Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   ACFLKC_RS11435 Genome accession   NZ_CP175916
Coordinates   2536591..2537643 (-) Length   350 a.a.
NCBI ID   WP_082354854.1    Uniprot ID   A0AAE4JUA1
Organism   Clostridium sp. FAM 27665     
Function   mediate the depolymerization of the type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2531591..2542643
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACFLKC_RS11415 (ACFLKC_11415) ftsZ 2532125..2533234 (-) 1110 WP_163212093.1 cell division protein FtsZ -
  ACFLKC_RS11420 (ACFLKC_11420) ftsA 2533257..2534510 (-) 1254 WP_053467625.1 cell division protein FtsA -
  ACFLKC_RS11425 (ACFLKC_11425) - 2534668..2535942 (-) 1275 WP_053467626.1 glycosyltransferase family 2 protein -
  ACFLKC_RS11430 (ACFLKC_11430) - 2536382..2536516 (+) 135 WP_082354852.1 hypothetical protein -
  ACFLKC_RS11435 (ACFLKC_11435) pilT 2536591..2537643 (-) 1053 WP_082354854.1 type IV pilus twitching motility protein PilT Machinery gene
  ACFLKC_RS11440 (ACFLKC_11440) aroE 2537645..2538421 (-) 777 WP_053467628.1 shikimate dehydrogenase -
  ACFLKC_RS11445 (ACFLKC_11445) sigK 2538549..2539256 (-) 708 WP_409068607.1 RNA polymerase sporulation sigma factor SigK -
  ACFLKC_RS11450 (ACFLKC_11450) - 2539356..2541050 (-) 1695 WP_409068608.1 peptidoglycan D,D-transpeptidase FtsI family protein -
  ACFLKC_RS11455 (ACFLKC_11455) udk 2541135..2541755 (-) 621 WP_053467631.1 uridine kinase -

Sequence

Protein


Download         Length: 350 a.a.        Molecular weight: 39079.16 Da        Isoelectric Point: 8.0876

>NTDB_id=1079132 ACFLKC_RS11435 WP_082354854.1 2536591..2537643(-) (pilT) [Clostridium sp. FAM 27665]
MKQLKELLEKTINFGASDLHLSVGIEPTIRINGQLSKIFKEKLTQEDTNNYAKEILGDEFEDLLKKGEIDTSYSISGLGR
FRVSVFKQRGSIAISLRVVSLKIPNLNELKLPDIVKELTNKKRGLILVTGPTGSGKSTTLAAMINEINNTRSNHIITLED
PIEFLHKHNKCIINQREIGRDTISYKSALKAALREDPDVILIGELRDLEDISIALTAAETGHLVFSTLHTIGGAKTIDRI
VNIFPPHQQEQIRIQVASVLEGIISQQLVHKIDGGRIAALETMVTTNAIRNMIREGKTYQIESSIQTGSKYGMKTMDMSL
AELYKREIISYETALNYSVDKDLITKIINL

Nucleotide


Download         Length: 1053 bp        

>NTDB_id=1079132 ACFLKC_RS11435 WP_082354854.1 2536591..2537643(-) (pilT) [Clostridium sp. FAM 27665]
TTGAAACAATTAAAAGAATTATTAGAAAAGACTATTAACTTTGGTGCTTCAGATTTACATTTGTCTGTAGGAATAGAACC
CACTATAAGAATAAATGGACAATTAAGTAAGATATTTAAAGAAAAGCTTACTCAAGAGGATACTAATAATTATGCTAAAG
AAATATTAGGAGATGAATTTGAAGATTTATTAAAGAAAGGAGAAATAGATACCTCCTACTCTATATCAGGATTAGGTAGA
TTTAGAGTAAGTGTGTTTAAACAAAGAGGAAGTATAGCTATTTCTTTACGGGTTGTAAGTTTAAAGATTCCTAATCTAAA
CGAATTAAAATTGCCTGATATAGTAAAGGAATTAACTAATAAAAAGAGAGGACTTATTTTAGTAACAGGTCCTACAGGGT
CAGGTAAAAGTACTACTTTAGCAGCTATGATTAATGAAATTAATAATACTAGGTCTAATCATATAATAACTTTGGAAGAT
CCAATAGAATTTTTACATAAACATAATAAATGTATAATAAATCAAAGAGAAATAGGTAGAGATACAATTAGTTATAAAAG
TGCTTTAAAAGCAGCCTTAAGGGAAGATCCAGATGTAATATTAATAGGAGAACTTAGAGATTTAGAAGATATTTCTATAG
CTTTAACAGCAGCAGAAACAGGCCATTTGGTATTTTCTACTTTGCATACTATAGGAGGAGCTAAAACTATAGATAGAATT
GTTAATATATTCCCACCACACCAACAAGAACAGATAAGAATACAAGTGGCAAGCGTATTAGAAGGAATAATAAGTCAGCA
ATTAGTACATAAGATTGACGGAGGAAGAATAGCTGCTTTAGAGACTATGGTAACTACAAATGCCATAAGAAATATGATAA
GAGAAGGTAAAACTTATCAAATAGAATCCTCTATACAAACTGGATCTAAATATGGAATGAAAACTATGGATATGTCTCTA
GCAGAATTATATAAAAGAGAAATTATAAGTTATGAAACTGCATTAAATTATTCAGTAGATAAAGATCTAATTACTAAAAT
AATTAATCTATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(92-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter baylyi ADP1

51.051

95.143

0.486
  pilT Legionella pneumophila strain Lp02

48.696

98.571

0.48
  pilT Legionella pneumophila strain ERS1305867

48.696

98.571

0.48
  pilT Pseudomonas aeruginosa PAK

49.702

96

0.477
  pilT Acinetobacter baumannii strain A118

49.55

95.143

0.471
  pilT Acinetobacter baumannii D1279779

49.55

95.143

0.471
  pilT Acinetobacter nosocomialis M2

49.55

95.143

0.471
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.249

95.143

0.469
  pilT Vibrio cholerae strain A1552

49.249

95.143

0.469
  pilT Pseudomonas stutzeri DSM 10701

48.512

96

0.466
  pilT Neisseria meningitidis 8013

48.06

95.714

0.46
  pilT Neisseria gonorrhoeae MS11

48.06

95.714

0.46
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

45.402

99.429

0.451
  pilU Acinetobacter baylyi ADP1

41.399

98

0.406
  pilU Pseudomonas stutzeri DSM 10701

40.597

95.714

0.389
  pilU Vibrio cholerae strain A1552

38.938

96.857

0.377

Multiple sequence alignment