Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   ACH36K_RS08300 Genome accession   NZ_CP174370
Coordinates   1782399..1783454 (+) Length   351 a.a.
NCBI ID   WP_411170464.1    Uniprot ID   -
Organism   Clostridium sp. MB05     
Function   mediate the depolymerization of the type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1777399..1788454
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACH36K_RS08280 (ACH36K_08275) - 1777802..1779037 (+) 1236 WP_411170461.1 peptidase U32 family protein -
  ACH36K_RS08285 (ACH36K_08280) udk 1779059..1779676 (+) 618 WP_411170462.1 uridine kinase -
  ACH36K_RS08290 (ACH36K_08285) - 1779784..1781352 (+) 1569 WP_411170673.1 penicillin-binding transpeptidase domain-containing protein -
  ACH36K_RS08295 (ACH36K_08290) sigK 1781587..1782282 (+) 696 WP_411170463.1 RNA polymerase sporulation sigma factor SigK -
  ACH36K_RS08300 (ACH36K_08295) pilT 1782399..1783454 (+) 1056 WP_411170464.1 type IV pilus twitching motility protein PilT Machinery gene
  ACH36K_RS08305 (ACH36K_08300) ftsZ 1783988..1785109 (+) 1122 WP_411170465.1 cell division protein FtsZ -
  ACH36K_RS08310 (ACH36K_08305) spoIIGA 1785334..1786131 (+) 798 WP_411170466.1 sigma-E processing peptidase SpoIIGA -
  ACH36K_RS08315 (ACH36K_08310) sigE 1786140..1786847 (+) 708 WP_411170467.1 RNA polymerase sporulation sigma factor SigE -
  ACH36K_RS08320 (ACH36K_08315) sigG 1786929..1787702 (+) 774 WP_411170468.1 RNA polymerase sporulation sigma factor SigG -
  ACH36K_RS08325 (ACH36K_08320) - 1787770..1788096 (+) 327 WP_411170469.1 YlmC/YmxH family sporulation protein -

Sequence

Protein


Download         Length: 351 a.a.        Molecular weight: 38983.11 Da        Isoelectric Point: 7.2365

>NTDB_id=1076911 ACH36K_RS08300 WP_411170464.1 1782399..1783454(+) (pilT) [Clostridium sp. MB05]
MYVLNDLLNETIEKEASDLHLTVGLPPTIRVNGSLIKLGTEKLSPNALRDFAKEILGDNFELYEKIGEMDTSYSISGTGR
FRVNIFKQRNSDAIAVRVIALKIPTLDELKHPKVIKNIINKQRGLVLVTGPTGSGKSTTLAAMINEINNTREAHIITLED
PIEYLHKHNKCIINQREIGKDSKSYKTALKAILREDPDVILVGEMRDLETISIAITAAETGHLVFSTLHTIGAAKTIDRI
IDVFPPHQQQQIRVQLAAVLQGVISQQLVPTEDNKGRVASLEVMTTTPGIQNLIREGKTHQIESAIQTGGKYGMKTMDMA
LVELYKKGIIDEETALTYSVDRETIKRIMVL

Nucleotide


Download         Length: 1056 bp        

>NTDB_id=1076911 ACH36K_RS08300 WP_411170464.1 1782399..1783454(+) (pilT) [Clostridium sp. MB05]
ATGTATGTGTTAAATGATTTATTAAATGAGACAATAGAAAAAGAAGCGTCGGATTTACACTTAACAGTTGGATTACCCCC
AACAATAAGAGTTAATGGTAGTTTGATTAAATTAGGGACTGAGAAATTAAGTCCTAATGCATTAAGAGATTTTGCTAAGG
AAATACTAGGAGATAATTTTGAATTATATGAAAAGATAGGTGAAATGGATACATCTTACTCTATTTCGGGAACAGGTAGA
TTTAGAGTAAATATATTTAAACAAAGAAATAGTGATGCTATTGCTGTAAGAGTAATTGCATTAAAAATACCGACTTTAGA
TGAGCTAAAACACCCAAAGGTAATAAAGAACATAATAAACAAACAGAGGGGATTAGTATTAGTTACTGGACCAACTGGAA
GTGGTAAAAGTACAACATTAGCAGCTATGATAAATGAAATAAATAATACAAGAGAAGCGCATATTATAACATTAGAAGAT
CCAATAGAATATCTTCATAAGCATAATAAGTGCATAATTAATCAAAGAGAAATAGGAAAAGATAGTAAAAGTTATAAAAC
TGCACTTAAGGCTATTTTAAGAGAGGATCCAGATGTTATACTTGTAGGGGAGATGAGAGACCTCGAAACTATTTCTATTG
CTATAACAGCAGCAGAAACTGGTCATTTAGTATTCTCCACTCTACATACAATAGGAGCTGCTAAAACTATAGATAGAATA
ATAGATGTTTTTCCGCCACACCAACAGCAACAAATAAGAGTTCAACTGGCTGCAGTACTACAGGGAGTTATATCGCAACA
GCTAGTACCAACTGAAGATAATAAGGGGAGAGTTGCATCTTTAGAAGTGATGACTACAACACCAGGAATTCAAAATTTAA
TTAGAGAAGGTAAAACACACCAAATAGAATCAGCTATTCAAACTGGTGGAAAATATGGTATGAAAACAATGGATATGGCA
TTAGTAGAATTATATAAAAAAGGAATTATAGATGAAGAAACTGCACTAACATATTCAGTTGATAGAGAAACTATAAAAAG
AATAATGGTTTTATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(80-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter baylyi ADP1

51.051

94.872

0.484
  pilT Pseudomonas aeruginosa PAK

50.751

94.872

0.481
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50.76

93.732

0.476
  pilT Vibrio cholerae strain A1552

50.76

93.732

0.476
  pilT Acinetobacter nosocomialis M2

48.397

97.721

0.473
  pilT Neisseria meningitidis 8013

48.397

97.721

0.473
  pilT Acinetobacter baumannii D1279779

48.397

97.721

0.473
  pilT Acinetobacter baumannii strain A118

48.397

97.721

0.473
  pilT Neisseria gonorrhoeae MS11

48.256

98.006

0.473
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

47.688

98.575

0.47
  pilT Legionella pneumophila strain Lp02

49.849

94.302

0.47
  pilT Legionella pneumophila strain ERS1305867

49.849

94.302

0.47
  pilT Pseudomonas stutzeri DSM 10701

49.249

94.872

0.467
  pilU Pseudomonas stutzeri DSM 10701

42.609

98.291

0.419
  pilU Vibrio cholerae strain A1552

41.787

98.86

0.413
  pilU Acinetobacter baylyi ADP1

40.708

96.581

0.393

Multiple sequence alignment