Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   ACI3KK_RS21135 Genome accession   NZ_CP173421
Coordinates   4399782..4400816 (-) Length   344 a.a.
NCBI ID   WP_136663428.1    Uniprot ID   A0A4V4R8D5
Organism   Pseudomonas leptonychotis strain TMP18     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4394782..4405816
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACI3KK_RS21115 (ACI3KK_21125) - 4395918..4396316 (-) 399 WP_136663424.1 TM2 domain-containing protein -
  ACI3KK_RS21120 (ACI3KK_21130) - 4396475..4397686 (+) 1212 WP_405119269.1 putative DNA modification/repair radical SAM protein -
  ACI3KK_RS21125 (ACI3KK_21135) - 4397686..4398564 (+) 879 WP_405119270.1 TIGR03915 family putative DNA repair protein -
  ACI3KK_RS21130 (ACI3KK_21140) pilU 4398530..4399675 (-) 1146 WP_136663427.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ACI3KK_RS21135 (ACI3KK_21145) pilT 4399782..4400816 (-) 1035 WP_136663428.1 type IV pilus twitching motility protein PilT Machinery gene
  ACI3KK_RS21140 (ACI3KK_21150) - 4400880..4401566 (+) 687 WP_405119271.1 YggS family pyridoxal phosphate-dependent enzyme -
  ACI3KK_RS21145 (ACI3KK_21155) proC 4401595..4402413 (+) 819 WP_136663430.1 pyrroline-5-carboxylate reductase -
  ACI3KK_RS21150 (ACI3KK_21160) - 4402423..4403013 (+) 591 WP_136663431.1 YggT family protein -
  ACI3KK_RS21155 (ACI3KK_21165) - 4403359..4405341 (+) 1983 WP_405119272.1 dynamin family protein -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 37913.67 Da        Isoelectric Point: 6.8361

>NTDB_id=1072199 ACI3KK_RS21135 WP_136663428.1 4399782..4400816(-) (pilT) [Pseudomonas leptonychotis strain TMP18]
MDITELLAFSAKQGASDLHLSAGLPPMIRVDGDVRRINLPALDHKQVHALIYDIMNDKQRKDFEEFLETDFSFEVPGVAR
FRVNAFNQNRGAGAVFRTIPSKVLSMEDLGMGEIFKKISDVPRGLVLVTGPTGSGKSTTLAAMLDYINSNKYHHILTVED
PIEFVHESKKCLINQREVHRDTLGFSEALRSALREDPDIILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRV
VDVFPAEEKSMVRSMLSESLQSVISQTLLKKVGGGRVAAHEIMIGTPAIRNLIREDKVAQMYSAIQTGGSLGMQTLDSCL
KTLLSKGIVSREAAREKAKIPENF

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=1072199 ACI3KK_RS21135 WP_136663428.1 4399782..4400816(-) (pilT) [Pseudomonas leptonychotis strain TMP18]
ATGGATATCACTGAATTGCTGGCCTTCAGCGCCAAGCAAGGTGCATCGGACCTGCACCTCTCCGCTGGCCTGCCTCCGAT
GATTCGGGTCGACGGTGATGTTCGCCGGATCAATTTGCCTGCACTCGACCATAAACAAGTGCACGCGCTGATCTACGACA
TCATGAACGATAAGCAGCGTAAGGATTTCGAGGAGTTCCTCGAGACCGACTTCTCCTTTGAAGTGCCGGGTGTAGCGCGT
TTCCGGGTTAACGCCTTCAACCAGAACCGTGGCGCTGGCGCCGTATTCCGTACCATTCCGTCGAAAGTGCTGAGCATGGA
AGATCTCGGTATGGGCGAGATCTTCAAGAAGATTTCTGATGTGCCGCGTGGTTTGGTGCTGGTCACCGGGCCCACCGGTT
CGGGTAAGTCGACCACCCTGGCGGCGATGCTCGACTATATAAATAGCAATAAGTACCACCACATCCTCACGGTGGAAGAC
CCGATCGAGTTCGTTCACGAATCGAAAAAATGCCTGATTAACCAGCGCGAAGTGCACCGCGACACCCTCGGCTTCTCCGA
AGCCTTGCGCTCGGCGCTGCGTGAAGACCCGGATATCATCCTGGTGGGCGAAATGCGCGACCTGGAAACCATTCGCTTGG
CGCTGACTGCCGCGGAAACGGGTCACTTGGTATTTGGCACTCTGCACACCACCTCAGCGGCTAAAACCATCGACCGGGTG
GTCGACGTATTCCCCGCCGAAGAGAAATCCATGGTGCGCTCCATGCTCTCGGAGTCCCTGCAGTCGGTGATCTCGCAAAC
CCTGTTGAAAAAGGTTGGCGGCGGCCGTGTGGCGGCCCATGAAATCATGATTGGTACGCCGGCTATCCGTAACCTGATCC
GTGAGGACAAGGTGGCGCAGATGTATTCGGCCATTCAAACCGGTGGCTCGCTGGGCATGCAAACCCTCGATTCCTGCCTG
AAGACCCTGTTGTCTAAGGGCATCGTCAGCCGCGAAGCCGCGCGGGAAAAAGCCAAAATTCCGGAAAACTTCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A4V4R8D5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas stutzeri DSM 10701

93.895

100

0.939
  pilT Pseudomonas aeruginosa PAK

93.605

100

0.936
  pilT Acinetobacter nosocomialis M2

85.465

100

0.855
  pilT Acinetobacter baumannii D1279779

85.174

100

0.852
  pilT Acinetobacter baumannii strain A118

85.174

100

0.852
  pilT Acinetobacter baylyi ADP1

83.14

100

0.831
  pilT Legionella pneumophila strain Lp02

75.74

98.256

0.744
  pilT Legionella pneumophila strain ERS1305867

75.74

98.256

0.744
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

68.235

98.837

0.674
  pilT Vibrio cholerae strain A1552

68.235

98.837

0.674
  pilT Neisseria gonorrhoeae MS11

66.957

100

0.672
  pilT Neisseria meningitidis 8013

66.957

100

0.672
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.304

100

0.515
  pilU Acinetobacter baylyi ADP1

39.884

100

0.401
  pilU Vibrio cholerae strain A1552

40.413

98.547

0.398
  pilU Pseudomonas stutzeri DSM 10701

40

97.384

0.39

Multiple sequence alignment