Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   ACI514_RS27375 Genome accession   NZ_CP173611
Coordinates   6152157..6153191 (+) Length   344 a.a.
NCBI ID   WP_007913646.1    Uniprot ID   J3FC36
Organism   Pseudomonas sp. M20     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 6147157..6158191
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACI514_RS27345 (ACI514_27330) metW 6147580..6148200 (-) 621 WP_160059761.1 methionine biosynthesis protein MetW -
  ACI514_RS27350 (ACI514_27335) - 6148208..6149347 (-) 1140 WP_123593598.1 homoserine O-acetyltransferase -
  ACI514_RS27355 (ACI514_27340) - 6149678..6149974 (-) 297 WP_405122716.1 DUF167 domain-containing protein -
  ACI514_RS27360 (ACI514_27345) - 6149971..6150561 (-) 591 WP_038358826.1 YggT family protein -
  ACI514_RS27365 (ACI514_27350) proC 6150572..6151390 (-) 819 WP_160059759.1 pyrroline-5-carboxylate reductase -
  ACI514_RS27370 (ACI514_27355) - 6151414..6152100 (-) 687 WP_160059758.1 YggS family pyridoxal phosphate-dependent enzyme -
  ACI514_RS27375 (ACI514_27360) pilT 6152157..6153191 (+) 1035 WP_007913646.1 type IV pilus twitching motility protein PilT Machinery gene
  ACI514_RS27380 (ACI514_27365) - 6153297..6153914 (-) 618 WP_016985978.1 C40 family peptidase -
  ACI514_RS27385 (ACI514_27370) - 6154163..6154594 (+) 432 WP_122596578.1 TM2 domain-containing protein -
  ACI514_RS27390 (ACI514_27375) - 6155112..6156098 (-) 987 WP_405122717.1 lipid II-degrading bacteriocin -
  ACI514_RS27395 (ACI514_27380) - 6156420..6157691 (-) 1272 WP_405122718.1 dihydroorotase -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 38041.71 Da        Isoelectric Point: 7.0157

>NTDB_id=1073165 ACI514_RS27375 WP_007913646.1 6152157..6153191(+) (pilT) [Pseudomonas sp. M20]
MDITELLAFSAKQGASDLHLSAGLPPMIRVDGDVRRINLPALDHKQVHELIYDIMNDTQRVDFEKHLETDFSFEVPGVAR
FRVNAFNQNRGAGAVFRTIPSKVLSMEDLAMGDVFRKITDAPRGLVLVTGPTGSGKSTTLAAMIDYLNTHRHHHILTIED
PIEFVHESRKCLINQREVHRDTRSFATALRSALREDPDVILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRV
VDVFPGDEKSMVRSMLSESLLAVVSQTLIKKIGGGRVAAHEIMLGTSAIRNLIREDKVAQMYSAIQTGGSLGMQTLDMCL
KDLVAKGLISREHAREKARTPDNF

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=1073165 ACI514_RS27375 WP_007913646.1 6152157..6153191(+) (pilT) [Pseudomonas sp. M20]
ATGGATATCACTGAACTGCTGGCCTTCAGCGCCAAACAAGGCGCTTCCGACCTGCACCTGTCGGCCGGTCTGCCGCCGAT
GATCCGTGTCGATGGCGATGTGCGGCGGATCAATCTGCCGGCGCTGGATCACAAGCAAGTGCACGAATTGATCTACGACA
TCATGAACGACACCCAGCGGGTCGACTTCGAGAAACACCTGGAAACCGACTTTTCCTTCGAAGTCCCCGGTGTCGCGCGC
TTTCGGGTCAATGCCTTCAACCAGAACCGTGGCGCCGGCGCTGTATTCCGTACGATTCCGTCGAAAGTGCTGAGCATGGA
AGACCTCGCCATGGGCGACGTCTTCCGAAAGATCACCGATGCCCCGCGCGGGCTGGTGCTGGTCACCGGGCCGACCGGTT
CGGGAAAATCGACCACACTGGCGGCGATGATCGATTACCTCAACACCCACCGTCATCACCATATCCTGACTATTGAAGAC
CCGATCGAGTTTGTTCACGAATCGCGCAAATGCCTGATCAATCAGCGCGAAGTCCATCGTGATACCCGCAGTTTCGCCAC
CGCTCTGCGCTCGGCGTTGCGCGAAGACCCGGACGTGATTCTGGTCGGCGAGATGCGTGACCTGGAAACCATTCGCCTGG
CGCTGACCGCCGCCGAAACCGGGCATCTGGTGTTCGGCACGCTGCACACCACGTCAGCGGCGAAAACCATCGACCGTGTG
GTCGACGTATTCCCGGGTGACGAGAAGTCGATGGTGCGCTCGATGCTGTCGGAGTCGTTGCTGGCGGTGGTCTCGCAGAC
GCTGATCAAGAAGATCGGCGGCGGGCGGGTGGCGGCGCACGAGATCATGCTGGGCACGTCGGCGATCCGTAACCTGATCC
GCGAGGACAAGGTCGCGCAGATGTATTCGGCGATTCAAACGGGTGGGTCGTTGGGGATGCAGACACTGGACATGTGCCTG
AAAGATCTGGTGGCCAAGGGCTTGATCAGCCGTGAGCATGCGCGGGAGAAGGCGCGTACGCCGGATAATTTCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB J3FC36

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas stutzeri DSM 10701

88.081

100

0.881
  pilT Pseudomonas aeruginosa PAK

88.081

100

0.881
  pilT Acinetobacter baumannii D1279779

79.942

100

0.799
  pilT Acinetobacter baumannii strain A118

79.942

100

0.799
  pilT Acinetobacter nosocomialis M2

79.651

100

0.797
  pilT Acinetobacter baylyi ADP1

77.907

100

0.779
  pilT Legionella pneumophila strain Lp02

72.485

98.256

0.712
  pilT Legionella pneumophila strain ERS1305867

72.485

98.256

0.712
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

68.843

97.965

0.674
  pilT Vibrio cholerae strain A1552

68.843

97.965

0.674
  pilT Neisseria meningitidis 8013

66.377

100

0.666
  pilT Neisseria gonorrhoeae MS11

66.087

100

0.663
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.594

100

0.517
  pilU Acinetobacter baylyi ADP1

40.462

100

0.407
  pilU Pseudomonas stutzeri DSM 10701

41.493

97.384

0.404
  pilU Vibrio cholerae strain A1552

39.71

100

0.398

Multiple sequence alignment