Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   ACI511_RS04030 Genome accession   NZ_CP173610
Coordinates   916782..917798 (+) Length   338 a.a.
NCBI ID   WP_125233078.1    Uniprot ID   -
Organism   Pectobacterium versatile strain M22b     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 911782..922798
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACI511_RS04000 (ACI511_04005) hemW 912473..913615 (-) 1143 WP_107333418.1 radical SAM family heme chaperone HemW -
  ACI511_RS04005 (ACI511_04010) - 913608..914201 (-) 594 WP_405078004.1 XTP/dITP diphosphatase -
  ACI511_RS04010 (ACI511_04015) yggU 914233..914523 (-) 291 WP_010295005.1 DUF167 family protein YggU -
  ACI511_RS04015 (ACI511_04020) - 914520..915074 (-) 555 WP_010295003.1 YggT family protein -
  ACI511_RS04020 (ACI511_04025) proC 915124..915945 (-) 822 WP_212769123.1 pyrroline-5-carboxylate reductase -
  ACI511_RS04025 (ACI511_04030) - 916047..916760 (-) 714 WP_125233079.1 YggS family pyridoxal phosphate-dependent enzyme -
  ACI511_RS04030 (ACI511_04035) pilT 916782..917798 (+) 1017 WP_125233078.1 type IV pilus twitching motility protein PilT Machinery gene
  ACI511_RS04035 (ACI511_04040) - 917767..919599 (-) 1833 WP_125233077.1 diguanylate cyclase -
  ACI511_RS04040 (ACI511_04045) - 920133..921704 (-) 1572 WP_211005678.1 methyl-accepting chemotaxis protein -
  ACI511_RS04045 (ACI511_04050) nsrR 922048..922473 (+) 426 WP_010279087.1 nitric oxide-sensing transcriptional repressor NsrR -

Sequence

Protein


Download         Length: 338 a.a.        Molecular weight: 36787.99 Da        Isoelectric Point: 6.6280

>NTDB_id=1073128 ACI511_RS04030 WP_125233078.1 916782..917798(+) (pilT) [Pectobacterium versatile strain M22b]
MELDEWMARSVKHNASDLHLCSGHPPVLRVDGRLQPENTLPRLTPDQVAQWCDAWLEPAQREQLRQAGQVDGALMLPDGQ
RLRVNVFRQREGLSAALRVIPSAQPSLDALHAPPVFSTLLEKPNGLLLITGATGSGKSTTLAAMLGALNDRCDRHVITLE
DPIEFIHASRRCLIQQREIGAHSASFSQALRAALREDPDVILLGELRDTETIRLALTAAETGHLVLSTLHTRSASQAVDR
LIDVFPGEEKAYVRSQLATCLQAVVTQTLLPAAHGGRIALYEVLTATAAVSNLIREGKTHQLPGLIQTGAAAGMQTFEQS
YQQRCRDGLISHSCDLAV

Nucleotide


Download         Length: 1017 bp        

>NTDB_id=1073128 ACI511_RS04030 WP_125233078.1 916782..917798(+) (pilT) [Pectobacterium versatile strain M22b]
ATGGAGTTGGATGAGTGGATGGCGCGTAGTGTAAAACATAATGCCTCGGATCTGCACCTTTGTAGCGGTCACCCGCCGGT
GTTGCGTGTGGACGGGCGGCTACAGCCTGAAAATACCTTACCACGTTTAACGCCGGATCAGGTGGCACAATGGTGCGATG
CCTGGCTCGAACCGGCTCAGCGCGAGCAACTGCGGCAGGCTGGTCAGGTGGATGGGGCATTAATGCTGCCCGATGGACAG
CGCCTGCGGGTGAACGTGTTTCGCCAGCGTGAGGGGCTGTCTGCGGCGCTGCGCGTTATTCCATCCGCTCAGCCTTCATT
GGACGCGCTACACGCGCCACCCGTTTTCTCGACGCTGCTGGAGAAACCGAACGGACTGCTCCTGATTACCGGTGCGACGG
GGAGCGGCAAATCCACAACGCTGGCGGCCATGCTTGGGGCGTTAAACGACCGCTGCGATCGCCATGTGATTACGCTGGAG
GATCCGATCGAGTTTATCCATGCCAGCCGACGCTGCCTGATTCAACAGCGGGAGATCGGTGCGCACAGCGCGTCGTTTTC
GCAGGCATTACGGGCCGCATTGCGGGAGGATCCCGATGTCATTCTGCTGGGAGAACTACGCGATACGGAGACGATTCGGC
TGGCGCTGACGGCGGCGGAAACCGGCCATCTGGTGTTGTCGACGCTGCATACCCGTAGCGCATCGCAGGCGGTCGATCGT
CTGATTGACGTCTTTCCTGGTGAAGAAAAAGCCTATGTTCGCAGCCAGTTAGCCACCTGTTTGCAGGCGGTGGTGACGCA
AACACTCTTGCCTGCGGCACACGGTGGCCGCATTGCGCTTTATGAAGTACTGACCGCCACCGCGGCAGTCAGCAACCTGA
TTCGGGAAGGGAAAACGCACCAGCTCCCCGGCCTGATTCAGACGGGAGCCGCTGCGGGAATGCAAACGTTCGAGCAAAGC
TACCAGCAGCGCTGTCGGGATGGGCTTATTTCACACAGTTGCGACCTTGCCGTTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-262)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

51.515

97.633

0.503
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

51.515

97.633

0.503
  pilT Neisseria meningitidis 8013

50.301

98.225

0.494
  pilT Neisseria gonorrhoeae MS11

50

98.225

0.491
  pilT Pseudomonas stutzeri DSM 10701

50.151

97.929

0.491
  pilT Pseudomonas aeruginosa PAK

49.849

97.929

0.488
  pilT Acinetobacter baylyi ADP1

49.547

97.929

0.485
  pilT Acinetobacter baumannii strain A118

49.245

97.929

0.482
  pilT Acinetobacter nosocomialis M2

49.245

97.929

0.482
  pilT Acinetobacter baumannii D1279779

49.245

97.929

0.482
  pilT Legionella pneumophila strain ERS1305867

48.943

97.929

0.479
  pilT Legionella pneumophila strain Lp02

48.943

97.929

0.479
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.136

99.704

0.42
  pilU Vibrio cholerae strain A1552

38.776

100

0.393
  pilU Pseudomonas stutzeri DSM 10701

39.339

98.521

0.388
  pilU Acinetobacter baylyi ADP1

38.199

95.266

0.364

Multiple sequence alignment