  ACFLKB_RS15300 (ACFLKB_15300) ftsA 3313157..3314410 (-) 1254 WP_053467625.1 cell division protein FtsA -
  ACFLKB_RS15305 (ACFLKB_15305) - 3314568..3315842 (-) 1275 WP_053467626.1 glycosyltransferase family 2 protein -
  ACFLKB_RS15310 (ACFLKB_15310) - 3316282..3316416 (+) 135 WP_082354852.1 hypothetical protein -
  ACFLKB_RS15315 (ACFLKB_15315) pilT 3316491..3317543 (-) 1053 WP_082354854.1 type IV pilus twitching motility protein PilT Machinery gene
  ACFLKB_RS15320 (ACFLKB_15320) aroE 3317545..3318321 (-) 777 WP_053467628.1 shikimate dehydrogenase -
  ACFLKB_RS15325 (ACFLKB_15325) sigK 3318449..3319156 (-) 708 WP_409068607.1 RNA polymerase sporulation sigma factor SigK -
  ACFLKB_RS15330 (ACFLKB_15330) - 3319256..3320950 (-) 1695 WP_409068608.1 peptidoglycan D,D-transpeptidase FtsI family protein -
  ACFLKB_RS15335 (ACFLKB_15335) udk 3321035..3321655 (-) 621 WP_053467631.1 uridine kinase -

Sequence

Protein


Download         Length: 350 a.a.        Molecular weight: 39079.16 Da        Isoelectric Point: 8.0876

>NTDB_id=1079160 ACFLKB_RS15315 WP_082354854.1 3316491..3317543(-) (pilT) [Clostridium sp. FAM 1755]
MKQLKELLEKTINFGASDLHLSVGIEPTIRINGQLSKIFKEKLTQEDTNNYAKEILGDEFEDLLKKGEIDTSYSISGLGR
FRVSVFKQRGSIAISLRVVSLKIPNLNELKLPDIVKELTNKKRGLILVTGPTGSGKSTTLAAMINEINNTRSNHIITLED
PIEFLHKHNKCIINQREIGRDTISYKSALKAALREDPDVILIGELRDLEDISIALTAAETGHLVFSTLHTIGGAKTIDRI
VNIFPPHQQEQIRIQVASVLEGIISQQLVHKIDGGRIAALETMVTTNAIRNMIREGKTYQIESSIQTGSKYGMKTMDMSL
AELYKREIISYETALNYSVDKDLITKIINL

Nucleotide


Download         Length: 1053 bp        

>NTDB_id=1079160 ACFLKB_RS15315 WP_082354854.1 3316491..3317543(-) (pilT) [Clostridium sp. FAM 1755]
TTGAAACAATTAAAAGAATTATTAGAAAAGACTATTAACTTTGGTGCTTCAGATTTACATTTGTCTGTAGGAATAGAACC
CACTATAAGAATAAATGGACAATTAAGTAAGATATTTAAAGAAAAGCTTACTCAAGAGGATACTAATAATTATGCTAAAG
AAATATTAGGAGATGAATTTGAAGATTTATTAAAGAAAGGAGAAATAGATACCTCCTACTCTATATCAGGATTAGGTAGA
TTTAGAGTAAGTGTGTTTAAACAAAGAGGAAGTATAGCTATTTCTTTACGGGTTGTAAGTTTAAAGATTCCTAATCTAAA
CGAATTAAAATTGCCTGATATAGTAAAGGAATTAACTAATAAAAAGAGAGGACTTATTTTAGTAACAGGTCCTACAGGGT
CAGGTAAAAGTACTACTTTAGCAGCTATGATTAATGAAATTAATAATACTAGGTCTAATCATATAATAACTTTGGAAGAT
CCAATAGAATTTTTACATAAACATAATAAATGTATAATAAATCAAAGAGAAATAGGTAGAGATACAATTAGTTATAAAAG
TGCTTTAAAAGCAGCCTTAAGGGAAGATCCAGATGTAATATTAATAGGAGAACTTAGAGATTTAGAAGATATTTCTATAG
CTTTAACAGCAGCAGAAACAGGCCATTTGGTATTTTCTACTTTGCATACTATAGGAGGAGCTAAAACTATAGATAGAATT
GTTAATATATTCCCACCACACCAACAAGAACAGATAAGAATACAAGTGGCAAGCGTATTAGAAGGAATAATAAGTCAGCA
ATTAGTACATAAGATTGACGGAGGAAGAATAGCTGCTTTAGAGACTATGGTAACTACAAATGCCATAAGAAATATGATAA
GAGAAGGTAAAACTTATCAAATAGAATCCTCTATACAAACTGGATCTAAATATGGAATGAAAACTATGGATATGTCTCTA
GCAGAATTATATAAAAGAGAAATTATAAGTTATGAAACTGCATTAAATTATTCAGTAGATAAAGATCTAATTACTAAAAT
AATTAATCTATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(92-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter baylyi ADP1

51.051

95.143

0.486
  pilT Legionella pneumophila strain Lp02

48.696

98.571

0.48
  pilT Legionella pneumophila strain ERS1305867

48.696

98.571

0.48
  pilT Pseudomonas aeruginosa PAK

49.702

96

0.477
  pilT Acinetobacter baumannii strain A118

49.55

95.143

0.471
  pilT Acinetobacter baumannii D1279779

49.55

95.143

0.471
  pilT Acinetobacter nosocomialis M2

49.55

95.143

0.471
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.249

95.143

0.469
  pilT Vibrio cholerae strain A1552

49.249

95.143

0.469
  pilT Pseudomonas stutzeri DSM 10701

48.512

96

0.466
  pilT Neisseria meningitidis 8013

48.06

95.714

0.46
  pilT Neisseria gonorrhoeae MS11

48.06

95.714

0.46
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

45.402

99.429

0.451
  pilU Acinetobacter baylyi ADP1

41.399

98

0.406
  pilU Pseudomonas stutzeri DSM 10701

40.597

95.714

0.389
  pilU Vibrio cholerae strain A1552

38.938

96.857

0.377

Multiple sequence alignment