Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   ACJEH9_RS11370 Genome accession   NZ_CP174222
Coordinates   2564613..2565734 (-) Length   373 a.a.
NCBI ID   WP_010948029.1    Uniprot ID   -
Organism   Legionella pneumophila strain SPF645     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2559613..2570734
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACJEH9_RS11355 (ACJEH9_11355) - 2560495..2560932 (-) 438 WP_015444141.1 hypothetical protein -
  ACJEH9_RS11360 (ACJEH9_11360) - 2561213..2562508 (+) 1296 WP_014842281.1 serine/threonine transporter -
  ACJEH9_RS11365 (ACJEH9_11365) ankK 2562601..2564526 (-) 1926 WP_010948028.1 Dot/Icm T4SS effector AnkK/LegA5 -
  ACJEH9_RS11370 (ACJEH9_11370) pilU 2564613..2565734 (-) 1122 WP_010948029.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ACJEH9_RS11375 (ACJEH9_11375) - 2565824..2567752 (-) 1929 WP_010948030.1 FAD-binding oxidoreductase -
  ACJEH9_RS11380 (ACJEH9_11380) - 2568040..2568876 (-) 837 WP_010948031.1 phytanoyl-CoA dioxygenase family protein -
  ACJEH9_RS11385 (ACJEH9_11385) radA 2568873..2570228 (-) 1356 WP_025862411.1 DNA repair protein RadA -

Sequence

Protein


Download         Length: 373 a.a.        Molecular weight: 41657.90 Da        Isoelectric Point: 5.2999

>NTDB_id=1075559 ACJEH9_RS11370 WP_010948029.1 2564613..2565734(-) (pilU) [Legionella pneumophila strain SPF645]
MDITPFFKLMVDKGASDLFFSVGAPPNIKIEGITSPIGQVPIKSNQMAEIVLSIMNDAQQKEFESKMELNMAFSLAGVGR
FRINLFRQRGEVAMVVRYIKGEIPSIEDLNLPQILKSLVLELRGLVLVVGSTGSGKSTTLAAMINHRNENHRGHILTIED
PIEYIHQHKKSIVDQREIGIDTLSYDNALKNAMREAPDVILIGEIRDLNSMKHAIAYAETGHLCISTLHANNANQALDRV
INFFPDEARQQILLDLSLNLKAIISLRLIPGVDNQRVAAVEILINSPYISDLIEKGKIDDIKEVMARSAEQGMQTFDQAL
FNLYKEGKITKENAIKYADSKNNVGLQIRLGEEKSFNIQDENLQIQPDDIDNV

Nucleotide


Download         Length: 1122 bp        

>NTDB_id=1075559 ACJEH9_RS11370 WP_010948029.1 2564613..2565734(-) (pilU) [Legionella pneumophila strain SPF645]
ATGGATATTACTCCTTTTTTTAAGCTCATGGTCGATAAAGGTGCCTCAGATTTGTTTTTCAGTGTCGGGGCACCACCAAA
TATTAAAATCGAGGGCATCACATCACCTATTGGCCAGGTTCCTATCAAATCGAATCAAATGGCAGAAATTGTTTTATCTA
TTATGAATGACGCACAGCAAAAAGAATTTGAGTCTAAAATGGAACTCAATATGGCTTTTTCATTAGCTGGAGTAGGGCGT
TTCCGAATCAATTTATTCAGGCAACGTGGCGAAGTGGCTATGGTGGTGCGTTACATCAAGGGCGAAATACCTTCTATAGA
AGATTTAAATCTTCCTCAAATTTTAAAGTCTCTGGTCTTGGAGCTGCGTGGTTTAGTATTGGTTGTTGGGTCCACGGGAT
CGGGTAAATCAACGACTCTGGCCGCGATGATCAACCACCGTAATGAAAATCACAGAGGGCATATTTTAACAATAGAGGAT
CCTATTGAATATATACATCAACATAAAAAATCAATAGTTGATCAACGAGAGATAGGTATTGATACTCTAAGCTATGACAA
TGCATTGAAAAACGCGATGAGAGAAGCGCCTGATGTGATATTAATTGGTGAAATTCGTGATCTTAACTCAATGAAACATG
CGATAGCTTATGCTGAAACAGGACATTTGTGCATTTCTACTTTGCATGCCAATAACGCCAATCAGGCTCTGGATAGAGTG
ATTAATTTTTTCCCCGATGAGGCAAGACAACAGATATTATTAGATTTATCTCTTAATTTAAAAGCAATCATTTCATTACG
TTTGATACCAGGAGTTGATAATCAACGTGTCGCAGCGGTGGAGATCTTGATTAACTCTCCTTACATTTCCGATTTGATTG
AAAAAGGGAAGATAGACGATATTAAAGAAGTCATGGCAAGAAGCGCGGAACAAGGCATGCAGACTTTCGATCAGGCTTTA
TTTAATTTATATAAAGAAGGAAAAATTACCAAAGAAAATGCGATTAAGTATGCTGATTCAAAAAACAATGTGGGTTTACA
AATTCGATTGGGCGAAGAAAAAAGCTTTAATATTCAAGATGAGAATTTACAAATACAACCCGATGATATTGATAATGTTT
GA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(43-268)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

55.085

94.906

0.523
  pilU Acinetobacter baylyi ADP1

52.958

95.174

0.504
  pilU Vibrio cholerae strain A1552

48.286

93.834

0.453
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

45.723

90.885

0.416
  pilT Legionella pneumophila strain Lp02

43.275

91.689

0.397
  pilT Legionella pneumophila strain ERS1305867

43.275

91.689

0.397
  pilT Neisseria meningitidis 8013

42.363

93.029

0.394
  pilT Neisseria gonorrhoeae MS11

42.363

93.029

0.394
  pilT Pseudomonas aeruginosa PAK

42.566

91.957

0.391
  pilT Acinetobacter baumannii D1279779

43.413

89.544

0.389
  pilT Acinetobacter nosocomialis M2

43.413

89.544

0.389
  pilT Acinetobacter baumannii strain A118

43.413

89.544

0.389
  pilT Pseudomonas stutzeri DSM 10701

41.983

91.957

0.386
  pilT Acinetobacter baylyi ADP1

41.176

91.153

0.375
  pilT Vibrio cholerae strain A1552

42.249

88.204

0.373
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

42.249

88.204

0.373

Multiple sequence alignment