Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   RSP597_RS14315 Genome accession   NZ_CP172969
Coordinates   3000049..3001092 (+) Length   347 a.a.
NCBI ID   WP_003263111.1    Uniprot ID   -
Organism   Ralstonia solanacearum strain P597     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Genomic island 2968989..2999870 3000049..3001092 flank 179


Gene organization within MGE regions


Location: 2968989..3001092
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RSP597_RS14145 (RSP597_014145) - 2968989..2969531 (+) 543 WP_231107282.1 TniB family NTP-binding protein -
  RSP597_RS14150 (RSP597_014150) - 2969609..2969932 (+) 324 Protein_2768 TniQ family protein -
  RSP597_RS14155 (RSP597_014155) - 2970173..2970595 (+) 423 WP_231107284.1 hypothetical protein -
  RSP597_RS14160 (RSP597_014160) - 2970554..2972026 (-) 1473 WP_231107283.1 hypothetical protein -
  RSP597_RS14165 (RSP597_014165) - 2972335..2972688 (-) 354 WP_064045014.1 hypothetical protein -
  RSP597_RS14175 (RSP597_014175) - 2973955..2974842 (+) 888 WP_064047018.1 hypothetical protein -
  RSP597_RS14180 (RSP597_014180) - 2974856..2975779 (+) 924 WP_081262700.1 trypsin-like serine protease -
  RSP597_RS14185 (RSP597_014185) - 2975785..2976723 (+) 939 WP_155739655.1 hypothetical protein -
  RSP597_RS14190 (RSP597_014190) istB 2976802..2977560 (-) 759 WP_064046979.1 IS21-like element helper ATPase IstB -
  RSP597_RS14195 (RSP597_014195) istA 2977569..2979122 (-) 1554 WP_064046978.1 IS21 family transposase -
  RSP597_RS14200 (RSP597_014200) - 2979479..2980525 (+) 1047 WP_064046951.1 S8 family serine peptidase -
  RSP597_RS14205 (RSP597_014205) - 2980737..2981612 (-) 876 WP_155739645.1 hypothetical protein -
  RSP597_RS14210 (RSP597_014210) - 2981621..2984248 (-) 2628 WP_081262683.1 S8 family peptidase -
  RSP597_RS14215 (RSP597_014215) - 2984133..2985128 (-) 996 WP_081262684.1 AAA family ATPase -
  RSP597_RS14220 (RSP597_014220) - 2985211..2985732 (-) 522 WP_155739644.1 hypothetical protein -
  RSP597_RS14225 (RSP597_014225) tnpC 2985864..2987438 (-) 1575 WP_064046952.1 IS66 family transposase -
  RSP597_RS14230 (RSP597_014230) tnpB 2987470..2987820 (-) 351 WP_043946514.1 IS66 family insertion sequence element accessory protein TnpB -
  RSP597_RS14235 (RSP597_014235) - 2987796..2988320 (-) 525 WP_043946515.1 transposase -
  RSP597_RS14240 (RSP597_014240) - 2988366..2989166 (-) 801 WP_064046974.1 metallophosphoesterase -
  RSP597_RS14245 (RSP597_014245) - 2989593..2989922 (-) 330 WP_404943911.1 hypothetical protein -
  RSP597_RS14250 (RSP597_014250) - 2989951..2990310 (+) 360 WP_064046015.1 hypothetical protein -
  RSP597_RS14255 (RSP597_014255) tnpC 2990280..2991857 (-) 1578 WP_064046014.1 IS66 family transposase -
  RSP597_RS14260 (RSP597_014260) tnpB 2991887..2992234 (-) 348 WP_064046013.1 IS66 family insertion sequence element accessory protein TnpB -
  RSP597_RS14265 (RSP597_014265) tnpA 2992231..2992638 (-) 408 WP_081262565.1 IS66-like element accessory protein TnpA -
  RSP597_RS14270 (RSP597_014270) - 2992674..2993222 (-) 549 WP_306660207.1 AAA family ATPase -
  RSP597_RS14275 (RSP597_014275) - 2994173..2995117 (+) 945 WP_013205194.1 LysR substrate-binding domain-containing protein -
  RSP597_RS14280 (RSP597_014280) - 2995396..2995638 (+) 243 WP_003263116.1 hypothetical protein -
  RSP597_RS14285 (RSP597_014285) - 2995797..2996297 (+) 501 WP_003271865.1 Dps family protein -
  RSP597_RS14290 (RSP597_014290) ubiA 2996371..2997246 (+) 876 WP_064045017.1 4-hydroxybenzoate octaprenyltransferase -
  RSP597_RS14300 (RSP597_014300) - 2998058..2998336 (-) 279 WP_064045018.1 hypothetical protein -
  RSP597_RS14305 (RSP597_014305) proC 2998345..2999169 (-) 825 WP_013205197.1 pyrroline-5-carboxylate reductase -
  RSP597_RS14310 (RSP597_014310) - 2999205..2999927 (-) 723 WP_097908838.1 YggS family pyridoxal phosphate-dependent enzyme -
  RSP597_RS14315 (RSP597_014315) pilT 3000049..3001092 (+) 1044 WP_003263111.1 type IV pilus twitching motility protein PilT Machinery gene

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38166.80 Da        Isoelectric Point: 6.8705

>NTDB_id=1069776 RSP597_RS14315 WP_003263111.1 3000049..3001092(+) (pilT) [Ralstonia solanacearum strain P597]
MDIAQLLAFAAKNKASDLHLSAGLPPMIRIHGDMRRINVPPLTHQDVHAMVYDIMSDVQRKHYEENLEADFSFEIPGLSR
FRVNAFNQNRGAAAVFRTIPSKVLTLEDLKAPAVFSDLAMKPRGLVLVTGPTGSGKSTTLAAMVNHRNESDLGHILTVED
PIEFVHESKKSLINQRELGPHTHSFANALKSALREDPDVVLVGELRDLETIRLALTAAETGHLVFGTLHTSSAAKTIDRV
VDVFPSDEKDMVRTMLSESLEAVISQTLLKTRDGSGRVAAHEIMICTPAIRHLIRENKISQMYSMMQTSSGLGMQTLDQC
LAELIKRSAINYADARAIAKNPDAFAN

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=1069776 RSP597_RS14315 WP_003263111.1 3000049..3001092(+) (pilT) [Ralstonia solanacearum strain P597]
ATGGACATCGCGCAGCTTCTGGCCTTCGCCGCCAAGAACAAAGCGTCTGATCTGCACTTGTCGGCGGGCTTGCCACCGAT
GATCCGGATCCATGGCGACATGCGTCGCATCAACGTGCCGCCGCTCACGCACCAGGATGTCCACGCCATGGTGTACGACA
TCATGAGCGACGTGCAGCGCAAGCATTACGAAGAAAACCTGGAAGCGGACTTCTCGTTCGAGATTCCCGGGTTGTCGCGT
TTCCGGGTCAACGCCTTCAATCAGAACCGCGGCGCCGCCGCCGTGTTCCGGACGATTCCGTCCAAGGTCCTGACGCTGGA
AGACCTGAAGGCGCCCGCCGTCTTCTCCGACCTCGCCATGAAGCCGCGCGGCCTGGTGCTGGTGACGGGCCCGACCGGTT
CGGGCAAGTCGACCACGCTGGCGGCGATGGTCAACCACCGCAACGAAAGCGATCTGGGCCACATCCTCACGGTGGAGGAC
CCGATCGAATTCGTGCACGAATCCAAGAAGAGCCTGATCAACCAGCGTGAGCTGGGGCCGCATACGCACTCGTTCGCCAA
CGCGCTGAAATCGGCGCTGCGGGAAGACCCGGACGTGGTCCTGGTCGGCGAATTGCGGGACCTGGAAACCATCCGCCTGG
CACTGACGGCGGCCGAAACCGGCCACTTGGTGTTTGGCACGCTGCACACGAGTTCCGCGGCCAAGACCATCGACCGGGTG
GTCGACGTGTTCCCCTCGGATGAGAAGGACATGGTCCGCACCATGCTGTCCGAATCGCTGGAAGCGGTGATCTCGCAGAC
GCTGCTCAAGACGCGCGACGGCTCCGGCCGGGTCGCGGCGCACGAGATCATGATCTGCACGCCGGCCATCCGACACCTGA
TCCGCGAGAACAAGATTTCGCAGATGTATTCGATGATGCAGACCAGCAGCGGGCTGGGCATGCAGACGCTGGACCAATGC
CTGGCGGAGCTCATCAAGCGCTCGGCGATCAATTACGCCGACGCGCGCGCCATCGCCAAGAACCCGGACGCGTTCGCGAA
CTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

71.594

99.424

0.712
  pilT Acinetobacter baumannii D1279779

71.594

99.424

0.712
  pilT Acinetobacter baumannii strain A118

71.594

99.424

0.712
  pilT Pseudomonas aeruginosa PAK

71.304

99.424

0.709
  pilT Pseudomonas stutzeri DSM 10701

71.014

99.424

0.706
  pilT Acinetobacter baylyi ADP1

69.855

99.424

0.695
  pilT Neisseria gonorrhoeae MS11

68.497

99.712

0.683
  pilT Neisseria meningitidis 8013

68.497

99.712

0.683
  pilT Legionella pneumophila strain Lp02

67.826

99.424

0.674
  pilT Legionella pneumophila strain ERS1305867

67.826

99.424

0.674
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

66.469

97.118

0.646
  pilT Vibrio cholerae strain A1552

66.469

97.118

0.646
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.867

99.712

0.507
  pilU Acinetobacter baylyi ADP1

42.442

99.135

0.421
  pilU Pseudomonas stutzeri DSM 10701

42.388

96.542

0.409
  pilU Vibrio cholerae strain A1552

41.791

96.542

0.403

Multiple sequence alignment