Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   ACIFYW_RS11210 Genome accession   NZ_CP173026
Coordinates   2502271..2503377 (-) Length   368 a.a.
NCBI ID   WP_000422572.1    Uniprot ID   -
Organism   Vibrio cholerae strain SPH 24-01     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2497271..2508377
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACIFYW_RS11185 (ACIFYW_11185) gshB 2497884..2498840 (+) 957 WP_000593311.1 glutathione synthase -
  ACIFYW_RS11190 (ACIFYW_11190) - 2498876..2499439 (+) 564 WP_001054771.1 YqgE/AlgH family protein -
  ACIFYW_RS11195 (ACIFYW_11195) ruvX 2499520..2499942 (+) 423 WP_000091869.1 Holliday junction resolvase RuvX -
  ACIFYW_RS11200 (ACIFYW_11200) tyrS 2499967..2501247 (-) 1281 WP_179875178.1 tyrosine--tRNA ligase -
  ACIFYW_RS11205 (ACIFYW_11205) - 2501356..2502153 (-) 798 WP_179875179.1 helix-turn-helix transcriptional regulator -
  ACIFYW_RS11210 (ACIFYW_11210) pilU 2502271..2503377 (-) 1107 WP_000422572.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ACIFYW_RS11215 (ACIFYW_11215) pilT 2503392..2504429 (-) 1038 WP_000350195.1 type IV pilus twitching motility protein PilT Machinery gene
  ACIFYW_RS11220 (ACIFYW_11220) - 2504455..2505165 (+) 711 WP_404272602.1 YggS family pyridoxal phosphate-dependent enzyme -
  ACIFYW_RS11225 (ACIFYW_11225) proC 2505218..2506036 (+) 819 WP_258455240.1 pyrroline-5-carboxylate reductase -
  ACIFYW_RS11230 (ACIFYW_11230) - 2506090..2506647 (+) 558 WP_404272603.1 YggT family protein -
  ACIFYW_RS11235 (ACIFYW_11235) yggU 2506647..2506937 (+) 291 WP_000741914.1 DUF167 family protein YggU -
  ACIFYW_RS11240 (ACIFYW_11240) - 2506982..2507413 (+) 432 WP_069731232.1 DUF4426 domain-containing protein -
  ACIFYW_RS11245 (ACIFYW_11245) - 2507483..2508085 (+) 603 WP_057557453.1 XTP/dITP diphosphatase -

Sequence

Protein


Download         Length: 368 a.a.        Molecular weight: 41307.27 Da        Isoelectric Point: 6.5076

>NTDB_id=1070051 ACIFYW_RS11210 WP_000422572.1 2502271..2503377(-) (pilU) [Vibrio cholerae strain SPH 24-01]
MELNQYLDGMLTHKASDLYITVGAPILYRVDGELRAQGEALSVADVTALLHAMMDDARQAEFKQTREANFAVVRDSGRFR
VSAFFQRELPGAVIRRIETRIPTFEELKLPEVLQNLAIAKRGLVLVVGATGSGKSTTMAAMTGYRNQHRTGHILTVEDPI
EFVHEHKRCIVTQREVGLDTESYEVALKNSLRQAPDMILIGEIRSRETMEYAMTFAETGHLCMATLHANNANQALERILH
LVPKEQREQFLLDLSLNLKGVIAQQLLRDKNGKGRHGVFEVLLNSPRIADLIRRGELHELKATMARSQEVGMQTFDQALY
QLVVDDKISEQDALHSADSANDLRLMLKTKRGDDYGSGSLQNVKIDME

Nucleotide


Download         Length: 1107 bp        

>NTDB_id=1070051 ACIFYW_RS11210 WP_000422572.1 2502271..2503377(-) (pilU) [Vibrio cholerae strain SPH 24-01]
ATGGAGTTGAATCAATATCTGGATGGCATGCTGACTCATAAAGCATCGGATCTGTACATTACGGTGGGTGCACCGATTTT
GTATCGAGTCGATGGTGAATTGCGTGCACAAGGTGAAGCGCTCAGCGTGGCGGATGTGACGGCCTTATTGCATGCGATGA
TGGATGACGCAAGGCAAGCAGAATTTAAGCAGACGCGCGAGGCGAATTTTGCTGTGGTGCGTGACAGTGGCCGTTTTCGG
GTGAGTGCATTTTTCCAACGTGAATTGCCGGGGGCGGTGATCCGCCGGATTGAAACGCGCATTCCCACCTTTGAAGAACT
GAAATTGCCTGAAGTGCTGCAAAATTTGGCGATTGCCAAACGCGGTTTAGTGTTGGTGGTCGGCGCAACCGGTTCGGGTA
AATCGACCACTATGGCAGCCATGACTGGCTATCGTAATCAGCATCGCACCGGGCATATTTTGACGGTCGAAGATCCCATT
GAGTTTGTGCATGAACACAAGCGCTGTATCGTGACGCAGCGAGAAGTGGGGCTCGATACCGAAAGTTATGAAGTGGCGCT
GAAAAACTCGCTGCGCCAAGCCCCCGATATGATTTTGATTGGTGAAATCCGCAGCCGAGAAACCATGGAATACGCGATGA
CCTTCGCGGAAACCGGGCATTTGTGTATGGCCACGCTGCACGCCAATAACGCCAACCAAGCGTTAGAGCGCATTTTGCAT
TTAGTGCCGAAAGAGCAGCGTGAGCAGTTTTTGCTCGATTTGTCACTCAACCTCAAAGGCGTGATTGCCCAGCAATTATT
GCGCGATAAAAATGGCAAAGGTCGACACGGGGTGTTTGAAGTGTTGCTCAATAGCCCGCGTATCGCGGATTTAATTCGCC
GTGGTGAACTGCATGAGCTGAAAGCGACCATGGCTCGTTCGCAGGAAGTGGGGATGCAAACTTTCGATCAAGCCTTATAC
CAGCTTGTGGTGGATGACAAAATCAGTGAGCAAGATGCCCTGCACAGTGCCGATTCAGCCAACGATCTGCGTTTGATGCT
CAAAACCAAGCGTGGTGATGACTACGGCAGCGGCTCTTTGCAGAATGTGAAGATTGATATGGAGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

100

100

1
  pilU Pseudomonas stutzeri DSM 10701

58.857

95.109

0.56
  pilU Acinetobacter baylyi ADP1

54.701

95.38

0.522
  pilT Legionella pneumophila strain Lp02

42.773

92.12

0.394
  pilT Legionella pneumophila strain ERS1305867

42.773

92.12

0.394
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

45.806

84.239

0.386
  pilT Vibrio cholerae strain A1552

42.216

90.761

0.383
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

42.216

90.761

0.383
  pilT Acinetobacter baumannii D1279779

41.593

92.12

0.383
  pilT Acinetobacter baumannii strain A118

41.593

92.12

0.383
  pilT Acinetobacter nosocomialis M2

41.593

92.12

0.383
  pilT Pseudomonas aeruginosa PAK

41.298

92.12

0.38
  pilT Pseudomonas stutzeri DSM 10701

41.003

92.12

0.378
  pilT Acinetobacter baylyi ADP1

42.462

88.315

0.375
  pilT Neisseria gonorrhoeae MS11

38.329

94.293

0.361
  pilT Neisseria meningitidis 8013

38.551

93.75

0.361

Multiple sequence alignment