Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   ACIUZZ_RS00985 Genome accession   NZ_CP174122
Coordinates   211982..213037 (+) Length   351 a.a.
NCBI ID   WP_026393398.1    Uniprot ID   -
Organism   Acetobacterium dehalogenans DSM 11527     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 206982..218037
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACIUZZ_RS00975 (ACIUZZ_00975) - 208035..210266 (+) 2232 WP_026393396.1 EAL domain-containing protein -
  ACIUZZ_RS00980 (ACIUZZ_00980) - 210288..211970 (+) 1683 WP_026393397.1 GspE/PulE family protein -
  ACIUZZ_RS00985 (ACIUZZ_00985) pilT 211982..213037 (+) 1056 WP_026393398.1 type IV pilus twitching motility protein PilT Machinery gene
  ACIUZZ_RS00990 (ACIUZZ_00990) - 213471..214037 (+) 567 WP_051355586.1 type II secretion system protein -
  ACIUZZ_RS00995 (ACIUZZ_00995) - 214355..214753 (+) 399 WP_026393399.1 hypothetical protein -
  ACIUZZ_RS01000 (ACIUZZ_01000) - 214812..215576 (+) 765 WP_035354893.1 prepilin peptidase -
  ACIUZZ_RS01005 (ACIUZZ_01005) - 215610..216794 (+) 1185 WP_026393401.1 type II secretion system F family protein -
  ACIUZZ_RS01010 (ACIUZZ_01010) pilM 216806..218014 (+) 1209 WP_414149986.1 type IV pilus biogenesis protein PilM -

Sequence

Protein


Download         Length: 351 a.a.        Molecular weight: 38949.35 Da        Isoelectric Point: 6.3683

>NTDB_id=1074419 ACIUZZ_RS00985 WP_026393398.1 211982..213037(+) (pilT) [Acetobacterium dehalogenans DSM 11527]
MTSFLDLVNYGRRNDCSDIHLSRELPPVFRKNGKLYQSDFEHNPQIIENLIFSLLTPDEQKLLVSGTDLDFCHVTPEGLR
QRVNVYKQQGSYAAAIRLLNDTIPSFEDLKLPPIIRKLASEPRGLILITGPTGSGKSTTLASMIDYINTNRAVHILTIED
PVEYKHEHKHSIVHQREIGVDASSFASALRSSLREDPDVILVGEMRDYETVSAAVTAAETGHLVLSTLHTIGAANTIDRI
IDVFPPHSQQQIRTQLSSILKGVVTQQLVPLASGGGRMAALEVLIGTDAVLNLIRENKTHQLASVMQTGGRDGMKTLNAD
LAMLVRDQKITYEVGLEWASDKQEFNQYFGR

Nucleotide


Download         Length: 1056 bp        

>NTDB_id=1074419 ACIUZZ_RS00985 WP_026393398.1 211982..213037(+) (pilT) [Acetobacterium dehalogenans DSM 11527]
ATGACAAGTTTTTTAGATCTGGTCAACTACGGCCGCCGCAACGATTGTTCGGATATTCATTTATCCCGGGAGCTGCCGCC
GGTTTTTCGCAAAAACGGCAAGCTTTATCAAAGTGATTTTGAACACAATCCGCAGATTATTGAAAACCTGATTTTCAGCT
TGCTGACCCCCGATGAACAGAAATTGCTGGTGTCCGGCACCGATCTGGATTTTTGCCATGTTACCCCAGAAGGCCTGCGG
CAGCGGGTCAACGTCTATAAACAGCAGGGCAGCTATGCCGCCGCGATCCGGCTACTTAATGATACCATCCCCAGCTTTGA
AGATCTGAAACTGCCACCGATTATCCGCAAGCTGGCGTCGGAGCCCCGGGGCCTGATTCTGATCACCGGCCCCACCGGCA
GCGGGAAATCCACCACTCTGGCCTCGATGATTGACTACATCAACACCAATCGGGCGGTCCATATTTTAACCATTGAAGAC
CCGGTGGAGTACAAGCACGAACACAAGCACAGCATTGTCCATCAGCGCGAAATCGGGGTCGACGCCAGCTCCTTTGCCTC
AGCCCTGCGGTCTTCATTGCGGGAGGATCCGGACGTCATTCTGGTCGGCGAAATGCGGGACTACGAAACCGTTTCCGCCG
CCGTCACCGCCGCTGAAACCGGCCATCTGGTCTTATCCACCCTTCATACCATCGGCGCGGCCAATACGATTGACCGAATC
ATTGACGTCTTTCCGCCCCACAGCCAGCAGCAGATCCGCACCCAGCTGTCCAGCATTTTAAAAGGGGTCGTCACCCAGCA
GCTGGTGCCGCTAGCCAGCGGCGGTGGCCGGATGGCCGCCCTGGAGGTACTGATCGGCACCGATGCGGTGCTGAATCTGA
TCCGCGAAAACAAAACCCACCAACTGGCCTCAGTGATGCAAACCGGTGGCCGCGATGGCATGAAAACTCTGAATGCTGAT
CTGGCGATGCTGGTCCGGGACCAGAAAATCACCTACGAGGTCGGCCTCGAATGGGCGAGTGATAAGCAGGAGTTTAATCA
GTATTTTGGGCGATAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(14-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.716

99.43

0.504
  pilT Neisseria meningitidis 8013

47.076

97.436

0.459
  pilT Legionella pneumophila strain ERS1305867

46.647

97.721

0.456
  pilT Legionella pneumophila strain Lp02

46.647

97.721

0.456
  pilT Neisseria gonorrhoeae MS11

46.491

97.436

0.453
  pilT Acinetobacter baylyi ADP1

45.19

97.721

0.442
  pilT Pseudomonas aeruginosa PAK

44.898

97.721

0.439
  pilT Pseudomonas stutzeri DSM 10701

44.868

97.151

0.436
  pilT Acinetobacter baumannii strain A118

44.315

97.721

0.433
  pilT Acinetobacter nosocomialis M2

44.315

97.721

0.433
  pilT Acinetobacter baumannii D1279779

44.315

97.721

0.433
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

45.511

92.023

0.419
  pilT Vibrio cholerae strain A1552

45.511

92.023

0.419
  pilU Acinetobacter baylyi ADP1

39.645

96.296

0.382
  pilU Pseudomonas stutzeri DSM 10701

40.994

91.738

0.376
  pilU Vibrio cholerae strain A1552

39.506

92.308

0.365

Multiple sequence alignment