Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   ACIHQR_RS08860 Genome accession   NZ_CP172866
Coordinates   2139688..2140800 (+) Length   370 a.a.
NCBI ID   WP_223740010.1    Uniprot ID   -
Organism   Corallococcus coralloides strain KYC2213     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2134688..2145800
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACIHQR_RS08840 (ACIHQR_08840) - 2134871..2135914 (-) 1044 WP_223740013.1 diguanylate cyclase -
  ACIHQR_RS08845 (ACIHQR_08845) - 2136090..2136710 (-) 621 WP_223740012.1 tRNA (guanosine(46)-N(7))-methyltransferase TrmB -
  ACIHQR_RS08850 (ACIHQR_08850) - 2136790..2137716 (+) 927 WP_404370049.1 bifunctional riboflavin kinase/FAD synthetase -
  ACIHQR_RS08855 (ACIHQR_08855) pilB 2137939..2139639 (+) 1701 WP_014399076.1 type IV-A pilus assembly ATPase PilB -
  ACIHQR_RS08860 (ACIHQR_08860) pilT 2139688..2140800 (+) 1113 WP_223740010.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ACIHQR_RS08865 (ACIHQR_08865) - 2140856..2142112 (+) 1257 WP_223740009.1 type II secretion system F family protein -
  ACIHQR_RS08870 (ACIHQR_08870) - 2142234..2143712 (+) 1479 WP_255651611.1 nitrogen regulation protein NR(II) -
  ACIHQR_RS08875 (ACIHQR_08875) - 2143709..2145142 (+) 1434 WP_223740008.1 sigma-54 dependent transcriptional regulator -

Sequence

Protein


Download         Length: 370 a.a.        Molecular weight: 40477.68 Da        Isoelectric Point: 8.2875

>NTDB_id=1069583 ACIHQR_RS08860 WP_223740010.1 2139688..2140800(+) (pilT) [Corallococcus coralloides strain KYC2213]
MANLHQLLKAMVEKGSSDLHITTGSPPQLRVDGELVPLKTAPLTPVETKQLCYSILTDAQKHKFEEENELDLSFGVKGLS
RFRANIFMQRGAVAGAFRTIPFKILTFQELGLPPVVAELVKKPRGLILVTGPTGSGKSTTLASMIDKINTERHEHIMTIE
DPIEYLHPHKNCLVNQREVGADTRNFKTALKYILRQDPDVVLVGELRDLETIEAALTIAETGHICYATLHTNSAVQTINR
ILDVFPPYQQPQVRAQLSFVLEGVMSQALVAKQGAPGRVLALEVMVPNPAIRNLIREDKVHQIYSSMQVGQAKYGMQTFN
QALAALLARRLISQDEAFGRSSDPEELRNILATGGGPGGAQRPAGGAGAR

Nucleotide


Download         Length: 1113 bp        

>NTDB_id=1069583 ACIHQR_RS08860 WP_223740010.1 2139688..2140800(+) (pilT) [Corallococcus coralloides strain KYC2213]
GTGGCCAACCTGCACCAGCTCCTCAAGGCGATGGTCGAGAAGGGCTCTTCCGACCTCCACATCACCACCGGCTCGCCGCC
GCAGCTCCGCGTGGACGGTGAGCTCGTCCCGCTCAAGACGGCGCCGCTCACGCCCGTGGAGACCAAGCAGCTCTGCTACT
CCATCCTCACGGACGCCCAGAAGCACAAGTTCGAGGAGGAGAACGAGCTGGACCTGTCGTTCGGCGTGAAGGGCCTGTCA
CGCTTCCGCGCGAACATCTTCATGCAGCGCGGCGCCGTCGCCGGTGCGTTCCGCACGATTCCCTTCAAGATCCTGACGTT
CCAGGAGCTGGGCCTGCCGCCGGTGGTGGCGGAGCTGGTGAAGAAGCCGCGCGGCCTCATCCTGGTGACGGGCCCCACGG
GCTCCGGCAAGTCCACCACGCTGGCCTCGATGATCGACAAGATCAACACCGAGCGTCATGAGCACATCATGACCATCGAG
GACCCCATCGAGTACCTGCACCCGCACAAGAACTGCCTGGTGAACCAGCGCGAGGTGGGTGCGGACACGCGCAACTTCAA
GACGGCCCTCAAGTACATCCTCCGCCAGGACCCGGACGTGGTGCTCGTCGGCGAGCTCCGCGACCTGGAGACCATCGAGG
CGGCGCTCACCATCGCGGAGACGGGCCACATCTGCTACGCGACGCTGCACACCAACAGCGCGGTGCAGACCATCAACCGC
ATCCTGGACGTCTTCCCGCCGTACCAGCAGCCCCAGGTGCGCGCCCAGTTGTCCTTCGTGCTGGAGGGCGTGATGAGCCA
GGCGCTCGTCGCCAAGCAGGGCGCTCCGGGCCGCGTGCTGGCCCTGGAGGTCATGGTGCCCAACCCCGCCATCCGGAACC
TCATCCGCGAGGACAAGGTCCACCAGATCTACTCGTCCATGCAGGTGGGGCAGGCGAAGTACGGCATGCAGACGTTCAAC
CAGGCGCTGGCCGCCCTCCTGGCGCGCCGGCTCATCTCCCAGGATGAGGCCTTCGGCCGCTCCTCCGACCCGGAGGAGCT
GCGCAACATCCTCGCGACGGGCGGTGGGCCGGGCGGAGCCCAGCGGCCGGCCGGGGGAGCGGGCGCCCGTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-260)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.275

98.378

0.495
  pilT Legionella pneumophila strain ERS1305867

54.03

90.541

0.489
  pilT Legionella pneumophila strain Lp02

54.03

90.541

0.489
  pilT Pseudomonas aeruginosa PAK

51.304

93.243

0.478
  pilT Pseudomonas stutzeri DSM 10701

51.304

93.243

0.478
  pilT Acinetobacter baylyi ADP1

51.014

93.243

0.476
  pilT Acinetobacter baumannii strain A118

50.725

93.243

0.473
  pilT Acinetobacter baumannii D1279779

50.725

93.243

0.473
  pilT Acinetobacter nosocomialis M2

50.435

93.243

0.47
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

48.802

90.27

0.441
  pilT Vibrio cholerae strain A1552

48.802

90.27

0.441
  pilT Neisseria gonorrhoeae MS11

44.868

92.162

0.414
  pilT Neisseria meningitidis 8013

44.868

92.162

0.414
  pilU Vibrio cholerae strain A1552

43.059

95.405

0.411
  pilU Pseudomonas stutzeri DSM 10701

41.011

96.216

0.395
  pilU Acinetobacter baylyi ADP1

40.346

93.784

0.378

Multiple sequence alignment