Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   ACHZDB_RS08105 Genome accession   NZ_CP172405
Coordinates   1811446..1812486 (-) Length   346 a.a.
NCBI ID   WP_005425810.1    Uniprot ID   A0A9X3MNX0
Organism   Vibrio campbellii strain LB503     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1806446..1817486
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACHZDB_RS08075 (ACHZDB_08075) endA 1806542..1807237 (+) 696 WP_399423718.1 deoxyribonuclease I -
  ACHZDB_RS08080 (ACHZDB_08080) rsmE 1807384..1808115 (+) 732 WP_039985539.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  ACHZDB_RS08085 (ACHZDB_08085) gshB 1808130..1809080 (+) 951 WP_005425823.1 glutathione synthase -
  ACHZDB_RS08090 (ACHZDB_08090) - 1809210..1809773 (+) 564 WP_010450344.1 YqgE/AlgH family protein -
  ACHZDB_RS08095 (ACHZDB_08095) ruvX 1809821..1810246 (+) 426 WP_399423721.1 Holliday junction resolvase RuvX -
  ACHZDB_RS08100 (ACHZDB_08100) pilU 1810308..1811414 (-) 1107 WP_005425812.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ACHZDB_RS08105 (ACHZDB_08105) pilT 1811446..1812486 (-) 1041 WP_005425810.1 type IV pilus twitching motility protein PilT Machinery gene
  ACHZDB_RS08110 (ACHZDB_08110) - 1812515..1813225 (+) 711 WP_005425809.1 YggS family pyridoxal phosphate-dependent enzyme -
  ACHZDB_RS08115 (ACHZDB_08115) proC 1813356..1814174 (+) 819 WP_399423723.1 pyrroline-5-carboxylate reductase -
  ACHZDB_RS08120 (ACHZDB_08120) - 1814228..1814785 (+) 558 WP_038892074.1 YggT family protein -
  ACHZDB_RS08125 (ACHZDB_08125) yggU 1814785..1815075 (+) 291 WP_005425805.1 DUF167 family protein YggU -
  ACHZDB_RS08130 (ACHZDB_08130) - 1815135..1815566 (+) 432 WP_399423725.1 DUF4426 domain-containing protein -
  ACHZDB_RS08135 (ACHZDB_08135) - 1815672..1816274 (+) 603 WP_399423727.1 XTP/dITP diphosphatase -
  ACHZDB_RS08140 (ACHZDB_08140) hemW 1816293..1817465 (+) 1173 WP_103412674.1 radical SAM family heme chaperone HemW -

Sequence

Protein


Download         Length: 346 a.a.        Molecular weight: 38634.12 Da        Isoelectric Point: 6.5043

>NTDB_id=1067441 ACHZDB_RS08105 WP_005425810.1 1811446..1812486(-) (pilT) [Vibrio campbellii strain LB503]
MDITELLDFSVKHNASDLHLSAGVPPMVRIDGDVRKLGIPAFTHQEVHRLVFEIMNDAQRSEFEEKLEVDFSFELHNVGR
FRVNAFNQSRGCAAVFRTIPSSIPTLEELEAPEIFRKIANAEKGLVLVTGPTGSGKSTTLAAIVDYINRNHNKHVLTIED
PIEFVHTNNKCLINQREVHRDTHSFQNALRSALREDPDVILVGEMRDKETISLALTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPGSDKDMVRSMLSESLRSVIAQKLLKRNGGGRIACHEIMMATPAIRNLIREDKVAQMYSIIQTGAAHGMQTMEQNA
RQLMAQGMVSREEVDKKVEIEAQQFS

Nucleotide


Download         Length: 1041 bp        

>NTDB_id=1067441 ACHZDB_RS08105 WP_005425810.1 1811446..1812486(-) (pilT) [Vibrio campbellii strain LB503]
ATGGATATCACTGAGTTACTGGATTTTAGTGTAAAACATAATGCTTCGGATCTACATCTTTCTGCAGGTGTACCACCAAT
GGTTCGTATTGATGGTGATGTAAGAAAACTGGGCATTCCAGCGTTTACACACCAAGAAGTGCACCGTTTGGTTTTTGAAA
TCATGAACGATGCGCAGCGCAGTGAGTTCGAAGAAAAACTCGAAGTCGACTTTTCATTTGAATTGCATAACGTTGGCCGT
TTTCGTGTCAACGCTTTCAACCAATCTCGCGGTTGTGCGGCGGTGTTCCGTACCATCCCAAGTAGCATTCCAACACTGGA
AGAGTTAGAAGCTCCTGAGATCTTCAGAAAAATCGCCAATGCGGAAAAAGGTTTGGTACTGGTTACTGGCCCAACGGGTT
CTGGTAAATCGACTACCTTGGCTGCCATTGTTGACTACATTAACCGTAATCACAATAAGCACGTCCTAACAATTGAAGAT
CCGATTGAATTTGTTCACACCAACAATAAGTGTCTGATCAACCAACGTGAAGTACACCGTGATACACACAGCTTCCAAAA
CGCGCTGCGCAGCGCGCTGCGTGAAGATCCGGATGTGATTCTAGTTGGTGAGATGCGTGATAAAGAAACCATCAGTTTGG
CGCTGACGGCTGCTGAAACGGGTCACCTTGTTTTCGGTACGCTGCACACTAGCTCGGCAGCGAAAACCATCGACCGTATT
ATTGACGTCTTCCCTGGTAGCGACAAAGACATGGTGCGTTCGATGCTGTCAGAATCATTGCGTTCGGTTATCGCGCAAAA
ACTTCTGAAACGTAACGGCGGCGGTCGTATCGCGTGTCATGAAATAATGATGGCAACGCCTGCGATCCGTAACCTTATTC
GTGAAGATAAAGTCGCGCAGATGTATTCGATCATTCAGACTGGTGCGGCGCATGGTATGCAGACCATGGAGCAGAATGCT
CGTCAATTGATGGCGCAAGGCATGGTATCGCGTGAAGAAGTGGATAAGAAAGTTGAAATTGAAGCGCAGCAATTCTCGTA
A

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

86.377

99.711

0.861
  pilT Vibrio cholerae strain A1552

86.377

99.711

0.861
  pilT Acinetobacter baumannii D1279779

72.121

95.376

0.688
  pilT Acinetobacter baumannii strain A118

72.121

95.376

0.688
  pilT Acinetobacter nosocomialis M2

71.818

95.376

0.685
  pilT Acinetobacter baylyi ADP1

71.515

95.376

0.682
  pilT Pseudomonas aeruginosa PAK

68.824

98.266

0.676
  pilT Pseudomonas stutzeri DSM 10701

69.027

97.977

0.676
  pilT Legionella pneumophila strain Lp02

67.674

95.665

0.647
  pilT Legionella pneumophila strain ERS1305867

67.674

95.665

0.647
  pilT Neisseria meningitidis 8013

64.118

98.266

0.63
  pilT Neisseria gonorrhoeae MS11

63.824

98.266

0.627
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.857

100

0.503
  pilU Vibrio cholerae strain A1552

41.617

96.532

0.402
  pilU Pseudomonas stutzeri DSM 10701

40.419

96.532

0.39

Multiple sequence alignment