Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   AABA48_RS04595 Genome accession   NZ_AP028967
Coordinates   1025541..1026638 (+) Length   365 a.a.
NCBI ID   WP_286349868.1    Uniprot ID   -
Organism   Geobacter sp. 60473     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1020541..1031638
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AABA48_RS04580 (GEO60473_09040) - 1020906..1022777 (+) 1872 WP_338271171.1 DUF2723 domain-containing protein -
  AABA48_RS04585 (GEO60473_09050) aroE 1022780..1023640 (+) 861 WP_286349870.1 shikimate dehydrogenase -
  AABA48_RS04590 (GEO60473_09060) pilB 1023706..1025412 (+) 1707 WP_286349869.1 type IV-A pilus assembly ATPase PilB -
  AABA48_RS04595 (GEO60473_09070) pilT 1025541..1026638 (+) 1098 WP_286349868.1 type IV pilus twitching motility protein PilT Machinery gene
  AABA48_RS04600 (GEO60473_09080) - 1026675..1027892 (+) 1218 WP_286349867.1 type II secretion system F family protein -
  AABA48_RS04605 (GEO60473_09090) - 1027896..1029563 (+) 1668 WP_324310643.1 ATP-binding protein -
  AABA48_RS04610 (GEO60473_09100) - 1029582..1030958 (+) 1377 WP_286349866.1 sigma-54 dependent transcriptional regulator -
  AABA48_RS04615 (GEO60473_09110) - 1031178..1031393 (+) 216 WP_049786599.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -

Sequence

Protein


Download         Length: 365 a.a.        Molecular weight: 40729.12 Da        Isoelectric Point: 7.9038

>NTDB_id=107624 AABA48_RS04595 WP_286349868.1 1025541..1026638(+) (pilT) [Geobacter sp. 60473]
MANMHQLLTELVNRGGSDLHITTNSPPQIRIDGQLIPLEMPPLNAVDTKQLCYSILTEQQKHKFEETNELDLSFGIKGLS
RFRGNVFIQRGAVAGVFRVIPYKILTFEELGLPAVVKELAEKPRGLILVTGPTGSGKSTTLAAIIDKINTERHDHIVTIE
DPIEYLHPHKSCVVNQREVGADTKSFKNALKYILRQDPDVVLVGELRDLETIEAALTLAETGHLCLATLHTNSAVQTINR
IVDVFPPYQQPQVRAQLSFVLEGVMSQTLLPNVSGKGRVLALEVMVPNPAIRNLIREDKIHQIYSQMQVGQEKFGMQTMN
QSLFSLLQKRRISLDVAMARSSDPDELKQMLASAQRPPGQRPQMR

Nucleotide


Download         Length: 1098 bp        

>NTDB_id=107624 AABA48_RS04595 WP_286349868.1 1025541..1026638(+) (pilT) [Geobacter sp. 60473]
ATGGCCAACATGCACCAACTCCTCACGGAACTCGTCAATCGCGGGGGCTCGGACCTTCACATCACCACCAACTCGCCTCC
GCAGATCCGGATCGACGGCCAGTTGATCCCCCTAGAGATGCCGCCGCTCAATGCGGTGGACACCAAGCAGCTCTGCTACA
GCATCCTTACCGAACAGCAGAAGCACAAATTCGAGGAAACCAACGAACTCGACCTTTCTTTCGGCATCAAGGGGCTTTCC
CGTTTCCGGGGGAACGTGTTCATCCAGCGGGGCGCAGTTGCCGGCGTGTTCCGGGTGATTCCCTACAAGATTCTCACTTT
TGAAGAGCTGGGTCTGCCGGCGGTGGTGAAAGAACTGGCGGAGAAACCGCGAGGGCTGATCCTCGTTACCGGTCCCACCG
GCAGCGGCAAGTCGACCACCCTGGCCGCCATCATCGACAAGATCAACACCGAGCGGCACGATCATATCGTAACTATTGAG
GATCCCATCGAGTACCTGCACCCTCACAAAAGTTGCGTGGTGAACCAGCGCGAGGTGGGAGCCGATACGAAAAGCTTCAA
GAACGCCCTGAAATACATCCTGCGTCAGGATCCGGACGTGGTCCTCGTGGGGGAGCTCCGGGATCTGGAGACCATCGAGG
CGGCCCTGACCCTTGCCGAGACCGGTCACCTCTGTCTCGCCACGCTCCACACAAACTCGGCGGTGCAGACCATCAACCGG
ATCGTGGACGTATTCCCCCCCTACCAGCAGCCCCAGGTCCGCGCCCAGCTTTCTTTCGTCCTGGAAGGGGTCATGTCCCA
GACCCTGCTGCCGAACGTGTCGGGCAAGGGGAGGGTGCTGGCCCTGGAGGTGATGGTGCCCAATCCGGCCATCCGTAACC
TGATCCGCGAGGACAAGATCCACCAGATCTATTCCCAGATGCAGGTGGGGCAGGAGAAGTTCGGCATGCAGACCATGAAC
CAGTCGCTGTTCAGTCTGTTGCAGAAGAGACGGATTTCGCTTGATGTGGCCATGGCCCGTTCGTCCGATCCCGACGAACT
CAAGCAGATGCTGGCCAGCGCCCAGCGGCCACCGGGTCAGCGCCCTCAGATGAGGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(13-255)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Legionella pneumophila strain ERS1305867

55.589

90.685

0.504
  pilT Legionella pneumophila strain Lp02

55.589

90.685

0.504
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.705

96.438

0.499
  pilT Acinetobacter baylyi ADP1

50.725

94.521

0.479
  pilT Pseudomonas stutzeri DSM 10701

50.725

94.521

0.479
  pilT Pseudomonas aeruginosa PAK

50.725

94.521

0.479
  pilT Acinetobacter baumannii strain A118

50.145

94.521

0.474
  pilT Acinetobacter nosocomialis M2

50.145

94.521

0.474
  pilT Acinetobacter baumannii D1279779

50.145

94.521

0.474
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.847

89.589

0.447
  pilT Vibrio cholerae strain A1552

49.847

89.589

0.447
  pilT Neisseria meningitidis 8013

48.802

91.507

0.447
  pilT Neisseria gonorrhoeae MS11

47.748

91.233

0.436
  pilU Vibrio cholerae strain A1552

42.571

95.89

0.408
  pilU Pseudomonas stutzeri DSM 10701

41.408

97.26

0.403
  pilU Acinetobacter baylyi ADP1

39.891

100

0.4

Multiple sequence alignment