Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   ACHMW6_RS04135 Genome accession   NZ_CP171977
Coordinates   756587..757630 (-) Length   347 a.a.
NCBI ID   WP_395405796.1    Uniprot ID   -
Organism   Pseudoduganella sp. UC29_106     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 751587..762630
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACHMW6_RS04125 (ACHMW6_04115) - 754961..755446 (-) 486 WP_395405794.1 glutathione peroxidase -
  ACHMW6_RS04130 (ACHMW6_04120) - 755446..756581 (-) 1136 Protein_722 PilT/PilU family type 4a pilus ATPase -
  ACHMW6_RS04135 (ACHMW6_04125) pilT 756587..757630 (-) 1044 WP_395405796.1 type IV pilus twitching motility protein PilT Machinery gene
  ACHMW6_RS04140 (ACHMW6_04130) - 757667..758375 (+) 709 Protein_724 YggS family pyridoxal phosphate-dependent enzyme -
  ACHMW6_RS04145 (ACHMW6_04135) proC 758382..759218 (+) 837 WP_395405798.1 pyrroline-5-carboxylate reductase -
  ACHMW6_RS04150 (ACHMW6_04140) - 759215..759598 (-) 384 WP_395405800.1 YqjD family protein -
  ACHMW6_RS04155 (ACHMW6_04145) ubiA 759629..760504 (-) 876 WP_395405802.1 4-hydroxybenzoate octaprenyltransferase -
  ACHMW6_RS04160 (ACHMW6_04150) - 760539..761477 (-) 939 WP_395405804.1 hydrogen peroxide-inducible genes activator -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38060.75 Da        Isoelectric Point: 6.7269

>NTDB_id=1064690 ACHMW6_RS04135 WP_395405796.1 756587..757630(-) (pilT) [Pseudoduganella sp. UC29_106]
MDISELLAFSVKNKASDLHLSAGLPPMIRVHGDVRRINLPPLEHKDVHGMIYDIMNDGQRKAYEEMLECDFSFAIPGLAR
FRVNAYNQERGASAVLRTIPSKILSLEELNAPKIFAEFALRPRGLVLVTGPTGSGKSTTLAAMINHLNENEYGHILTIED
PIEFVHESKKCLINQREVGPHTLSFNNALRSALREDPDAILVGELRDLETIRLALSAAETGHLVFGTLHTSSAAKTIDRI
IDVFPAEEKEMVRAMLSESLQAVISQTLLKTKDGSGRVAAHEIMVGTPAIRNLIREAKIAQMYSAIQTGSNVGMQTLDQN
LTDLLRRNVISAAAARSAAKIPENFPG

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=1064690 ACHMW6_RS04135 WP_395405796.1 756587..757630(-) (pilT) [Pseudoduganella sp. UC29_106]
ATGGACATTTCCGAACTTCTCGCATTCTCCGTCAAGAACAAAGCTTCCGACTTGCACCTCTCCGCCGGCCTGCCGCCGAT
GATCCGCGTTCACGGCGATGTCCGCCGCATCAATCTGCCCCCGCTCGAACACAAGGACGTGCACGGCATGATTTATGACA
TCATGAACGACGGCCAGCGCAAGGCGTATGAAGAGATGCTGGAGTGCGACTTTTCGTTTGCCATTCCCGGCCTGGCGCGC
TTCCGCGTCAACGCCTACAACCAGGAGCGCGGCGCATCGGCCGTGCTGCGTACCATCCCGTCCAAGATCCTGTCGCTGGA
AGAACTGAATGCGCCGAAGATCTTCGCCGAATTCGCGCTCAGGCCGCGCGGCCTGGTGCTGGTGACCGGCCCCACCGGCT
CCGGCAAATCGACCACGCTGGCCGCAATGATTAACCATTTAAACGAAAACGAGTACGGCCACATCCTGACCATCGAGGAC
CCGATCGAATTCGTGCACGAATCGAAGAAGTGCCTGATCAACCAGCGCGAAGTGGGCCCGCACACGCTGTCCTTCAACAA
TGCGCTGCGCTCCGCGCTGCGGGAAGACCCGGACGCGATCCTGGTCGGCGAATTGCGCGACCTGGAGACCATCCGCCTGG
CGTTGTCCGCCGCAGAAACGGGCCACCTGGTGTTCGGCACCCTGCACACGTCCTCCGCCGCCAAGACCATCGACCGTATC
ATCGACGTGTTCCCGGCCGAGGAAAAAGAGATGGTGCGCGCCATGCTGTCCGAATCGCTGCAGGCCGTGATCTCGCAGAC
GCTGCTGAAAACCAAGGACGGTTCCGGCCGCGTAGCCGCCCACGAAATCATGGTCGGCACGCCCGCCATCCGTAACCTGA
TCCGCGAAGCGAAAATCGCGCAGATGTATTCGGCGATCCAGACCGGCAGCAATGTCGGCATGCAGACGCTGGACCAGAAC
CTGACCGATCTCTTGCGCCGCAATGTCATCTCCGCCGCAGCTGCGCGTTCGGCTGCCAAGATCCCCGAAAACTTCCCAGG
CTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

77.101

99.424

0.767
  pilT Acinetobacter baumannii D1279779

77.101

99.424

0.767
  pilT Acinetobacter baumannii strain A118

77.101

99.424

0.767
  pilT Pseudomonas aeruginosa PAK

76.522

99.424

0.761
  pilT Pseudomonas stutzeri DSM 10701

75.072

99.424

0.746
  pilT Acinetobacter baylyi ADP1

75.072

99.424

0.746
  pilT Legionella pneumophila strain Lp02

75.516

97.695

0.738
  pilT Legionella pneumophila strain ERS1305867

75.516

97.695

0.738
  pilT Neisseria gonorrhoeae MS11

68.696

99.424

0.683
  pilT Neisseria meningitidis 8013

68.696

99.424

0.683
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

68.843

97.118

0.669
  pilT Vibrio cholerae strain A1552

68.843

97.118

0.669
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.304

99.424

0.51
  pilU Acinetobacter baylyi ADP1

42.486

99.712

0.424
  pilU Pseudomonas stutzeri DSM 10701

41.791

96.542

0.403
  pilU Vibrio cholerae strain A1552

40.708

97.695

0.398

Multiple sequence alignment