Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   ACF3M1_RS14045 Genome accession   NZ_CP171131
Coordinates   3105592..3106704 (+) Length   370 a.a.
NCBI ID   WP_394001661.1    Uniprot ID   -
Organism   Luteimonas sp. WGS1318     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3100592..3111704
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACF3M1_RS14020 (ACF3M1_14020) - 3101144..3102328 (-) 1185 WP_394001653.1 cystathionine gamma-synthase -
  ACF3M1_RS14025 (ACF3M1_14025) - 3102408..3103784 (-) 1377 WP_394001655.1 pyridoxal-phosphate dependent enzyme -
  ACF3M1_RS14030 (ACF3M1_14030) - 3103925..3104143 (-) 219 WP_096209859.1 YdcH family protein -
  ACF3M1_RS14035 (ACF3M1_14035) - 3104545..3105117 (-) 573 WP_394001657.1 hypothetical protein -
  ACF3M1_RS14040 (ACF3M1_14040) - 3105120..3105500 (-) 381 WP_394001659.1 DUF4398 domain-containing protein -
  ACF3M1_RS14045 (ACF3M1_14045) pilU 3105592..3106704 (+) 1113 WP_394001661.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ACF3M1_RS14050 (ACF3M1_14050) maiA 3106730..3107386 (-) 657 WP_394001662.1 maleylacetoacetate isomerase -
  ACF3M1_RS14055 (ACF3M1_14055) - 3107392..3108384 (-) 993 WP_394001665.1 fumarylacetoacetate hydrolase family protein -
  ACF3M1_RS14060 (ACF3M1_14060) - 3108475..3109209 (-) 735 WP_394001667.1 2OG-Fe dioxygenase family protein -
  ACF3M1_RS14070 (ACF3M1_14070) rpsI 3109482..3109874 (-) 393 WP_394001669.1 30S ribosomal protein S9 -
  ACF3M1_RS14075 (ACF3M1_14075) rplM 3109878..3110306 (-) 429 WP_096209866.1 50S ribosomal protein L13 -
  ACF3M1_RS14080 (ACF3M1_14080) coq7 3110528..3111175 (+) 648 WP_394001672.1 2-polyprenyl-3-methyl-6-methoxy-1,4-benzoquinone monooxygenase -

Sequence

Protein


Download         Length: 370 a.a.        Molecular weight: 41230.41 Da        Isoelectric Point: 6.2885

>NTDB_id=1060285 ACF3M1_RS14045 WP_394001661.1 3105592..3106704(+) (pilU) [Luteimonas sp. WGS1318]
MDIGYFLKLMTEKNASDMFLSTGAPVYIKVEGRLYPLGSTGLPPGMVKKIAYSLMDENQIQQFERDMEMNMAYSLQDAGR
FRINVFRQRGEAGMVVRAIRSTIPSIEELQLPQVLKDIIMAPRGLVLIVGSTGSGKSTTLASMIDHRNASTSGHILTIED
PIEYLHRHKKSLVNQREVGLDTQTFHNALKNAMREAPDVILIGEILDATTMEAAIAFAETGHICLATLHSNNADQTIERI
LNFFPEAAHKNVLMNLALNLRAVVSQRLVQGMDGRRMPATEVLLNTPMIRDLLRRGQVHEIKQAMEESLEDGMETFDQCL
FRLYKEGRIERDTALRAADSRDGLDLKFRLSEGGMGAHDPYADVFAAGGH

Nucleotide


Download         Length: 1113 bp        

>NTDB_id=1060285 ACF3M1_RS14045 WP_394001661.1 3105592..3106704(+) (pilU) [Luteimonas sp. WGS1318]
ATGGATATCGGCTACTTCCTGAAGCTGATGACGGAAAAGAACGCTTCGGACATGTTCCTCAGCACCGGCGCGCCGGTGTA
CATCAAAGTCGAGGGACGGCTGTATCCGCTGGGCAGCACCGGCCTGCCGCCGGGCATGGTCAAGAAGATCGCGTACTCGC
TGATGGACGAGAACCAGATCCAGCAGTTCGAGCGCGACATGGAAATGAACATGGCGTACTCGCTGCAGGACGCCGGCCGC
TTCCGTATCAACGTGTTCCGCCAGCGCGGCGAAGCCGGCATGGTGGTGCGTGCGATCCGCAGCACCATTCCCAGCATCGA
GGAGCTGCAGCTGCCGCAGGTGCTCAAGGACATCATCATGGCGCCGCGCGGCCTGGTGCTGATCGTGGGCTCCACTGGCT
CGGGCAAGTCGACCACGCTGGCTTCGATGATCGACCACCGCAATGCCAGCACCTCGGGCCACATCCTCACCATCGAGGAT
CCGATCGAGTACCTGCACCGGCACAAGAAGTCGCTGGTCAACCAGCGCGAGGTCGGCCTGGACACGCAGACCTTCCACAA
CGCGCTCAAGAACGCCATGCGCGAGGCGCCGGACGTCATCCTGATCGGTGAAATCCTCGACGCGACCACGATGGAAGCGG
CGATCGCGTTCGCCGAAACCGGTCACATCTGCCTGGCGACGCTGCACTCGAACAATGCCGACCAGACCATCGAGCGCATC
CTCAACTTCTTCCCCGAGGCCGCGCACAAGAACGTGCTGATGAACCTGGCGCTGAATCTGCGCGCAGTGGTGTCGCAGCG
TCTGGTCCAGGGCATGGACGGCCGCCGCATGCCGGCGACCGAGGTGCTGCTCAACACGCCGATGATCCGCGACCTGCTGC
GCCGCGGCCAGGTGCACGAGATCAAGCAGGCGATGGAAGAATCGCTGGAAGACGGCATGGAGACCTTCGACCAGTGCCTG
TTCCGCCTCTACAAGGAAGGCCGTATCGAACGCGACACCGCGCTGCGCGCGGCCGATTCGCGCGACGGGCTGGACCTCAA
GTTCCGGCTGTCGGAAGGCGGCATGGGCGCGCACGATCCGTACGCGGACGTGTTCGCCGCGGGCGGTCACTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(69-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

50.139

97.027

0.486
  pilU Acinetobacter baylyi ADP1

47.458

95.676

0.454
  pilU Vibrio cholerae strain A1552

47.175

95.676

0.451
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

45.404

97.027

0.441
  pilT Acinetobacter baylyi ADP1

41.471

91.892

0.381
  pilT Pseudomonas aeruginosa PAK

41.716

91.351

0.381
  pilT Legionella pneumophila strain ERS1305867

41.716

91.351

0.381
  pilT Legionella pneumophila strain Lp02

41.716

91.351

0.381
  pilT Acinetobacter baumannii D1279779

41.42

91.351

0.378
  pilT Pseudomonas stutzeri DSM 10701

41.42

91.351

0.378
  pilT Acinetobacter nosocomialis M2

41.42

91.351

0.378
  pilT Acinetobacter baumannii strain A118

41.42

91.351

0.378
  pilT Neisseria meningitidis 8013

39.368

94.054

0.37
  pilT Neisseria gonorrhoeae MS11

39.368

94.054

0.37
  pilT Vibrio cholerae strain A1552

39.481

93.784

0.37
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

39.481

93.784

0.37

Multiple sequence alignment