Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   ACI516_RS17525 Genome accession   NZ_CP173625
Coordinates   3868925..3869941 (-) Length   338 a.a.
NCBI ID   WP_335473087.1    Uniprot ID   -
Organism   Pectobacterium carotovorum strain M8a     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3863925..3874941
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACI516_RS17510 (ACI516_17505) nsrR 3864298..3864723 (-) 426 WP_010279087.1 nitric oxide-sensing transcriptional repressor NsrR -
  ACI516_RS17515 (ACI516_17510) - 3865019..3866590 (+) 1572 WP_039545636.1 methyl-accepting chemotaxis protein -
  ACI516_RS17520 (ACI516_17515) - 3867124..3868956 (+) 1833 WP_405078471.1 diguanylate cyclase -
  ACI516_RS17525 (ACI516_17520) pilT 3868925..3869941 (-) 1017 WP_335473087.1 type IV pilus twitching motility protein PilT Machinery gene
  ACI516_RS17530 (ACI516_17525) - 3869963..3870676 (+) 714 WP_161509782.1 YggS family pyridoxal phosphate-dependent enzyme -
  ACI516_RS17535 (ACI516_17530) proC 3870774..3871595 (+) 822 WP_180775873.1 pyrroline-5-carboxylate reductase -
  ACI516_RS17540 (ACI516_17535) - 3871645..3872199 (+) 555 WP_010295003.1 YggT family protein -
  ACI516_RS17545 (ACI516_17540) yggU 3872196..3872486 (+) 291 WP_010295005.1 DUF167 family protein YggU -
  ACI516_RS17550 (ACI516_17545) - 3872518..3873111 (+) 594 WP_039470191.1 XTP/dITP diphosphatase -
  ACI516_RS17555 (ACI516_17550) hemW 3873104..3874246 (+) 1143 WP_039530837.1 radical SAM family heme chaperone HemW -
  ACI516_RS17560 (ACI516_17555) - 3874472..3874735 (+) 264 WP_226293727.1 type II toxin-antitoxin system ParD family antitoxin -

Sequence

Protein


Download         Length: 338 a.a.        Molecular weight: 36779.08 Da        Isoelectric Point: 6.5647

>NTDB_id=1073310 ACI516_RS17525 WP_335473087.1 3868925..3869941(-) (pilT) [Pectobacterium carotovorum strain M8a]
MELDEWMARSVKHNASDLHLCSGHPPVLRVDGRLQPENTLPRLTPDQVAQWCDAWLEPAQCEQLRQAGQVDGALMLPDGQ
RLRVNVFRQREGLSAALRVIPSVQPSLDALHAPPVFSTLLEKPNGLILITGATGSGKSTTLAAMLGALNERCDRHVITLE
DPIEFIHASRRCLIQQREIGAHSASFAQALRAALREDPDVILLGELRDTETIRLALTAAETGHLVLSTLHTRSASQAVDR
LIDVFPGEEKAYVRSQLATCLQAVVTQKLLPAAQGGRIALYEVLTATAAVSNLIREGKTHQLPGLIQTGAAAGMQTFEQS
YQQRCRDGLISHSCDLAV

Nucleotide


Download         Length: 1017 bp        

>NTDB_id=1073310 ACI516_RS17525 WP_335473087.1 3868925..3869941(-) (pilT) [Pectobacterium carotovorum strain M8a]
ATGGAATTGGATGAGTGGATGGCGCGTAGTGTAAAACATAATGCCTCGGATCTGCACCTTTGTAGCGGTCATCCGCCGGT
GTTACGTGTGGACGGGCGGCTACAGCCTGAAAATACCTTACCACGTTTAACACCGGATCAGGTGGCACAATGGTGCGATG
CCTGGCTTGAACCGGCTCAGTGCGAGCAACTGCGGCAGGCTGGTCAGGTGGACGGGGCATTAATGCTGCCCGATGGACAA
CGCCTGCGGGTGAATGTGTTTCGCCAGCGGGAAGGGCTGTCTGCGGCGTTGCGTGTTATCCCGTCCGTTCAACCTTCATT
GGATGCGCTACATGCGCCACCCGTTTTCTCGACGCTGCTGGAGAAACCGAACGGACTGATCCTGATTACCGGGGCGACGG
GGAGCGGCAAATCCACGACGCTGGCGGCCATGCTTGGGGCGTTAAACGAGCGCTGCGATCGCCATGTGATTACGCTGGAG
GATCCGATCGAGTTTATCCATGCCAGCCGACGCTGCCTGATTCAACAGCGAGAGATCGGTGCGCATAGCGCGTCATTTGC
GCAGGCACTACGGGCCGCATTGCGGGAGGATCCCGATGTCATTCTGCTGGGAGAACTGCGCGATACGGAGACGATTCGGC
TGGCGCTGACGGCGGCGGAAACCGGCCATCTGGTGTTGTCGACGCTGCATACCCGTAGCGCATCGCAGGCGGTCGATCGT
TTGATTGACGTTTTTCCCGGTGAAGAGAAAGCCTATGTTCGCAGCCAGTTAGCCACCTGTTTGCAGGCAGTGGTGACGCA
AAAGCTGTTGCCTGCGGCGCAAGGTGGCCGCATTGCGCTTTATGAAGTGCTGACCGCCACCGCAGCAGTCAGTAACCTGA
TTCGGGAAGGGAAAACGCACCAGCTCCCCGGCCTGATTCAGACGGGAGCCGCTGCGGGAATGCAAACGTTCGAGCAAAGC
TACCAGCAGCGCTGTCGGGATGGGCTTATTTCACACAGTTGCGACCTTGCCGTTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

51.818

97.633

0.506
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

51.818

97.633

0.506
  pilT Neisseria meningitidis 8013

50.602

98.225

0.497
  pilT Neisseria gonorrhoeae MS11

50.301

98.225

0.494
  pilT Pseudomonas stutzeri DSM 10701

49.245

97.929

0.482
  pilT Acinetobacter nosocomialis M2

48.943

97.929

0.479
  pilT Acinetobacter baylyi ADP1

48.943

97.929

0.479
  pilT Acinetobacter baumannii D1279779

48.943

97.929

0.479
  pilT Acinetobacter baumannii strain A118

48.943

97.929

0.479
  pilT Pseudomonas aeruginosa PAK

48.943

97.929

0.479
  pilT Legionella pneumophila strain ERS1305867

48.64

97.929

0.476
  pilT Legionella pneumophila strain Lp02

48.64

97.929

0.476
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.136

99.704

0.42
  pilU Vibrio cholerae strain A1552

38.776

100

0.393
  pilU Pseudomonas stutzeri DSM 10701

39.039

98.521

0.385
  pilU Acinetobacter baylyi ADP1

38.199

95.266

0.364

Multiple sequence alignment