Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   AACH44_RS16435 Genome accession   NZ_AP028908
Coordinates   3634622..3635638 (-) Length   338 a.a.
NCBI ID   WP_338659328.1    Uniprot ID   A0AAN0MM76
Organism   Pectobacterium araliae strain MAFF 302110     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3629622..3640638
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AACH44_RS16420 (PEC302110_32110) nsrR 3630019..3630444 (-) 426 WP_261846976.1 nitric oxide-sensing transcriptional repressor NsrR -
  AACH44_RS16425 (PEC302110_32120) - 3630739..3632310 (+) 1572 WP_261846977.1 methyl-accepting chemotaxis protein -
  AACH44_RS16430 (PEC302110_32130) - 3632821..3634653 (+) 1833 WP_261846978.1 diguanylate cyclase -
  AACH44_RS16435 (PEC302110_32140) pilT 3634622..3635638 (-) 1017 WP_338659328.1 type IV pilus twitching motility protein PilT Machinery gene
  AACH44_RS16440 (PEC302110_32150) - 3635660..3636373 (+) 714 WP_338659329.1 YggS family pyridoxal phosphate-dependent enzyme -
  AACH44_RS16445 (PEC302110_32160) proC 3636460..3637281 (+) 822 WP_338659330.1 pyrroline-5-carboxylate reductase -
  AACH44_RS16450 (PEC302110_32170) - 3637330..3637884 (+) 555 WP_261846982.1 YggT family protein -
  AACH44_RS16455 (PEC302110_32180) yggU 3637881..3638171 (+) 291 WP_261846983.1 DUF167 family protein YggU -
  AACH44_RS16460 (PEC302110_32190) rdgB 3638203..3638796 (+) 594 WP_338659331.1 RdgB/HAM1 family non-canonical purine NTP pyrophosphatase -
  AACH44_RS16465 (PEC302110_32200) hemW 3638789..3639928 (+) 1140 WP_338659332.1 radical SAM family heme chaperone HemW -
  AACH44_RS16470 (PEC302110_32210) - 3640157..3640420 (+) 264 WP_261846986.1 type II toxin-antitoxin system ParD family antitoxin -
  AACH44_RS16475 (PEC302110_32220) - 3640371..3640622 (+) 252 WP_338659635.1 hypothetical protein -

Sequence

Protein


Download         Length: 338 a.a.        Molecular weight: 37015.46 Da        Isoelectric Point: 6.4954

>NTDB_id=107286 AACH44_RS16435 WP_338659328.1 3634622..3635638(-) (pilT) [Pectobacterium araliae strain MAFF 302110]
MELDEWMARSVKHNASDLHLCSGHPPVLRVDGRLQPENTLPCLTPEQVAQWCDAWLEPTQKEQLRQAGQVDGALMLPDGQ
RLRVNVFRQREGLSVALRIIPFVQPSLDELHAPPILATLLEKPDGLILITGATGSGKSTTLAAMVGTLNDSSDRHVITLE
DPIEFIHASRRCLIQQREIGAHSVSFAQALRAALREDPDVILLGELRDTETIRLALTAAETGHLVLSTLHTRRASQAVDR
LIDVFPSEEKAYVRSQLATCLQAVVTQKLLPAAHGGRIALYEVLTVTAAVSNLIREGKTHQLPGLIQTGTAVGMQTFEQS
YQQRCRDGLISHSCALAV

Nucleotide


Download         Length: 1017 bp        

>NTDB_id=107286 AACH44_RS16435 WP_338659328.1 3634622..3635638(-) (pilT) [Pectobacterium araliae strain MAFF 302110]
ATGGAATTGGATGAGTGGATGGCGCGTAGTGTAAAACATAATGCCTCGGATCTGCACCTTTGTAGTGGGCACCCTCCGGT
GTTACGTGTGGATGGGCGGCTACAGCCTGAAAATACCTTACCATGTTTAACGCCGGAACAGGTGGCGCAATGGTGCGATG
CCTGGCTAGAACCGACTCAGAAAGAACAATTGCGGCAGGCAGGGCAGGTGGATGGTGCGTTAATGCTGCCTGATGGGCAG
CGCCTGCGGGTCAATGTGTTTCGCCAGCGGGAAGGGCTGTCCGTGGCGCTGCGTATTATCCCGTTTGTTCAACCTTCGTT
GGATGAATTACATGCGCCACCCATCCTTGCGACGCTGCTGGAGAAACCAGACGGGCTGATCCTGATTACCGGTGCGACGG
GCAGCGGTAAATCCACGACGCTGGCGGCGATGGTTGGAACGTTAAACGACAGCAGCGATCGCCATGTGATCACGTTGGAA
GATCCGATCGAATTTATCCATGCCAGCCGACGATGTCTGATCCAACAGCGAGAGATCGGGGCACACAGCGTGTCTTTTGC
TCAGGCGCTACGAGCAGCATTGCGGGAAGACCCCGATGTTATTCTACTGGGAGAACTGCGCGATACTGAGACGATCCGTC
TGGCGTTGACGGCGGCGGAAACCGGCCATTTGGTACTGTCAACGCTACATACGCGCAGGGCATCGCAGGCCGTTGATCGG
CTCATTGATGTTTTCCCCAGTGAAGAGAAAGCGTATGTTCGCAGCCAGTTAGCTACCTGTTTGCAGGCCGTCGTGACGCA
AAAGTTGTTGCCCGCAGCACACGGTGGCCGAATCGCGCTTTATGAAGTGCTGACGGTGACAGCGGCAGTCAGCAACTTGA
TTCGTGAAGGGAAAACGCACCAGCTTCCCGGCTTGATTCAGACGGGAACGGCTGTGGGAATGCAAACGTTCGAACAAAGC
TATCAGCAGCGCTGTCGGGACGGGCTTATTTCACACAGTTGCGCCCTTGCCGTTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Neisseria meningitidis 8013

49.702

99.408

0.494
  pilT Neisseria gonorrhoeae MS11

49.405

99.408

0.491
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50

97.633

0.488
  pilT Vibrio cholerae strain A1552

50

97.633

0.488
  pilT Acinetobacter baylyi ADP1

47.633

100

0.476
  pilT Legionella pneumophila strain ERS1305867

47.761

99.112

0.473
  pilT Legionella pneumophila strain Lp02

47.761

99.112

0.473
  pilT Pseudomonas stutzeri DSM 10701

47.164

99.112

0.467
  pilT Pseudomonas aeruginosa PAK

47.164

99.112

0.467
  pilT Acinetobacter baumannii D1279779

46.866

99.112

0.465
  pilT Acinetobacter nosocomialis M2

46.866

99.112

0.465
  pilT Acinetobacter baumannii strain A118

46.866

99.112

0.465
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.543

99.704

0.414
  pilU Pseudomonas stutzeri DSM 10701

40.059

99.704

0.399
  pilU Vibrio cholerae strain A1552

38.872

99.704

0.388
  pilU Acinetobacter baylyi ADP1

38.166

100

0.382

Multiple sequence alignment