Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   ACJJU9_RS01175 Genome accession   NZ_CP174501
Coordinates   246449..247438 (+) Length   329 a.a.
NCBI ID   WP_407729883.1    Uniprot ID   -
Organism   Pseudomonas helleri strain NF1a     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 241449..252438
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACJJU9_RS01145 (ACJJU9_01145) - 241887..242309 (-) 423 WP_407729881.1 DUF4426 domain-containing protein -
  ACJJU9_RS01150 (ACJJU9_01150) metW 242335..242955 (-) 621 WP_048370967.1 methionine biosynthesis protein MetW -
  ACJJU9_RS01155 (ACJJU9_01155) - 242963..244102 (-) 1140 WP_048385090.1 homoserine O-acetyltransferase -
  ACJJU9_RS01160 (ACJJU9_01160) - 244256..244843 (-) 588 WP_048385088.1 YggT family protein -
  ACJJU9_RS01165 (ACJJU9_01165) proC 244855..245673 (-) 819 WP_048385087.1 pyrroline-5-carboxylate reductase -
  ACJJU9_RS01170 (ACJJU9_01170) - 245705..246391 (-) 687 WP_048385085.1 YggS family pyridoxal phosphate-dependent enzyme -
  ACJJU9_RS01175 (ACJJU9_01175) pilT 246449..247438 (+) 990 WP_407729883.1 type IV pilus twitching motility protein PilT Machinery gene
  ACJJU9_RS01180 (ACJJU9_01180) - 247518..248165 (-) 648 WP_048385081.1 C40 family peptidase -
  ACJJU9_RS01185 (ACJJU9_01185) - 248418..248834 (+) 417 WP_048370954.1 NINE protein -
  ACJJU9_RS01190 (ACJJU9_01190) - 248934..250205 (-) 1272 WP_407729884.1 dihydroorotase -
  ACJJU9_RS01195 (ACJJU9_01195) - 250202..251206 (-) 1005 WP_407729885.1 aspartate carbamoyltransferase catalytic subunit -
  ACJJU9_RS01200 (ACJJU9_01200) pyrR 251219..251737 (-) 519 WP_048385077.1 bifunctional pyr operon transcriptional regulator/uracil phosphoribosyltransferase PyrR -
  ACJJU9_RS01205 (ACJJU9_01205) ruvX 251821..252261 (-) 441 WP_048370946.1 Holliday junction resolvase RuvX -

Sequence

Protein


Download         Length: 329 a.a.        Molecular weight: 35155.52 Da        Isoelectric Point: 6.9345

>NTDB_id=1077361 ACJJU9_RS01175 WP_407729883.1 246449..247438(+) (pilT) [Pseudomonas helleri strain NF1a]
MDIADLLAFSVKHGASDLHLSAGVAPMLRVDGEIQRSDLPMLAPAAARELIHSVMNSAQRSAFESAHEADFSYAVPGLGR
FRVNAFEQQRGAGAVFRSITSQVPSLEGLGLGEVFKGLCGLSQGLVLVTGATGSGKSTTLAAMIDHINATRQQHILTIED
PIEYLHAPRQCLINQREVHRDTHSVAAALRSALREDPDVIMLGELRDLETIRLALTAAETGHLVLATLHTASAAKAVDRI
IDVFAADEKAMVRTMLSESLQGVIAQTLVKRVGGGRVAAHEIMLATPAIRNLIRENKVAQMVSAIQTGAAAGMQTLEMSL
KRLKENGLI

Nucleotide


Download         Length: 990 bp        

>NTDB_id=1077361 ACJJU9_RS01175 WP_407729883.1 246449..247438(+) (pilT) [Pseudomonas helleri strain NF1a]
ATGGATATCGCCGACCTGTTGGCGTTCAGCGTCAAACACGGGGCATCAGACCTGCACCTGTCAGCGGGCGTGGCGCCCAT
GCTGCGGGTGGACGGAGAAATACAGCGTAGCGACCTGCCGATGCTGGCCCCGGCCGCGGCGCGTGAACTTATCCACAGCG
TGATGAATAGCGCACAGCGTTCAGCCTTTGAATCCGCCCACGAAGCTGATTTTTCGTACGCAGTGCCCGGACTGGGACGC
TTTCGGGTCAATGCCTTTGAGCAGCAGCGCGGAGCCGGGGCGGTGTTTCGCAGCATTACGTCGCAAGTCCCCAGCCTGGA
AGGGTTGGGGCTGGGTGAGGTGTTTAAAGGGCTCTGTGGCCTGTCACAGGGGCTGGTGCTGGTCACCGGCGCGACGGGCT
CGGGCAAGAGCACCACCCTGGCCGCAATGATTGATCACATCAACGCAACCCGCCAACAGCACATCCTTACCATCGAAGAC
CCGATTGAGTACCTGCACGCGCCCCGCCAGTGCCTGATCAACCAGCGCGAAGTGCACCGCGACACCCATAGCGTGGCGGC
GGCGCTACGGTCGGCGCTGCGTGAAGACCCGGACGTGATCATGCTCGGAGAACTGCGCGACCTGGAGACCATCCGCCTGG
CGCTGACGGCGGCAGAAACCGGGCACTTGGTGCTGGCGACATTGCACACCGCGTCTGCGGCCAAAGCCGTCGACCGCATC
ATTGACGTGTTCGCCGCCGATGAAAAGGCCATGGTGCGCACGATGTTGTCTGAGTCACTGCAAGGGGTGATTGCGCAAAC
ATTGGTCAAACGGGTGGGGGGAGGCCGTGTCGCGGCGCACGAAATCATGCTGGCCACGCCTGCGATTCGTAACCTGATCC
GGGAAAACAAAGTGGCGCAGATGGTATCGGCGATCCAGACCGGCGCGGCGGCAGGGATGCAGACACTGGAGATGAGCCTG
AAGCGGCTGAAGGAAAACGGCTTGATATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

68.389

100

0.684
  pilT Acinetobacter nosocomialis M2

68.085

100

0.681
  pilT Acinetobacter baumannii D1279779

68.085

100

0.681
  pilT Acinetobacter baumannii strain A118

68.085

100

0.681
  pilT Acinetobacter baylyi ADP1

68.085

100

0.681
  pilT Pseudomonas stutzeri DSM 10701

66.869

100

0.669
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

66.261

100

0.663
  pilT Vibrio cholerae strain A1552

66.261

100

0.663
  pilT Legionella pneumophila strain Lp02

63.222

100

0.632
  pilT Legionella pneumophila strain ERS1305867

63.222

100

0.632
  pilT Neisseria meningitidis 8013

59.697

100

0.599
  pilT Neisseria gonorrhoeae MS11

59.394

100

0.596
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.091

100

0.492
  pilU Pseudomonas stutzeri DSM 10701

43.939

100

0.441
  pilU Vibrio cholerae strain A1552

41.975

98.48

0.413
  pilU Acinetobacter baylyi ADP1

41.304

97.872

0.404

Multiple sequence alignment