Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   ACI65L_RS21205 Genome accession   NZ_CP174195
Coordinates   4716395..4717411 (-) Length   338 a.a.
NCBI ID   WP_226294474.1    Uniprot ID   -
Organism   Pectobacterium carotovorum strain PCC27     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4711395..4722411
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACI65L_RS21190 (ACI65L_004225) nsrR 4711769..4712194 (-) 426 WP_010279087.1 nitric oxide-sensing transcriptional repressor NsrR -
  ACI65L_RS21195 (ACI65L_004226) - 4712489..4714060 (+) 1572 WP_010294989.1 methyl-accepting chemotaxis protein -
  ACI65L_RS21200 (ACI65L_004227) - 4714594..4716426 (+) 1833 WP_010294991.1 diguanylate cyclase -
  ACI65L_RS21205 (ACI65L_004228) pilT 4716395..4717411 (-) 1017 WP_226294474.1 type IV pilus twitching motility protein PilT Machinery gene
  ACI65L_RS21210 (ACI65L_004229) - 4717433..4718146 (+) 714 WP_201878023.1 YggS family pyridoxal phosphate-dependent enzyme -
  ACI65L_RS21215 (ACI65L_004230) proC 4718244..4719065 (+) 822 WP_161510110.1 pyrroline-5-carboxylate reductase -
  ACI65L_RS21220 (ACI65L_004231) - 4719115..4719669 (+) 555 WP_010295003.1 YggT family protein -
  ACI65L_RS21225 (ACI65L_004232) yggU 4719666..4719956 (+) 291 WP_010295005.1 DUF167 family protein YggU -
  ACI65L_RS21230 (ACI65L_004233) - 4719988..4720581 (+) 594 WP_039507057.1 XTP/dITP diphosphatase -
  ACI65L_RS21235 (ACI65L_004234) hemW 4720574..4721713 (+) 1140 WP_201878021.1 radical SAM family heme chaperone HemW -
  ACI65L_RS21240 (ACI65L_004235) - 4721948..4722208 (+) 261 WP_072040255.1 type II toxin-antitoxin system ParD family antitoxin -

Sequence

Protein


Download         Length: 338 a.a.        Molecular weight: 36872.16 Da        Isoelectric Point: 6.4929

>NTDB_id=1074832 ACI65L_RS21205 WP_226294474.1 4716395..4717411(-) (pilT) [Pectobacterium carotovorum strain PCC27]
MELDEWMARSVKHNASDLHLCSGHPPVLRVDGRLQPENTLPRLTPDQVAQWCDAWLEPAQCEQLRQAGQVDGALMLPDGQ
RLRVNVFRQREGLSAALRVIPSVQPSLDALHAPPVFSTLLEKPNGLILITGATGSGKSTTLAAMLGALNDRCDRHVITLE
DPIEFIHTSRRCLIQQREIGAHSASFAQALRAALREDPDVILLGELRDTETIRLALTAAETGHLVLSTLHTRSASQAVDR
LIDVFPGEEKAYVRSQLATCLQAVVTQKLLPAEQGGRIALYEVLTATAAVSNLIREGKTHQLPGLIQTGAAAGMQTFEQS
YQQRCRDGLISRSCDLAV

Nucleotide


Download         Length: 1017 bp        

>NTDB_id=1074832 ACI65L_RS21205 WP_226294474.1 4716395..4717411(-) (pilT) [Pectobacterium carotovorum strain PCC27]
ATGGAATTGGATGAGTGGATGGCGCGTAGTGTAAAACATAATGCCTCGGATCTGCACCTTTGTAGCGGTCATCCGCCGGT
GTTACGTGTGGACGGGCGGCTACAGCCTGAAAATACCTTACCACGTTTAACACCGGATCAGGTGGCACAATGGTGCGATG
CCTGGCTTGAACCGGCTCAGTGCGAGCAACTGCGGCAGGCTGGTCAGGTGGACGGGGCATTAATGCTGCCCGATGGACAA
CGCCTGCGGGTGAATGTGTTTCGCCAGCGGGAAGGGCTGTCTGCGGCGTTGCGTGTTATCCCGTCCGTTCAACCTTCATT
GGATGCGCTACATGCGCCACCCGTTTTCTCGACGCTGCTGGAGAAACCGAACGGACTGATCCTGATTACCGGGGCGACGG
GGAGCGGCAAATCCACGACGCTGGCGGCCATGCTTGGGGCGTTAAACGATCGCTGCGATCGCCATGTGATTACGCTGGAG
GACCCGATCGAGTTTATCCATACCAGCCGACGCTGCCTGATTCAACAGCGGGAGATCGGTGCGCACAGCGCGTCGTTTGC
ACAGGCGCTACGGGCTGCATTGCGGGAGGATCCCGATGTCATTCTGCTGGGAGAACTGCGCGATACGGAGACGATTCGGC
TGGCGCTGACGGCGGCGGAAACCGGCCATCTGGTGTTGTCGACGCTGCATACCCGTAGCGCATCGCAAGCGGTTGATCGT
CTGATTGACGTCTTTCCCGGTGAAGAGAAAGCCTATGTTCGCAGCCAGTTAGCCACCTGTTTGCAGGCAGTGGTGACGCA
AAAACTCTTGCCTGCGGAGCAAGGGGGGCGAATCGCGCTTTATGAAGTGCTGACCGCCACCGCAGCGGTCAGTAACCTGA
TTCGGGAAGGGAAAACGCACCAGCTTCCCGGCCTGATTCAGACGGGAGCCGCTGCGGGAATGCAAACGTTTGAGCAAAGC
TACCAGCAGCGCTGTCGGGATGGGCTTATTTCACGCAGTTGCGACCTTGCCGTTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

51.818

97.633

0.506
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

51.818

97.633

0.506
  pilT Neisseria meningitidis 8013

50.301

98.225

0.494
  pilT Neisseria gonorrhoeae MS11

50

98.225

0.491
  pilT Pseudomonas stutzeri DSM 10701

49.102

98.817

0.485
  pilT Acinetobacter baylyi ADP1

49.245

97.929

0.482
  pilT Pseudomonas aeruginosa PAK

49.096

98.225

0.482
  pilT Legionella pneumophila strain ERS1305867

48.494

98.225

0.476
  pilT Legionella pneumophila strain Lp02

48.494

98.225

0.476
  pilT Acinetobacter nosocomialis M2

48.64

97.929

0.476
  pilT Acinetobacter baumannii D1279779

48.64

97.929

0.476
  pilT Acinetobacter baumannii strain A118

48.64

97.929

0.476
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.169

98.225

0.414
  pilU Pseudomonas stutzeri DSM 10701

39.157

98.225

0.385
  pilU Vibrio cholerae strain A1552

38.855

98.225

0.382
  pilU Acinetobacter baylyi ADP1

38.199

95.266

0.364

Multiple sequence alignment