Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   RGQ30_RS04340 Genome accession   NZ_AP028947
Coordinates   955049..956083 (+) Length   344 a.a.
NCBI ID   WP_130558157.1    Uniprot ID   -
Organism   Limnobacter thiooxidans strain CS-K2     
Function   mediate the depolymerization of the type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 950049..961083
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RGQ30_RS04320 (RGQ30_08520) - 951570..952514 (+) 945 WP_130558161.1 LysR substrate-binding domain-containing protein -
  RGQ30_RS04325 (RGQ30_08530) ubiA 952517..953380 (+) 864 WP_130558160.1 4-hydroxybenzoate octaprenyltransferase -
  RGQ30_RS04330 (RGQ30_08540) proC 953461..954282 (-) 822 WP_338284791.1 pyrroline-5-carboxylate reductase -
  RGQ30_RS04335 (RGQ30_08550) - 954317..955009 (-) 693 WP_130558158.1 YggS family pyridoxal phosphate-dependent enzyme -
  RGQ30_RS04340 (RGQ30_08560) pilT 955049..956083 (+) 1035 WP_130558157.1 type IV pilus twitching motility protein PilT Machinery gene
  RGQ30_RS04345 (RGQ30_08570) - 956142..956630 (+) 489 WP_130558714.1 glutathione peroxidase -
  RGQ30_RS04350 (RGQ30_08580) - 956631..957992 (-) 1362 WP_130558156.1 THUMP domain-containing class I SAM-dependent RNA methyltransferase -
  RGQ30_RS04355 (RGQ30_08590) - 958013..958444 (-) 432 WP_130558155.1 CopD family protein -
  RGQ30_RS04360 (RGQ30_08600) - 958471..959727 (-) 1257 WP_130558154.1 glutamate-5-semialdehyde dehydrogenase -
  RGQ30_RS04365 (RGQ30_08610) holA 959766..960806 (-) 1041 WP_130558153.1 DNA polymerase III subunit delta -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 37787.28 Da        Isoelectric Point: 6.5640

>NTDB_id=107481 RGQ30_RS04340 WP_130558157.1 955049..956083(+) (pilT) [Limnobacter thiooxidans strain CS-K2]
MTLTELLTWAVEEHASDLHLSADMPPMIRVDGEVRRLSDAVLGHREIYRLIQSSMSDVQWQAWEQSHECDYSFAVEGLSR
FRVNAFMQSRGASAAFRVVPSRVKTLEELEAPSVFRTIAREPNGLVLVTGPTGSGKSTTLAAMVDDVNRHQKAHILTIED
PIEFLHTPVNCVVNQRELGEHTHSFGAALRSALREDPDVILVGEMRDLETIQLALTAAETGHLVFATLHTSSAPKTIDRI
IDAFPSADKSMVRAMLSESLRAVICQTLLKKPGGGRLAAHEIMLATPPIRNLIREGKIAQMMSSIQTGSQHGMQTLEQSV
QRLLMTGKITRDVAASVLSGSNQP

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=107481 RGQ30_RS04340 WP_130558157.1 955049..956083(+) (pilT) [Limnobacter thiooxidans strain CS-K2]
ATGACACTGACCGAGTTGCTTACCTGGGCTGTGGAGGAGCATGCCTCTGATCTTCATTTGTCGGCTGACATGCCACCCAT
GATCCGCGTGGACGGCGAAGTGCGTCGGCTAAGCGATGCAGTATTGGGTCACCGGGAAATTTACAGACTGATTCAAAGCT
CGATGAGCGATGTGCAATGGCAGGCGTGGGAACAATCGCACGAATGTGATTACAGCTTCGCAGTAGAGGGTTTGTCGCGT
TTCCGGGTGAATGCATTCATGCAAAGCCGGGGGGCATCTGCTGCGTTTCGTGTGGTGCCCAGCCGGGTCAAAACACTGGA
AGAGCTTGAAGCGCCTTCGGTATTCAGAACCATTGCGCGGGAGCCCAATGGCCTGGTGCTGGTGACAGGGCCCACGGGTT
CTGGTAAAAGCACCACGCTGGCTGCAATGGTAGACGATGTGAACCGGCACCAGAAGGCGCACATTCTGACCATTGAAGAT
CCGATCGAGTTTTTGCACACCCCCGTGAATTGCGTGGTGAATCAGCGCGAGCTGGGTGAGCACACCCATTCTTTTGGTGC
AGCTTTGCGGTCTGCACTGCGGGAAGATCCGGATGTGATTCTGGTCGGTGAAATGCGCGACCTTGAAACCATTCAACTGG
CCCTGACTGCCGCTGAAACCGGGCACCTGGTGTTTGCAACCCTGCACACGTCGTCTGCCCCCAAAACCATTGACCGGATC
ATTGATGCATTTCCATCGGCCGACAAATCCATGGTGCGGGCCATGTTGTCAGAATCTCTGCGCGCTGTGATTTGCCAGAC
CCTTTTAAAGAAGCCGGGTGGTGGACGACTGGCTGCCCATGAAATCATGCTGGCCACACCGCCTATTCGAAACCTGATCC
GCGAGGGCAAGATCGCCCAGATGATGTCCTCGATCCAGACCGGTTCGCAGCATGGCATGCAGACGCTGGAGCAGTCGGTT
CAGCGCTTGTTGATGACGGGAAAAATCACGCGGGATGTGGCAGCCTCGGTGTTGTCGGGCTCCAACCAACCCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(7-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter baylyi ADP1

65.282

97.965

0.64
  pilT Pseudomonas stutzeri DSM 10701

64.072

97.093

0.622
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

64.742

95.64

0.619
  pilT Vibrio cholerae strain A1552

64.742

95.64

0.619
  pilT Acinetobacter baumannii D1279779

63.939

95.93

0.613
  pilT Acinetobacter nosocomialis M2

63.939

95.93

0.613
  pilT Acinetobacter baumannii strain A118

63.939

95.93

0.613
  pilT Pseudomonas aeruginosa PAK

62.575

97.093

0.608
  pilT Legionella pneumophila strain Lp02

59.88

97.093

0.581
  pilT Legionella pneumophila strain ERS1305867

59.88

97.093

0.581
  pilT Neisseria meningitidis 8013

59.104

97.384

0.576
  pilT Neisseria gonorrhoeae MS11

58.507

97.384

0.57
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

48.943

96.221

0.471
  pilU Vibrio cholerae strain A1552

40.483

96.221

0.39
  pilU Pseudomonas stutzeri DSM 10701

39.104

97.384

0.381
  pilU Acinetobacter baylyi ADP1

38.509

93.605

0.36

Multiple sequence alignment