Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   ACHZ97_RS03195 Genome accession   NZ_CP172310
Coordinates   699231..700343 (-) Length   370 a.a.
NCBI ID   WP_115841327.1    Uniprot ID   A0A3D8VHC5
Organism   Lysobacter soli strain P18     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 694231..705343
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACHZ97_RS03165 (ACHZ97_03165) - 694805..695953 (-) 1149 WP_396616640.1 (Fe-S)-binding protein -
  ACHZ97_RS03170 (ACHZ97_03170) rplM 696181..696609 (+) 429 WP_115841323.1 50S ribosomal protein L13 -
  ACHZ97_RS03175 (ACHZ97_03175) rpsI 696636..697028 (+) 393 WP_115841324.1 30S ribosomal protein S9 -
  ACHZ97_RS03185 (ACHZ97_03185) - 697344..698333 (+) 990 WP_396616641.1 fumarylacetoacetate hydrolase family protein -
  ACHZ97_RS03190 (ACHZ97_03190) maiA 698397..699062 (+) 666 WP_115841326.1 maleylacetoacetate isomerase -
  ACHZ97_RS03195 (ACHZ97_03195) pilU 699231..700343 (-) 1113 WP_115841327.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ACHZ97_RS03200 (ACHZ97_03200) - 700336..700830 (+) 495 WP_386840875.1 DUF4398 domain-containing protein -
  ACHZ97_RS03205 (ACHZ97_03205) - 700827..701711 (+) 885 WP_396616642.1 OmpA family protein -
  ACHZ97_RS03210 (ACHZ97_03210) - 702101..702319 (+) 219 WP_115841330.1 YdcH family protein -
  ACHZ97_RS03215 (ACHZ97_03215) - 702553..703929 (+) 1377 WP_396616643.1 pyridoxal-phosphate dependent enzyme -
  ACHZ97_RS03220 (ACHZ97_03220) - 703929..704873 (+) 945 WP_396616644.1 2OG-Fe(II) oxygenase family protein -

Sequence

Protein


Download         Length: 370 a.a.        Molecular weight: 41126.43 Da        Isoelectric Point: 5.8003

>NTDB_id=1066211 ACHZ97_RS03195 WP_115841327.1 699231..700343(-) (pilU) [Lysobacter soli strain P18]
MDIGYFLKLMTEKNASDMFLTTGAPVYIKVEGKLYPLGNTGLPPGMVKKIAYSLMDEGQVPLFERDLELNMALALPEAGR
FRINVFKQRGEVGMVIRAIRSVIPSIEELQLPQVLKDIIMAPRGLVLIVGSTGSGKSTTLASMIDYRNSNISGHILTIED
PIEYLHKHKKSIVNQREVGLDTHAFHNALKNAMREAPDVILIGEILDATTMEAAIAFAETGHLCLATLHSNNADQTLERI
LNFFPEAAHKNVLMNLALNLRAVVSQRLVVGTDGRRVPAAEVLINTPHIRDLMRRGQVHEIKQAMEESLEEGMESFDQCL
FRLYKEGRIDMEEALKAADSREGLSLKFRLSEGSSGEHDPYAEIYDTATQ

Nucleotide


Download         Length: 1113 bp        

>NTDB_id=1066211 ACHZ97_RS03195 WP_115841327.1 699231..700343(-) (pilU) [Lysobacter soli strain P18]
ATGGACATCGGCTATTTCCTGAAGCTGATGACGGAAAAGAACGCGTCGGACATGTTCCTGACGACCGGCGCACCGGTTTA
TATCAAGGTCGAAGGCAAGCTCTACCCCCTCGGCAATACCGGCCTGCCCCCCGGCATGGTCAAGAAGATCGCCTATTCGT
TGATGGACGAGGGCCAGGTTCCGCTTTTCGAGCGTGACCTCGAGCTGAACATGGCGCTCGCCCTGCCCGAAGCCGGCCGC
TTCCGCATCAACGTCTTCAAGCAGCGCGGCGAAGTCGGCATGGTGATCCGCGCCATCCGCAGCGTGATTCCGTCGATCGA
GGAACTGCAGCTGCCGCAGGTGCTGAAGGACATCATCATGGCCCCGCGCGGCCTGGTGCTGATCGTCGGCTCCACCGGTT
CGGGCAAGTCGACCACGCTCGCGTCGATGATCGACTACCGCAACAGCAACATCTCGGGCCACATCCTCACCATCGAGGAT
CCGATCGAGTACCTGCACAAGCACAAGAAGTCGATCGTGAACCAGCGCGAGGTCGGCCTGGATACGCACGCCTTCCACAA
CGCGCTGAAGAACGCGATGCGCGAGGCGCCGGACGTCATCCTCATCGGCGAAATCCTCGATGCCACGACGATGGAGGCGG
CGATCGCCTTCGCCGAAACCGGCCACCTGTGCCTGGCGACGCTGCACTCCAACAACGCCGACCAGACGCTGGAGCGCATC
CTCAACTTCTTCCCGGAAGCGGCGCACAAGAACGTGCTGATGAACCTCGCGCTCAACCTGCGCGCGGTGGTCAGCCAGCG
CCTGGTCGTCGGCACCGACGGCCGCCGCGTGCCGGCGGCGGAAGTGCTGATCAACACGCCGCACATCCGCGACCTCATGC
GCCGCGGCCAGGTGCACGAGATCAAGCAGGCGATGGAGGAGTCGCTGGAGGAAGGCATGGAGTCTTTCGACCAGTGCCTG
TTCCGCCTCTACAAGGAAGGCCGCATCGACATGGAAGAGGCGCTCAAGGCCGCCGACTCCCGCGAAGGCCTCTCGCTCAA
GTTCCGCCTGTCGGAAGGCTCGAGCGGCGAACACGATCCGTACGCGGAAATCTACGACACGGCGACGCAGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(61-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A3D8VHC5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

52.089

97.027

0.505
  pilU Acinetobacter baylyi ADP1

48.87

95.676

0.468
  pilU Vibrio cholerae strain A1552

48.023

95.676

0.459
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

45.251

96.757

0.438
  pilT Acinetobacter baumannii strain A118

41.57

92.973

0.386
  pilT Acinetobacter nosocomialis M2

41.57

92.973

0.386
  pilT Acinetobacter baumannii D1279779

41.57

92.973

0.386
  pilT Pseudomonas aeruginosa PAK

42.012

91.351

0.384
  pilT Acinetobacter baylyi ADP1

41.279

92.973

0.384
  pilT Pseudomonas stutzeri DSM 10701

40.698

92.973

0.378
  pilT Legionella pneumophila strain Lp02

40.828

91.351

0.373
  pilT Legionella pneumophila strain ERS1305867

40.828

91.351

0.373
  pilT Neisseria gonorrhoeae MS11

39.769

93.784

0.373
  pilT Neisseria meningitidis 8013

39.769

93.784

0.373
  pilT Vibrio cholerae strain A1552

40.541

90

0.365
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

40.541

90

0.365

Multiple sequence alignment