Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   ACGILN_RS17410 Genome accession   NZ_CP171400
Coordinates   3657881..3658903 (+) Length   340 a.a.
NCBI ID   WP_060439204.1    Uniprot ID   -
Organism   Serratia marcescens strain Q20i     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3652881..3663903
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACGILN_RS17375 (ACGILN_17375) - 3653570..3653656 (+) 87 Protein_3405 DUF2442 domain-containing protein -
  ACGILN_RS17380 (ACGILN_17380) hemW 3653693..3654835 (-) 1143 WP_041036677.1 radical SAM family heme chaperone HemW -
  ACGILN_RS17385 (ACGILN_17385) - 3654828..3655421 (-) 594 WP_033635946.1 XTP/dITP diphosphatase -
  ACGILN_RS17390 (ACGILN_17390) yggU 3655439..3655729 (-) 291 WP_394499369.1 DUF167 family protein YggU -
  ACGILN_RS17395 (ACGILN_17395) - 3655726..3656280 (-) 555 WP_033635944.1 YggT family protein -
  ACGILN_RS17400 (ACGILN_17400) proC 3656312..3657133 (-) 822 WP_033635943.1 pyrroline-5-carboxylate reductase -
  ACGILN_RS17405 (ACGILN_17405) - 3657153..3657860 (-) 708 WP_033649587.1 YggS family pyridoxal phosphate-dependent enzyme -
  ACGILN_RS17410 (ACGILN_17410) pilT 3657881..3658903 (+) 1023 WP_060439204.1 type IV pilus twitching motility protein PilT Machinery gene
  ACGILN_RS17415 (ACGILN_17415) ruvX 3658900..3659322 (-) 423 WP_015379046.1 Holliday junction resolvase RuvX -
  ACGILN_RS17420 (ACGILN_17420) - 3659322..3659885 (-) 564 WP_033635940.1 YqgE/AlgH family protein -
  ACGILN_RS17425 (ACGILN_17425) gshB 3660031..3660981 (-) 951 WP_033635939.1 glutathione synthase -
  ACGILN_RS17430 (ACGILN_17430) rsmE 3660992..3661723 (-) 732 WP_033649590.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  ACGILN_RS17435 (ACGILN_17435) endA 3661793..3662488 (-) 696 WP_033649591.1 deoxyribonuclease I -
  ACGILN_RS17440 (ACGILN_17440) - 3662590..3663102 (-) 513 WP_033649592.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 340 a.a.        Molecular weight: 36462.75 Da        Isoelectric Point: 6.8841

>NTDB_id=1061182 ACGILN_RS17410 WP_060439204.1 3657881..3658903(+) (pilT) [Serratia marcescens strain Q20i]
MDIDEFVALSVKHNASDLHLCAGHPPMLRIDGELQPLAAAPTLTAQGVQALCDGLLNAQQRESLRRLGQVDLALHRPGGE
RLRANVFQQSAGMSLALRRIAGQAPSLAELAAPAIVPALLRRDDGLLLVTGATGSGKSTTLAAMIDEINRHQPRHILTLE
DPIEFLHRSRRSLIQQREIGRDSHSFDAALRAALREDPDVILLGELRDIATIRLALTAAETGHLVLATLHTRSAPQAVER
LVDVFPTEEKPYVRAQLAGSLQAVIAQKLMRRPGGGRVAIFEVLTATAAVSNLIREGKTHQLASVLQTGAQSGMQTFEQG
LQQRIDAGVLGECAGEGTEG

Nucleotide


Download         Length: 1023 bp        

>NTDB_id=1061182 ACGILN_RS17410 WP_060439204.1 3657881..3658903(+) (pilT) [Serratia marcescens strain Q20i]
ATGGATATCGATGAATTCGTGGCCCTTAGTGTAAAGCATAATGCTTCCGATCTGCACCTTTGTGCCGGCCATCCGCCGAT
GTTGCGCATCGACGGTGAACTGCAGCCGCTGGCGGCGGCGCCAACGCTGACGGCGCAAGGCGTGCAGGCCCTCTGCGACG
GATTGCTGAATGCGCAGCAGCGCGAGAGCCTGCGGCGGCTGGGGCAGGTCGATCTGGCGCTGCACCGGCCGGGCGGGGAG
CGGCTGCGGGCCAACGTTTTTCAGCAAAGCGCGGGGATGTCTCTCGCATTGCGGCGCATCGCCGGGCAGGCGCCCTCGCT
TGCCGAGCTGGCGGCGCCGGCCATCGTCCCGGCGCTGCTGCGGCGCGACGACGGGCTTCTCCTGGTCACCGGCGCCACCG
GCAGCGGCAAGTCCACCACGCTGGCGGCGATGATCGACGAGATCAACCGGCACCAGCCGCGGCACATTTTGACGCTGGAG
GATCCGATCGAATTCCTGCACCGCAGCCGGCGTTCGCTGATCCAGCAGCGGGAGATCGGCCGCGACAGCCACAGCTTCGA
TGCGGCGTTGCGCGCCGCGCTGCGCGAAGATCCGGATGTGATCCTGCTGGGTGAGCTGCGCGATATCGCCACCATCCGGC
TGGCGCTCACCGCGGCGGAGACCGGCCATCTGGTGCTGGCGACGTTGCACACCCGCAGCGCGCCGCAGGCGGTGGAACGG
CTGGTGGACGTGTTTCCGACGGAAGAGAAACCCTATGTGCGCGCCCAGCTGGCCGGCAGCCTGCAGGCGGTGATCGCGCA
AAAGCTGATGAGGCGGCCCGGCGGTGGGCGAGTGGCGATCTTTGAGGTGCTGACGGCGACGGCGGCGGTCAGCAACCTGA
TCCGCGAAGGGAAAACCCACCAGTTGGCGAGCGTGTTGCAAACCGGCGCGCAGTCCGGCATGCAAACCTTTGAGCAAGGT
TTGCAGCAGCGGATCGACGCCGGCGTGTTGGGGGAGTGTGCGGGGGAGGGAACGGAGGGTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(13-272)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

54.848

97.059

0.532
  pilT Vibrio cholerae strain A1552

54.848

97.059

0.532
  pilT Acinetobacter baylyi ADP1

51.057

97.353

0.497
  pilT Acinetobacter nosocomialis M2

50.606

97.059

0.491
  pilT Acinetobacter baumannii D1279779

50.606

97.059

0.491
  pilT Acinetobacter baumannii strain A118

50.606

97.059

0.491
  pilT Neisseria meningitidis 8013

50.301

97.647

0.491
  pilT Neisseria gonorrhoeae MS11

50

97.647

0.488
  pilT Pseudomonas aeruginosa PAK

49.394

97.059

0.479
  pilT Legionella pneumophila strain ERS1305867

49.096

97.647

0.479
  pilT Legionella pneumophila strain Lp02

49.096

97.647

0.479
  pilT Pseudomonas stutzeri DSM 10701

48.788

97.059

0.474
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.82

100

0.459
  pilU Vibrio cholerae strain A1552

39.877

95.882

0.382
  pilU Acinetobacter baylyi ADP1

39.628

95

0.376
  pilU Pseudomonas stutzeri DSM 10701

38.58

95.294

0.368

Multiple sequence alignment