Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   ACJ2CR_RS37420 Genome accession   NZ_CP175551
Coordinates   9545231..9546343 (+) Length   370 a.a.
NCBI ID   WP_223781741.1    Uniprot ID   -
Organism   Myxococcus fulvus strain KYC1117     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 9540231..9551343
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACJ2CR_RS37400 (ACJ2CR_37400) - 9540510..9541553 (-) 1044 WP_223781740.1 diguanylate cyclase -
  ACJ2CR_RS37405 (ACJ2CR_37405) - 9541664..9542284 (-) 621 WP_223748116.1 tRNA (guanosine(46)-N(7))-methyltransferase TrmB -
  ACJ2CR_RS37410 (ACJ2CR_37410) - 9542351..9543277 (+) 927 WP_223748117.1 bifunctional riboflavin kinase/FAD synthetase -
  ACJ2CR_RS37415 (ACJ2CR_37415) pilB 9543491..9545191 (+) 1701 WP_408889439.1 type IV-A pilus assembly ATPase PilB -
  ACJ2CR_RS37420 (ACJ2CR_37420) pilT 9545231..9546343 (+) 1113 WP_223781741.1 type IV pilus twitching motility protein PilT Machinery gene
  ACJ2CR_RS37425 (ACJ2CR_37425) - 9546402..9547652 (+) 1251 WP_408889440.1 type II secretion system F family protein -
  ACJ2CR_RS37430 (ACJ2CR_37430) - 9547699..9549252 (+) 1554 WP_408889441.1 two-component system sensor histidine kinase NtrB -
  ACJ2CR_RS37435 (ACJ2CR_37435) - 9549258..9550682 (+) 1425 WP_408891819.1 sigma-54-dependent transcriptional regulator -

Sequence

Protein


Download         Length: 370 a.a.        Molecular weight: 40490.76 Da        Isoelectric Point: 8.2875

>NTDB_id=1078105 ACJ2CR_RS37420 WP_223781741.1 9545231..9546343(+) (pilT) [Myxococcus fulvus strain KYC1117]
MANLHQLLKAMVEKGASDLHITTGSPPQLRVDGELVPLKTAPLTPVETKQLCYSILTDAQKHKFEEDNELDLSFGVKGLS
RFRANIFMQRGAVAGAFRTIPFKILTFQELGLPPIVAELVKKPRGLILVTGPTGSGKSTTLASMIDKINTERHEHIMTIE
DPIEYLHPHKNCLVNQREVGADTRNFKTALKYILRQDPDVVLVGELRDLETIEAALTIAETGHICYATLHTNSAVQTINR
VLDVFPPYQQPQVRAQLSFVLEGVMSQALVSKAGAPGRVLALEVMVPNPAIRNLIREDKVHQIYSSMQVGQAKYGMQTFN
QALAALLLRRVISQDEAFGRSSDPEELRNILATGGAVPGAQRPAGGAAGR

Nucleotide


Download         Length: 1113 bp        

>NTDB_id=1078105 ACJ2CR_RS37420 WP_223781741.1 9545231..9546343(+) (pilT) [Myxococcus fulvus strain KYC1117]
GTGGCCAACCTGCACCAGCTCCTCAAGGCGATGGTCGAGAAGGGCGCTTCCGACCTCCACATCACCACCGGCTCCCCGCC
CCAGCTGCGCGTGGACGGCGAGCTCGTCCCCTTGAAGACGGCGCCGCTGACGCCCGTGGAGACCAAGCAGCTCTGCTACT
CCATCCTCACCGACGCCCAGAAGCACAAGTTCGAGGAGGACAACGAGCTGGATCTGTCCTTCGGCGTGAAGGGGCTGTCG
CGCTTTCGCGCCAACATCTTCATGCAGCGTGGCGCGGTGGCCGGCGCGTTCCGCACCATTCCGTTCAAGATTTTGACCTT
CCAGGAGCTGGGCCTGCCGCCCATCGTCGCGGAGCTGGTGAAGAAGCCCCGCGGCCTCATCCTGGTGACGGGCCCCACGG
GCTCGGGCAAGTCCACCACGCTGGCCTCGATGATCGACAAGATCAACACCGAGCGTCATGAGCACATCATGACCATCGAG
GACCCCATCGAGTACCTGCACCCGCACAAGAACTGCCTGGTCAACCAGCGCGAGGTGGGCGCGGACACCCGCAACTTCAA
GACGGCCCTCAAGTACATCCTCCGCCAGGACCCGGACGTGGTGCTGGTCGGTGAGCTCCGCGACCTGGAGACCATCGAGG
CGGCGCTCACCATCGCGGAGACGGGTCACATCTGCTACGCGACGCTGCACACCAACAGCGCGGTGCAGACCATCAACCGC
GTGCTGGACGTGTTCCCGCCGTACCAGCAGCCGCAGGTGCGCGCCCAGCTGTCCTTCGTGCTGGAGGGCGTGATGAGCCA
GGCGCTGGTGTCCAAGGCGGGGGCCCCGGGCCGTGTGCTGGCGCTGGAGGTCATGGTGCCCAACCCCGCCATCCGGAACC
TCATCCGCGAGGACAAGGTCCATCAGATCTACTCGTCCATGCAGGTGGGACAGGCCAAGTACGGCATGCAGACCTTCAAC
CAGGCCCTGGCGGCGCTGCTGCTCCGGCGGGTCATCTCGCAGGACGAGGCCTTCGGTCGCTCCAGCGACCCGGAGGAGCT
GCGCAACATCCTGGCCACGGGCGGGGCGGTGCCGGGTGCCCAGCGGCCGGCCGGCGGAGCGGCGGGTCGTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(8-260)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Legionella pneumophila strain Lp02

53.731

90.541

0.486
  pilT Legionella pneumophila strain ERS1305867

53.731

90.541

0.486
  pilT Acinetobacter nosocomialis M2

51.594

93.243

0.481
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.594

93.243

0.481
  pilT Acinetobacter baumannii D1279779

51.594

93.243

0.481
  pilT Acinetobacter baumannii strain A118

51.594

93.243

0.481
  pilT Acinetobacter baylyi ADP1

51.014

93.243

0.476
  pilT Pseudomonas stutzeri DSM 10701

51.014

93.243

0.476
  pilT Pseudomonas aeruginosa PAK

51.014

93.243

0.476
  pilT Vibrio cholerae strain A1552

49.102

90.27

0.443
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.102

90.27

0.443
  pilT Neisseria meningitidis 8013

44.868

92.162

0.414
  pilT Neisseria gonorrhoeae MS11

44.868

92.162

0.414
  pilU Vibrio cholerae strain A1552

42.857

94.595

0.405
  pilU Pseudomonas stutzeri DSM 10701

40.449

96.216

0.389
  pilU Acinetobacter baylyi ADP1

40.634

93.784

0.381

Multiple sequence alignment