Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   ACF8MK_RS16800 Genome accession   NZ_CP171154
Coordinates   3884133..3885167 (-) Length   344 a.a.
NCBI ID   WP_265702880.1    Uniprot ID   -
Organism   Legionella sp. 227     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3879133..3890167
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACF8MK_RS16765 (ACF8MK_16765) rpoZ 3879500..3879703 (-) 204 WP_010653170.1 DNA-directed RNA polymerase subunit omega -
  ACF8MK_RS16770 (ACF8MK_16770) gmk 3879735..3880364 (-) 630 WP_392538982.1 guanylate kinase -
  ACF8MK_RS16775 (ACF8MK_16775) - 3880370..3881236 (-) 867 WP_392537620.1 YicC/YloC family endoribonuclease -
  ACF8MK_RS16780 (ACF8MK_16780) - 3881501..3881887 (+) 387 WP_392537621.1 hypothetical protein -
  ACF8MK_RS16785 (ACF8MK_16785) - 3881911..3882777 (-) 867 WP_392537622.1 MC/SLC25 family protein -
  ACF8MK_RS16790 (ACF8MK_16790) - 3882862..3882996 (-) 135 WP_392537623.1 hypothetical protein -
  ACF8MK_RS16795 (ACF8MK_16795) rph 3883262..3883969 (+) 708 WP_392537624.1 ribonuclease PH -
  ACF8MK_RS16800 (ACF8MK_16800) pilT 3884133..3885167 (-) 1035 WP_265702880.1 type IV pilus twitching motility protein PilT Machinery gene
  ACF8MK_RS16805 (ACF8MK_16805) - 3885407..3886093 (+) 687 WP_392538983.1 YggS family pyridoxal phosphate-dependent enzyme -
  ACF8MK_RS16810 (ACF8MK_16810) - 3886141..3886716 (+) 576 WP_392537625.1 YggT family protein -
  ACF8MK_RS16815 (ACF8MK_16815) - 3886763..3887329 (+) 567 WP_392537626.1 DUF2845 domain-containing protein -
  ACF8MK_RS16820 (ACF8MK_16820) - 3887466..3887747 (+) 282 WP_392537627.1 hypothetical protein -
  ACF8MK_RS16825 (ACF8MK_16825) - 3888054..3889589 (+) 1536 WP_392537628.1 hypothetical protein -
  ACF8MK_RS16830 (ACF8MK_16830) - 3889722..3889955 (+) 234 WP_392537629.1 hypothetical protein -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 38303.16 Da        Isoelectric Point: 6.6653

>NTDB_id=1060354 ACF8MK_RS16800 WP_265702880.1 3884133..3885167(-) (pilT) [Legionella sp. 227]
MDIAELLAFSVKNKSSDLHISAGLPPLIRVDGDLRKINLPAMEHKEVIKIIYDVMNDRQRKEFEESLETDFSFEIPNLAR
FRVNVFHQLRGAAAVFRTIPSEILSMSDLGLPSIFEEMSSYPKGLVLITGPTGSGKSTTLAAMIDYINSSRYDHILTVED
PIEFVHQSKKCLLNQREVHRDTLSFNAALRSALREDPDVILVGELRDLETIRLAMTAAETGHLVFGTLHTNSATKTINRI
IDVFPAEEKSMVRSMLSESLQAVVAQTLLKKNGGGRVAALEIMICTGAIRNLIREDKVAQMYSSIQTGQGKGMQTLDQHL
TELVKKNIISTHTARDVAIDKNLF

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=1060354 ACF8MK_RS16800 WP_265702880.1 3884133..3885167(-) (pilT) [Legionella sp. 227]
ATGGATATAGCAGAATTATTAGCGTTTTCAGTAAAAAATAAATCTTCGGACTTACATATCTCTGCGGGATTGCCGCCCTT
AATTCGTGTGGATGGGGATTTACGTAAAATTAATTTGCCAGCCATGGAACACAAGGAAGTAATAAAAATTATCTATGATG
TGATGAATGATAGACAAAGGAAGGAGTTTGAAGAGAGTTTGGAAACCGATTTTTCTTTTGAAATACCCAATTTGGCTCGC
TTTAGGGTGAACGTATTTCATCAATTACGTGGTGCAGCAGCTGTTTTTCGTACTATTCCTTCTGAAATTTTAAGTATGAG
CGATTTGGGTTTACCCTCTATTTTCGAGGAAATGTCCAGTTACCCTAAAGGATTAGTTCTTATTACTGGTCCAACAGGGT
CAGGAAAAAGTACTACCCTGGCCGCCATGATCGATTACATTAATTCCAGTCGTTATGATCATATTTTAACTGTTGAGGAT
CCAATAGAATTTGTACATCAAAGTAAAAAGTGCTTGCTAAATCAGCGCGAGGTTCATCGAGATACACTGAGTTTTAACGC
TGCTTTGCGTTCTGCACTGCGTGAAGATCCTGATGTGATTCTGGTGGGGGAGTTGCGTGATTTGGAAACGATCCGTTTAG
CCATGACTGCTGCTGAGACTGGACACTTGGTGTTTGGCACCTTGCATACTAATTCGGCAACTAAAACCATTAACCGGATT
ATCGATGTGTTTCCCGCCGAAGAAAAATCCATGGTACGTTCGATGTTGTCTGAGTCATTGCAAGCTGTAGTTGCACAAAC
GCTATTGAAAAAAAATGGGGGAGGGCGAGTTGCTGCGTTGGAAATCATGATTTGCACTGGCGCAATACGAAACTTAATTC
GCGAAGATAAAGTGGCTCAAATGTATTCCTCCATCCAAACAGGCCAAGGTAAAGGAATGCAAACTTTGGATCAGCATTTA
ACAGAGTTGGTGAAAAAAAATATTATTTCCACTCATACGGCACGTGATGTGGCTATTGATAAAAACCTATTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Legionella pneumophila strain ERS1305867

88.663

100

0.887
  pilT Legionella pneumophila strain Lp02

88.663

100

0.887
  pilT Acinetobacter baylyi ADP1

76.923

98.256

0.756
  pilT Acinetobacter baumannii strain A118

76.331

98.256

0.75
  pilT Acinetobacter nosocomialis M2

76.331

98.256

0.75
  pilT Acinetobacter baumannii D1279779

76.331

98.256

0.75
  pilT Pseudomonas stutzeri DSM 10701

74.852

98.256

0.735
  pilT Pseudomonas aeruginosa PAK

74.26

98.256

0.73
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

68.085

95.64

0.651
  pilT Vibrio cholerae strain A1552

68.085

95.64

0.651
  pilT Neisseria meningitidis 8013

65.476

97.674

0.64
  pilT Neisseria gonorrhoeae MS11

65.179

97.674

0.637
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

48.986

100

0.491
  pilU Vibrio cholerae strain A1552

42.09

97.384

0.41
  pilU Pseudomonas stutzeri DSM 10701

41.791

97.384

0.407
  pilU Acinetobacter baylyi ADP1

39.157

96.512

0.378

Multiple sequence alignment