Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   ACGI6K_RS00635 Genome accession   NZ_CP171449
Coordinates   114577..115611 (+) Length   344 a.a.
NCBI ID   WP_376943052.1    Uniprot ID   -
Organism   Azorhizophilus paspali strain ATCC 23833     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 109577..120611
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACGI6K_RS00600 (ACGI6K_00600) - 109706..110125 (-) 420 WP_376943040.1 DUF4426 domain-containing protein -
  ACGI6K_RS00605 (ACGI6K_00605) metW 110187..110780 (-) 594 WP_376943042.1 methionine biosynthesis protein MetW -
  ACGI6K_RS00610 (ACGI6K_00610) metX 110788..111927 (-) 1140 WP_376943064.1 homoserine O-succinyltransferase MetX -
  ACGI6K_RS00615 (ACGI6K_00615) - 112056..112355 (-) 300 WP_376943044.1 DUF167 domain-containing protein -
  ACGI6K_RS00620 (ACGI6K_00620) - 112355..112948 (-) 594 WP_376943046.1 YggT family protein -
  ACGI6K_RS00625 (ACGI6K_00625) proC 112966..113781 (-) 816 WP_376943048.1 pyrroline-5-carboxylate reductase -
  ACGI6K_RS00630 (ACGI6K_00630) - 113800..114504 (-) 705 WP_376943050.1 YggS family pyridoxal phosphate-dependent enzyme -
  ACGI6K_RS00635 (ACGI6K_00635) pilT 114577..115611 (+) 1035 WP_376943052.1 type IV pilus twitching motility protein PilT Machinery gene
  ACGI6K_RS00640 (ACGI6K_00640) tnpA 115752..116168 (-) 417 WP_376943054.1 IS200/IS605 family transposase -
  ACGI6K_RS00645 (ACGI6K_00645) - 116252..116575 (+) 324 WP_377968946.1 hypothetical protein -
  ACGI6K_RS00650 (ACGI6K_00650) - 116672..117244 (+) 573 WP_377968947.1 RNA-guided endonuclease InsQ/TnpB family protein -
  ACGI6K_RS00655 (ACGI6K_00655) - 117263..117518 (+) 256 Protein_125 type IV pili twitching motility protein PilT -
  ACGI6K_RS00660 (ACGI6K_00660) - 117760..118161 (+) 402 WP_376946387.1 NINE protein -
  ACGI6K_RS00665 (ACGI6K_00665) - 118219..119490 (-) 1272 WP_376946385.1 dihydroorotase -
  ACGI6K_RS00670 (ACGI6K_00670) - 119490..120500 (-) 1011 WP_376946383.1 aspartate carbamoyltransferase catalytic subunit -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 37860.61 Da        Isoelectric Point: 7.1233

>NTDB_id=1061240 ACGI6K_RS00635 WP_376943052.1 114577..115611(+) (pilT) [Azorhizophilus paspali strain ATCC 23833]
MDITELLAFGARQGASDLHLSAGLPPMIRVDGDVRRIDLPPLNHKQVHALIHDIMNDKQRKDYETFFETDFSFEMPGVAR
FRVNAFNQNRGAGAVFRTIPSRVPSMDELGMDEVFRKIAEVPHGLVLVTGPTGSGKSTTLAAMLDHLNGSRHQHILTIED
PIEFVHEPKKCLINQREVHRDTLGFAEALRSVLREDPDIILVGEMRDLETIRLALTAAETGHLVFGTLHTSSAAKTIDRV
VDVFPAEEKAMVRSMLSESLQAVIAQTLLKRVGGGRVAAREIMVGTPAIRNLIREDKVAQMYSAIQTGGSLGMQTLDMAL
KSLLSRGAISRASAREKAKIPDSF

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=1061240 ACGI6K_RS00635 WP_376943052.1 114577..115611(+) (pilT) [Azorhizophilus paspali strain ATCC 23833]
ATGGACATTACCGAACTGCTCGCCTTCGGCGCCAGGCAAGGCGCGTCGGACCTGCACCTTTCCGCCGGCCTGCCGCCGAT
GATCCGCGTCGACGGCGACGTGCGCCGCATCGACCTGCCGCCGCTGAACCACAAGCAGGTGCATGCGCTGATCCACGACA
TCATGAACGACAAGCAGCGCAAGGATTACGAAACGTTTTTCGAAACCGACTTCTCCTTCGAGATGCCAGGCGTGGCGCGC
TTCCGGGTCAACGCCTTCAACCAGAACCGTGGCGCCGGCGCGGTGTTTCGCACCATTCCGTCGCGCGTCCCGAGCATGGA
CGAACTGGGGATGGACGAGGTGTTCAGGAAGATCGCCGAGGTGCCGCACGGCCTGGTGCTGGTCACCGGCCCCACCGGCT
CGGGTAAGTCGACTACCCTGGCGGCCATGCTTGACCACCTGAACGGCAGCAGGCACCAGCACATCCTCACCATCGAGGAC
CCGATCGAGTTCGTCCACGAGCCGAAGAAGTGCCTGATCAACCAGCGCGAGGTGCACCGCGACACCCTGGGCTTCGCCGA
GGCGCTGCGTTCGGTGCTGCGCGAGGACCCGGACATCATCCTGGTCGGCGAGATGCGCGACCTGGAAACCATCCGTTTGG
CGCTGACCGCCGCGGAAACCGGTCACTTGGTGTTCGGCACCCTGCACACCTCCTCGGCGGCCAAGACCATCGACCGGGTG
GTCGATGTGTTCCCGGCGGAAGAGAAGGCGATGGTCCGCTCGATGCTCTCCGAATCGCTGCAGGCGGTGATCGCCCAGAC
CCTGCTGAAGAGGGTCGGTGGCGGCCGGGTGGCGGCCCGCGAAATCATGGTCGGCACCCCGGCGATCCGCAATCTGATCC
GCGAGGACAAGGTGGCGCAGATGTACTCGGCGATCCAGACCGGCGGCTCGCTGGGCATGCAGACCCTGGACATGGCGTTG
AAGAGCCTGCTGTCCAGGGGCGCGATCAGCCGCGCGAGCGCTCGCGAGAAGGCGAAGATTCCCGACAGTTTCTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(12-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

86.047

100

0.86
  pilT Pseudomonas stutzeri DSM 10701

85.756

100

0.858
  pilT Acinetobacter nosocomialis M2

78.488

100

0.785
  pilT Acinetobacter baumannii D1279779

78.198

100

0.782
  pilT Acinetobacter baumannii strain A118

78.198

100

0.782
  pilT Acinetobacter baylyi ADP1

77.616

100

0.776
  pilT Legionella pneumophila strain Lp02

71.893

98.256

0.706
  pilT Legionella pneumophila strain ERS1305867

71.893

98.256

0.706
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

67.647

98.837

0.669
  pilT Vibrio cholerae strain A1552

67.647

98.837

0.669
  pilT Neisseria meningitidis 8013

65.217

100

0.654
  pilT Neisseria gonorrhoeae MS11

64.928

100

0.651
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.884

100

0.52
  pilU Vibrio cholerae strain A1552

41.003

98.547

0.404
  pilU Acinetobacter baylyi ADP1

39.017

100

0.392
  pilU Pseudomonas stutzeri DSM 10701

39.701

97.384

0.387

Multiple sequence alignment