Detailed information NTG1076535

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: ACJEH3_RS14000
• Genome accession: NZ_CP174245
• Coordinates: 3093167..3094288 (+)
• Length: 373 a.a.
• NCBI ID: WP_010948029.1
• Uniprot ID: -
• Organism: Legionella pneumophila strain SPF544
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3088167..3099288

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ACJEH3_RS13985 (ACJEH3_13985)	radA	3088673..3090028 (+)	1356	WP_025862411.1	DNA repair protein RadA	-
ACJEH3_RS13990 (ACJEH3_13990)	-	3090025..3090861 (+)	837	WP_010948031.1	phytanoyl-CoA dioxygenase family protein	-
ACJEH3_RS13995 (ACJEH3_13995)	-	3091149..3093077 (+)	1929	WP_010948030.1	FAD-binding oxidoreductase	-
ACJEH3_RS14000 (ACJEH3_14000)	pilU	3093167..3094288 (+)	1122	WP_010948029.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
ACJEH3_RS14005 (ACJEH3_14005)	ankK	3094375..3096300 (+)	1926	WP_010948028.1	Dot/Icm T4SS effector AnkK/LegA5	-
ACJEH3_RS14010 (ACJEH3_14010)	-	3096393..3097688 (-)	1296	WP_014842281.1	serine/threonine transporter	-
ACJEH3_RS14015 (ACJEH3_14015)	-	3097969..3098406 (+)	438	WP_015444141.1	hypothetical protein	-

Sequence

Protein

• Length: 373 a.a.
• Molecular weight: 41657.90 Da
• Isoelectric Point: 5.2999

>NTDB_id=1076535 ACJEH3_RS14000 WP_010948029.1 3093167..3094288(+) (pilU) [Legionella pneumophila strain SPF544]
MDITPFFKLMVDKGASDLFFSVGAPPNIKIEGITSPIGQVPIKSNQMAEIVLSIMNDAQQKEFESKMELNMAFSLAGVGR
FRINLFRQRGEVAMVVRYIKGEIPSIEDLNLPQILKSLVLELRGLVLVVGSTGSGKSTTLAAMINHRNENHRGHILTIED
PIEYIHQHKKSIVDQREIGIDTLSYDNALKNAMREAPDVILIGEIRDLNSMKHAIAYAETGHLCISTLHANNANQALDRV
INFFPDEARQQILLDLSLNLKAIISLRLIPGVDNQRVAAVEILINSPYISDLIEKGKIDDIKEVMARSAEQGMQTFDQAL
FNLYKEGKITKENAIKYADSKNNVGLQIRLGEEKSFNIQDENLQIQPDDIDNV

Nucleotide

• Length: 1122 bp

>NTDB_id=1076535 ACJEH3_RS14000 WP_010948029.1 3093167..3094288(+) (pilU) [Legionella pneumophila strain SPF544]
ATGGATATTACTCCTTTTTTTAAGCTCATGGTCGATAAAGGTGCCTCAGATTTGTTTTTCAGTGTCGGGGCACCACCAAA
TATTAAAATCGAGGGCATCACATCACCTATTGGCCAGGTTCCTATCAAATCGAATCAAATGGCAGAAATTGTTTTATCTA
TTATGAATGACGCACAGCAAAAAGAATTTGAGTCTAAAATGGAACTCAATATGGCTTTTTCATTAGCTGGAGTAGGGCGT
TTCCGAATCAATTTATTCAGGCAACGTGGCGAAGTGGCTATGGTGGTGCGTTACATCAAGGGCGAAATACCTTCTATAGA
AGATTTAAATCTTCCTCAAATTTTAAAGTCTCTGGTCTTGGAGCTGCGTGGTTTAGTATTGGTTGTTGGGTCCACGGGAT
CGGGTAAATCAACGACTCTGGCCGCGATGATCAACCACCGTAATGAAAATCACAGAGGGCATATTTTAACAATAGAGGAT
CCTATTGAATATATACATCAACATAAAAAATCAATAGTTGATCAACGAGAGATAGGTATTGATACTCTAAGCTATGACAA
TGCATTGAAAAACGCGATGAGAGAAGCGCCTGATGTGATATTAATTGGTGAAATTCGTGATCTTAACTCAATGAAACATG
CGATAGCTTATGCTGAAACAGGACATTTGTGCATTTCTACTTTGCATGCCAATAACGCCAATCAGGCTCTGGATAGAGTG
ATTAATTTTTTCCCCGATGAGGCAAGACAACAGATATTATTAGATTTATCTCTTAATTTAAAAGCAATCATTTCATTACG
TTTGATACCAGGAGTTGATAATCAACGTGTCGCAGCGGTGGAGATCTTGATTAACTCTCCTTACATTTCCGATTTGATTG
AAAAAGGGAAGATAGACGATATTAAAGAAGTCATGGCAAGAAGCGCGGAACAAGGCATGCAGACTTTCGATCAGGCTTTA
TTTAATTTATATAAAGAAGGAAAAATTACCAAAGAAAATGCGATTAAGTATGCTGATTCAAAAAACAATGTGGGTTTACA
AATTCGATTGGGCGAAGAAAAAAGCTTTAATATTCAAGATGAGAATTTACAAATACAACCCGATGATATTGATAATGTTT
GA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (43-268)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	55.085	94.906	0.523
pilU	Acinetobacter baylyi ADP1	52.958	95.174	0.504
pilU	Vibrio cholerae strain A1552	48.286	93.834	0.453
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	45.723	90.885	0.416
pilT	Legionella pneumophila strain Lp02	43.275	91.689	0.397
pilT	Legionella pneumophila strain ERS1305867	43.275	91.689	0.397
pilT	Neisseria meningitidis 8013	42.363	93.029	0.394
pilT	Neisseria gonorrhoeae MS11	42.363	93.029	0.394
pilT	Pseudomonas aeruginosa PAK	42.566	91.957	0.391
pilT	Acinetobacter baumannii D1279779	43.413	89.544	0.389
pilT	Acinetobacter nosocomialis M2	43.413	89.544	0.389
pilT	Acinetobacter baumannii strain A118	43.413	89.544	0.389
pilT	Pseudomonas stutzeri DSM 10701	41.983	91.957	0.386
pilT	Acinetobacter baylyi ADP1	41.176	91.153	0.375
pilT	Vibrio cholerae strain A1552	42.249	88.204	0.373
pilT	Vibrio cholerae O1 biovar El Tor strain E7946	42.249	88.204	0.373