Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   U3C44_RS18290 Genome accession   NZ_CP174016
Coordinates   3840695..3841675 (-) Length   326 a.a.
NCBI ID   WP_032660053.1    Uniprot ID   -
Organism   Enterobacter asburiae strain 2023CK-01510     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3835695..3846675
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  U3C44_RS18260 (U3C44_18270) - 3836571..3837068 (+) 498 WP_029739221.1 SprT family zinc-dependent metalloprotease -
  U3C44_RS18265 (U3C44_18275) endA 3837163..3837870 (+) 708 WP_023333343.1 deoxyribonuclease I -
  U3C44_RS18270 (U3C44_18280) rsmE 3837922..3838653 (+) 732 WP_010435451.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  U3C44_RS18275 (U3C44_18285) gshB 3838673..3839620 (+) 948 WP_032660059.1 glutathione synthase -
  U3C44_RS18280 (U3C44_18290) - 3839708..3840268 (+) 561 WP_032660056.1 YqgE/AlgH family protein -
  U3C44_RS18285 (U3C44_18295) ruvX 3840268..3840684 (+) 417 WP_029739223.1 Holliday junction resolvase RuvX -
  U3C44_RS18290 (U3C44_18300) pilT 3840695..3841675 (-) 981 WP_032660053.1 type IV pilus twitching motility protein PilT Machinery gene
  U3C44_RS18295 (U3C44_18305) - 3841693..3842394 (+) 702 WP_032660051.1 YggS family pyridoxal phosphate-dependent enzyme -
  U3C44_RS18300 (U3C44_18310) - 3842416..3842982 (+) 567 WP_024906554.1 YggT family protein -
  U3C44_RS18305 (U3C44_18315) yggU 3842979..3843275 (+) 297 WP_023333349.1 DUF167 family protein YggU -
  U3C44_RS18310 (U3C44_18320) - 3843279..3843872 (+) 594 WP_032660047.1 XTP/dITP diphosphatase -
  U3C44_RS18315 (U3C44_18325) hemW 3843865..3845007 (+) 1143 WP_032660044.1 radical SAM family heme chaperone HemW -
  U3C44_RS18320 (U3C44_18330) - 3845071..3845409 (+) 339 WP_032660042.1 endonuclease domain-containing protein -
  U3C44_RS18325 (U3C44_18335) - 3845492..3846208 (-) 717 WP_023309135.1 DUF2884 domain-containing protein -
  U3C44_RS18330 (U3C44_18340) - 3846265..3846591 (-) 327 WP_014885198.1 YggL family protein -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35523.50 Da        Isoelectric Point: 6.9108

>NTDB_id=1074095 U3C44_RS18290 WP_032660053.1 3840695..3841675(-) (pilT) [Enterobacter asburiae strain 2023CK-01510]
MDVEEIVALSVKHNVSDLHLCSDSPPRWRRLGRLEPAPFPPPDVGALLKAWLNDEQQGTWWAKGQVDFAATATGGQRLRG
SAFKQMRGVSVTLRLLPRSCPQLSSLGAPRVIPELLSNDAGLILVTGATGSGKSTTLAAMVDFLNHHADGHILTLEDPVE
FIYQSERCLIQQREIGQHSPSFAEALRSALRQDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGASQAIERLVDT
FPAQEKDPVRNQLAGSLRAVLAQRLLPDLQGGRVALYELLVNTAAAANLIREGKTWQLPGIIQTGQQAGMQNFDQSLSER
RAQGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=1074095 U3C44_RS18290 WP_032660053.1 3840695..3841675(-) (pilT) [Enterobacter asburiae strain 2023CK-01510]
ATGGATGTGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAACGTCTCCGATCTACACCTGTGCAGTGATTCACCGCCGCG
CTGGCGCAGATTAGGCCGGCTTGAACCCGCTCCCTTTCCGCCTCCCGATGTCGGAGCGTTATTAAAAGCGTGGCTCAACG
ATGAGCAGCAGGGGACATGGTGGGCAAAGGGCCAGGTGGATTTTGCCGCGACGGCGACGGGAGGCCAGCGGCTGCGCGGC
AGTGCCTTCAAGCAGATGAGAGGTGTCTCTGTGACGCTGCGGCTGTTGCCGCGTAGCTGCCCGCAACTCTCTTCGCTGGG
CGCGCCGAGGGTAATCCCGGAACTGTTATCCAATGACGCCGGGCTGATTCTGGTCACGGGGGCGACCGGCAGCGGCAAAT
CCACTACGCTGGCGGCGATGGTCGATTTTCTTAACCACCATGCTGACGGCCATATCCTCACGCTTGAAGATCCGGTGGAG
TTTATCTACCAGAGCGAACGTTGCCTGATCCAGCAGCGGGAGATAGGCCAGCACAGCCCGTCATTTGCTGAAGCGCTGCG
CAGCGCCTTACGCCAGGATCCGGACGTTATTCTGCTGGGAGAACTGCGCGACAGCGAAACGATCCGCCTGGCGCTGACGG
CGGCGGAAACCGGACATCTGGTGCTGGCGACGCTGCATACGCGCGGGGCATCGCAGGCGATTGAACGGCTGGTCGATACG
TTCCCGGCGCAGGAGAAAGATCCGGTGCGTAACCAGCTGGCCGGCAGCCTGCGGGCGGTGCTGGCGCAGAGGCTGCTTCC
CGATCTACAGGGCGGGCGCGTCGCGTTGTATGAACTGCTGGTGAACACAGCGGCGGCGGCGAATCTGATTCGCGAAGGGA
AAACGTGGCAACTGCCCGGCATCATTCAAACCGGTCAGCAGGCAGGAATGCAGAACTTTGACCAGAGCCTGTCGGAGAGA
CGGGCGCAGGGGCGGCTGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(74-266)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50.459

100

0.506
  pilT Vibrio cholerae strain A1552

50.459

100

0.506
  pilT Pseudomonas stutzeri DSM 10701

48.624

100

0.488
  pilT Legionella pneumophila strain ERS1305867

49.379

98.773

0.488
  pilT Legionella pneumophila strain Lp02

49.379

98.773

0.488
  pilT Acinetobacter baylyi ADP1

48.012

100

0.482
  pilT Acinetobacter baumannii D1279779

48.012

100

0.482
  pilT Acinetobacter baumannii strain A118

48.012

100

0.482
  pilT Neisseria meningitidis 8013

47.561

100

0.479
  pilT Pseudomonas aeruginosa PAK

47.706

100

0.479
  pilT Acinetobacter nosocomialis M2

48.287

98.466

0.475
  pilT Neisseria gonorrhoeae MS11

47.256

100

0.475
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.168

98.773

0.426
  pilU Pseudomonas stutzeri DSM 10701

40

100

0.405
  pilU Vibrio cholerae strain A1552

39.752

98.773

0.393
  pilU Acinetobacter baylyi ADP1

36.646

98.773

0.362

Multiple sequence alignment