Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   ACHZDB_RS08100 Genome accession   NZ_CP172405
Coordinates   1810308..1811414 (-) Length   368 a.a.
NCBI ID   WP_005425812.1    Uniprot ID   A7MTP8
Organism   Vibrio campbellii strain LB503     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1805308..1816414
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACHZDB_RS08070 (ACHZDB_08070) - 1805894..1806391 (+) 498 WP_012128940.1 SprT family zinc-dependent metalloprotease -
  ACHZDB_RS08075 (ACHZDB_08075) endA 1806542..1807237 (+) 696 WP_399423718.1 deoxyribonuclease I -
  ACHZDB_RS08080 (ACHZDB_08080) rsmE 1807384..1808115 (+) 732 WP_039985539.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  ACHZDB_RS08085 (ACHZDB_08085) gshB 1808130..1809080 (+) 951 WP_005425823.1 glutathione synthase -
  ACHZDB_RS08090 (ACHZDB_08090) - 1809210..1809773 (+) 564 WP_010450344.1 YqgE/AlgH family protein -
  ACHZDB_RS08095 (ACHZDB_08095) ruvX 1809821..1810246 (+) 426 WP_399423721.1 Holliday junction resolvase RuvX -
  ACHZDB_RS08100 (ACHZDB_08100) pilU 1810308..1811414 (-) 1107 WP_005425812.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ACHZDB_RS08105 (ACHZDB_08105) pilT 1811446..1812486 (-) 1041 WP_005425810.1 type IV pilus twitching motility protein PilT Machinery gene
  ACHZDB_RS08110 (ACHZDB_08110) - 1812515..1813225 (+) 711 WP_005425809.1 YggS family pyridoxal phosphate-dependent enzyme -
  ACHZDB_RS08115 (ACHZDB_08115) proC 1813356..1814174 (+) 819 WP_399423723.1 pyrroline-5-carboxylate reductase -
  ACHZDB_RS08120 (ACHZDB_08120) - 1814228..1814785 (+) 558 WP_038892074.1 YggT family protein -
  ACHZDB_RS08125 (ACHZDB_08125) yggU 1814785..1815075 (+) 291 WP_005425805.1 DUF167 family protein YggU -
  ACHZDB_RS08130 (ACHZDB_08130) - 1815135..1815566 (+) 432 WP_399423725.1 DUF4426 domain-containing protein -
  ACHZDB_RS08135 (ACHZDB_08135) - 1815672..1816274 (+) 603 WP_399423727.1 XTP/dITP diphosphatase -

Sequence

Protein


Download         Length: 368 a.a.        Molecular weight: 41335.48 Da        Isoelectric Point: 6.7731

>NTDB_id=1067440 ACHZDB_RS08100 WP_005425812.1 1810308..1811414(-) (pilU) [Vibrio campbellii strain LB503]
MDLNKFLEGMLALKASDLYITVGAPILFRVDGELRPQGDKLTENDVALLLDSAMEPERRQEFRKSRESNFAIVRDCGRFR
VSAFFQRELPGAVIRRIETNIPTFEQLKLPLVLQDLAIAKRGLVLVVGATGSGKSTTMAAMTGYRNNNKTGHILTVEDPI
EFVHEHKRCIVTQREVGLDTDSYEVALKNSLRQAPDMILIGEIRSRETMEYAMTFAETGHLCMATLHANNANQALERILH
LVPKDQKDQFLFDLSMNLKGVVGQQLIRDKNGQGRHGVFEILLNSPRVSDLIRRGDLHELKSTMARSNEFGMLTFDQSLY
KLVMQGKISEEDALHSADSANDLRLMLKTQRGEAFSTGTLANVKIDMD

Nucleotide


Download         Length: 1107 bp        

>NTDB_id=1067440 ACHZDB_RS08100 WP_005425812.1 1810308..1811414(-) (pilU) [Vibrio campbellii strain LB503]
ATGGATCTGAATAAATTTCTCGAAGGCATGTTGGCGTTAAAAGCGTCGGATCTCTACATCACTGTCGGTGCTCCGATTTT
GTTTCGTGTGGATGGTGAATTGCGCCCGCAAGGCGATAAGCTGACTGAAAATGATGTCGCCTTGTTACTTGACAGTGCAA
TGGAACCAGAGCGACGCCAGGAGTTTCGTAAAAGCCGTGAGTCTAACTTTGCCATTGTCCGTGATTGCGGTCGTTTCCGT
GTCAGTGCCTTTTTTCAACGTGAATTACCGGGAGCCGTTATTCGTCGTATTGAAACCAATATTCCGACGTTTGAGCAACT
TAAGTTACCTTTGGTACTGCAAGATCTTGCTATTGCCAAGCGTGGTTTAGTGCTGGTGGTTGGTGCCACTGGTTCTGGTA
AGTCGACCACTATGGCGGCGATGACGGGTTATCGCAACAACAACAAAACGGGTCATATCCTGACGGTAGAAGACCCGATT
GAATTTGTGCACGAACATAAGCGTTGCATCGTGACACAGCGTGAAGTTGGCTTAGACACCGACAGCTATGAAGTAGCGTT
GAAAAACTCGTTACGTCAGGCGCCAGATATGATTTTGATTGGTGAGATCCGTAGCCGAGAAACGATGGAATACGCGATGA
CCTTTGCTGAGACTGGTCACTTGTGTATGGCGACACTGCACGCCAACAACGCAAACCAAGCGCTGGAGCGTATTTTGCAC
CTAGTGCCGAAAGATCAGAAAGATCAGTTCTTATTTGATCTGTCGATGAACTTAAAAGGTGTGGTGGGTCAGCAGTTGAT
CCGTGATAAGAACGGTCAGGGGCGTCATGGCGTGTTTGAGATCTTATTAAACAGTCCGCGTGTTTCTGATCTGATCCGTC
GAGGTGATCTGCATGAATTGAAATCGACCATGGCTCGCTCTAACGAGTTTGGGATGCTGACCTTCGACCAATCGCTCTAC
AAGTTGGTGATGCAAGGTAAGATTAGCGAAGAGGATGCATTGCACAGTGCAGATTCAGCCAACGATCTGCGTTTGATGTT
GAAGACGCAACGTGGTGAAGCCTTCTCTACAGGCACGCTGGCGAATGTTAAGATCGATATGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(14-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A7MTP8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

81.25

100

0.813
  pilU Pseudomonas stutzeri DSM 10701

58.757

96.196

0.565
  pilU Acinetobacter baylyi ADP1

55.556

95.38

0.53
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.308

88.315

0.391
  pilT Acinetobacter baylyi ADP1

41.86

93.478

0.391
  pilT Acinetobacter nosocomialis M2

42.183

92.12

0.389
  pilT Acinetobacter baumannii D1279779

42.183

92.12

0.389
  pilT Acinetobacter baumannii strain A118

42.183

92.12

0.389
  pilT Legionella pneumophila strain Lp02

42.183

92.12

0.389
  pilT Legionella pneumophila strain ERS1305867

42.183

92.12

0.389
  pilT Pseudomonas aeruginosa PAK

41.888

92.12

0.386
  pilT Pseudomonas stutzeri DSM 10701

40.936

92.935

0.38
  pilT Vibrio cholerae strain A1552

41.317

90.761

0.375
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

41.317

90.761

0.375

Multiple sequence alignment