Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   AADG46_RS00915 Genome accession   NZ_AP028804
Coordinates   174469..175575 (+) Length   368 a.a.
NCBI ID   WP_000422573.1    Uniprot ID   -
Organism   Vibrio cholerae strain VN152     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 169469..180575
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AADG46_RS00880 (VNVC001_01330) - 169761..170363 (-) 603 WP_000725016.1 XTP/dITP diphosphatase -
  AADG46_RS00885 (VNVC001_01340) - 170433..170864 (-) 432 WP_002046367.1 DUF4426 domain-containing protein -
  AADG46_RS00890 (VNVC001_01350) yggU 170909..171199 (-) 291 WP_000741914.1 DUF167 family protein YggU -
  AADG46_RS00895 (VNVC001_01360) - 171199..171756 (-) 558 WP_001087261.1 YggT family protein -
  AADG46_RS00900 (VNVC001_01370) proC 171810..172628 (-) 819 WP_113628462.1 pyrroline-5-carboxylate reductase -
  AADG46_RS00905 (VNVC001_01380) - 172681..173391 (-) 711 WP_340701875.1 YggS family pyridoxal phosphate-dependent enzyme -
  AADG46_RS00910 (VNVC001_01390) pilT 173417..174454 (+) 1038 WP_000350195.1 type IV pilus twitching motility protein PilT Machinery gene
  AADG46_RS00915 (VNVC001_01400) pilU 174469..175575 (+) 1107 WP_000422573.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  AADG46_RS00920 (VNVC001_01410) - 175693..176490 (+) 798 WP_340701876.1 helix-turn-helix transcriptional regulator -
  AADG46_RS00925 (VNVC001_01420) tyrS 176599..177879 (+) 1281 WP_340701877.1 tyrosine--tRNA ligase -
  AADG46_RS00930 (VNVC001_01430) ruvX 177904..178326 (-) 423 WP_029627830.1 Holliday junction resolvase RuvX -
  AADG46_RS00935 (VNVC001_01440) - 178407..178970 (-) 564 WP_001054771.1 YqgE/AlgH family protein -
  AADG46_RS00940 (VNVC001_01450) gshB 179006..179962 (-) 957 WP_000593313.1 glutathione synthase -

Sequence

Protein


Download         Length: 368 a.a.        Molecular weight: 41306.29 Da        Isoelectric Point: 6.6415

>NTDB_id=106896 AADG46_RS00915 WP_000422573.1 174469..175575(+) (pilU) [Vibrio cholerae strain VN152]
MELNQYLDGMLTHKASDLYITVGAPILYRVDGELRAQGEALSVADVTALLHAMMDDARQAEFKQTREANFAVVRDSGRFR
VSAFFQRELPGAVIRRIETRIPTFEELKLPEVLQNLAIAKRGLVLVVGATGSGKSTTMAAMTGYRNQHRTGHILTVEDPI
EFVHEHKRCIVTQREVGLDTESYEVALKNSLRQAPDMILIGEIRSRETMEYAMTFAETGHLCMATLHANNANQALERILH
LVPKEQREQFLLDLSLNLKGVIAQQLLRDKNGKGRHGVFEVLLNSPRIADLIRRGELHELKATMARSQEVGMQTFDQALY
QLVVDDKISEQDALHSADSANDLRLMLKTKRGNDYGSGSLQNVKIDME

Nucleotide


Download         Length: 1107 bp        

>NTDB_id=106896 AADG46_RS00915 WP_000422573.1 174469..175575(+) (pilU) [Vibrio cholerae strain VN152]
ATGGAGTTGAATCAATATCTGGATGGCATGCTGACTCATAAAGCATCGGATCTGTACATTACGGTGGGTGCACCGATTTT
GTATCGAGTCGATGGTGAATTGCGTGCGCAAGGTGAAGCGCTCAGCGTGGCGGATGTGACGGCCTTATTGCATGCGATGA
TGGATGACGCAAGGCAAGCAGAATTTAAGCAGACGCGCGAAGCGAACTTTGCTGTGGTGCGTGACAGTGGCCGTTTTCGG
GTGAGTGCATTTTTCCAACGTGAATTGCCGGGGGCGGTGATCCGCCGGATTGAAACGCGCATTCCGACCTTTGAAGAACT
GAAGTTGCCTGAAGTGCTGCAAAATTTGGCGATTGCCAAACGCGGTTTAGTGTTGGTGGTCGGCGCAACCGGTTCGGGTA
AATCGACCACTATGGCAGCCATGACTGGCTATCGTAATCAGCATCGCACCGGGCATATTTTGACGGTCGAAGATCCAATT
GAGTTTGTGCATGAACACAAGCGCTGTATCGTGACGCAGCGAGAAGTGGGGTTGGATACCGAAAGTTATGAAGTGGCGCT
GAAAAACTCGCTGCGCCAAGCCCCCGATATGATTTTGATTGGCGAAATTCGCAGCCGAGAAACCATGGAATACGCGATGA
CTTTCGCTGAAACCGGGCATTTGTGTATGGCCACGCTGCACGCTAACAATGCCAACCAAGCCTTAGAGCGGATTTTGCAT
CTGGTGCCCAAAGAGCAGCGTGAGCAGTTTTTGCTCGATTTGTCACTCAACCTCAAAGGCGTGATTGCCCAGCAATTATT
GCGCGATAAAAATGGCAAAGGTCGACACGGGGTGTTTGAAGTGTTGCTCAATAGCCCGCGTATCGCGGATTTAATTCGCC
GCGGTGAACTGCATGAGCTGAAAGCGACCATGGCTCGTTCGCAGGAAGTGGGGATGCAAACTTTCGATCAAGCCTTATAC
CAGCTTGTGGTGGATGACAAAATCAGTGAGCAAGATGCCCTGCACAGTGCCGATTCAGCCAACGATCTGCGTTTGATGCT
CAAAACCAAGCGTGGTAATGACTACGGCAGCGGCTCTTTGCAGAATGTGAAGATTGATATGGAGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

99.728

100

0.997
  pilU Pseudomonas stutzeri DSM 10701

58.857

95.109

0.56
  pilU Acinetobacter baylyi ADP1

54.701

95.38

0.522
  pilT Legionella pneumophila strain Lp02

42.773

92.12

0.394
  pilT Legionella pneumophila strain ERS1305867

42.773

92.12

0.394
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

45.806

84.239

0.386
  pilT Vibrio cholerae strain A1552

42.216

90.761

0.383
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

42.216

90.761

0.383
  pilT Acinetobacter baumannii D1279779

41.593

92.12

0.383
  pilT Acinetobacter baumannii strain A118

41.593

92.12

0.383
  pilT Acinetobacter nosocomialis M2

41.593

92.12

0.383
  pilT Pseudomonas aeruginosa PAK

41.298

92.12

0.38
  pilT Pseudomonas stutzeri DSM 10701

41.003

92.12

0.378
  pilT Acinetobacter baylyi ADP1

42.462

88.315

0.375
  pilT Neisseria gonorrhoeae MS11

38.329

94.293

0.361
  pilT Neisseria meningitidis 8013

38.551

93.75

0.361

Multiple sequence alignment