Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   ACIRUI_RS03915 Genome accession   NZ_CP173697
Coordinates   777806..778867 (-) Length   353 a.a.
NCBI ID   WP_172679141.1    Uniprot ID   -
Organism   Roseburia faecis strain JCM 17581     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 772806..783867
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACIRUI_RS03895 (ACIRUI_03900) pilM 773196..774962 (-) 1767 WP_055067746.1 pilus assembly protein PilM -
  ACIRUI_RS03900 (ACIRUI_03905) - 775203..775967 (-) 765 WP_022046080.1 A24 family peptidase -
  ACIRUI_RS03905 (ACIRUI_03910) - 775997..776488 (-) 492 WP_055067745.1 type II secretion system protein -
  ACIRUI_RS03910 (ACIRUI_03915) - 776580..777791 (-) 1212 WP_055067744.1 type II secretion system F family protein -
  ACIRUI_RS03915 (ACIRUI_03920) pilT 777806..778867 (-) 1062 WP_172679141.1 type IV pilus twitching motility protein PilT Machinery gene
  ACIRUI_RS03920 (ACIRUI_03925) - 778899..780644 (-) 1746 WP_055067743.1 GspE/PulE family protein -
  ACIRUI_RS03925 (ACIRUI_03930) spoIVB 780803..782095 (-) 1293 WP_055067742.1 SpoIVB peptidase -
  ACIRUI_RS03930 (ACIRUI_03935) recN 782151..783830 (-) 1680 WP_022046133.1 DNA repair protein RecN -

Sequence

Protein


Download         Length: 353 a.a.        Molecular weight: 38906.64 Da        Isoelectric Point: 6.6404

>NTDB_id=1073761 ACIRUI_RS03915 WP_172679141.1 777806..778867(-) (pilT) [Roseburia faecis strain JCM 17581]
MELSMYTLLSIAKAHHASDLHITVGVRPKCRISGTLYEMDGFDKLTPAMTKELVESMFGPKQKATMEATGEVDFAYTDTR
VGRFRVNAFHQRGSYAAVLRIVASDIPTPEDLGVPEAVLGLTKKKRGLVLVTGPTGSGKSTTLASLIDVINKERNEHIIT
LEDPIEYLHKHNRSIVNQREIGLDTDSYGNALRAALREDPDIILVGEMRDFDTISTAITAAETGHLVFSTLHTIGAAPTI
ERIIDVFPPHQQNQIRSQLASVLEAVISQQLLPTIDGKSRVAAFEVMLGTPAIKNLIREDKAHQIPSIMQTSKKIGMQTM
DDSLFSLYLKHKIDAANAVEYAQDTGNMQQKLF

Nucleotide


Download         Length: 1062 bp        

>NTDB_id=1073761 ACIRUI_RS03915 WP_172679141.1 777806..778867(-) (pilT) [Roseburia faecis strain JCM 17581]
ATGGAATTGTCAATGTATACGCTTCTTTCGATAGCAAAAGCGCATCATGCGTCCGACTTACATATTACAGTTGGGGTTCG
CCCAAAATGCCGTATTAGCGGAACACTTTACGAGATGGATGGTTTTGATAAGCTGACACCTGCGATGACAAAAGAATTGG
TGGAATCTATGTTTGGACCGAAACAGAAGGCAACCATGGAGGCCACAGGGGAAGTGGATTTTGCCTACACGGATACGCGT
GTGGGACGGTTCCGTGTCAACGCTTTCCACCAGAGAGGTTCCTATGCGGCTGTACTGCGTATCGTGGCAAGCGATATCCC
GACACCGGAGGACCTCGGGGTGCCGGAAGCTGTCCTTGGGCTGACAAAGAAAAAGCGTGGGCTTGTCCTTGTCACGGGAC
CGACCGGAAGCGGTAAATCCACCACACTGGCATCTTTGATCGATGTCATCAATAAAGAACGGAACGAACATATCATTACA
CTCGAAGATCCGATCGAGTATTTACATAAGCATAACCGGTCCATCGTAAACCAAAGGGAGATCGGGCTGGATACCGATTC
CTATGGAAATGCGCTCCGTGCGGCGCTCCGTGAGGATCCGGACATCATCCTTGTGGGGGAGATGCGTGACTTTGATACGA
TTTCCACTGCCATCACAGCAGCAGAAACCGGGCATCTGGTATTCTCCACCCTGCATACCATCGGTGCGGCACCTACCATT
GAGCGTATTATCGATGTGTTCCCACCGCACCAGCAGAACCAGATCCGCTCGCAGCTGGCATCGGTTCTTGAGGCGGTAAT
TTCCCAGCAGCTTCTGCCGACGATCGACGGAAAATCCCGTGTGGCGGCATTTGAAGTCATGCTCGGTACCCCGGCGATCA
AAAACCTGATCCGGGAGGATAAGGCACACCAGATTCCATCCATCATGCAGACAAGTAAGAAGATTGGCATGCAGACGATG
GATGATTCCTTATTCAGCCTGTATCTGAAACACAAAATCGATGCGGCAAATGCCGTAGAGTATGCACAGGATACAGGAAA
CATGCAGCAGAAGCTGTTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(116-272)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.445

95.467

0.482
  pilT Pseudomonas stutzeri DSM 10701

49.275

97.734

0.482
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50.751

94.334

0.479
  pilT Vibrio cholerae strain A1552

50.751

94.334

0.479
  pilT Legionella pneumophila strain ERS1305867

49.704

95.751

0.476
  pilT Legionella pneumophila strain Lp02

49.704

95.751

0.476
  pilT Pseudomonas aeruginosa PAK

49.263

96.034

0.473
  pilT Neisseria meningitidis 8013

48.116

97.734

0.47
  pilT Neisseria gonorrhoeae MS11

47.826

97.734

0.467
  pilT Acinetobacter baylyi ADP1

47.941

96.317

0.462
  pilT Acinetobacter baumannii D1279779

48.64

93.768

0.456
  pilT Acinetobacter baumannii strain A118

48.64

93.768

0.456
  pilT Acinetobacter nosocomialis M2

49.688

90.652

0.45
  pilU Vibrio cholerae strain A1552

43.266

98.867

0.428
  pilU Acinetobacter baylyi ADP1

43.44

97.167

0.422
  pilU Pseudomonas stutzeri DSM 10701

41.813

96.884

0.405

Multiple sequence alignment