Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   ACFS0M_RS04145 Genome accession   NZ_CP170911
Coordinates   814350..815330 (+) Length   326 a.a.
NCBI ID   WP_002916609.1    Uniprot ID   W8UCM3
Organism   Klebsiella pneumoniae strain 2016C06-059     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 809350..820330
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACFS0M_RS04110 (ACFS0M_04110) - 809886..810212 (+) 327 WP_002916620.1 YggL family protein -
  ACFS0M_RS04115 (ACFS0M_04115) - 810263..810982 (+) 720 WP_002916619.1 DUF2884 domain-containing protein -
  ACFS0M_RS04120 (ACFS0M_04120) hemW 811023..812159 (-) 1137 WP_002916617.1 radical SAM family heme chaperone HemW -
  ACFS0M_RS04125 (ACFS0M_04125) - 812152..812745 (-) 594 WP_002916615.1 XTP/dITP diphosphatase -
  ACFS0M_RS04130 (ACFS0M_04130) yggU 812758..813048 (-) 291 WP_002916614.1 DUF167 family protein YggU -
  ACFS0M_RS04135 (ACFS0M_04135) - 813045..813611 (-) 567 WP_002916613.1 YggT family protein -
  ACFS0M_RS04140 (ACFS0M_04140) - 813632..814333 (-) 702 WP_002916611.1 YggS family pyridoxal phosphate-dependent enzyme -
  ACFS0M_RS04145 (ACFS0M_04145) pilT 814350..815330 (+) 981 WP_002916609.1 type IV pilus twitching motility protein PilT Machinery gene
  ACFS0M_RS04150 (ACFS0M_04150) - 815679..816332 (+) 654 WP_002916607.1 LuxR family transcriptional regulator -
  ACFS0M_RS04155 (ACFS0M_04155) ruvX 816385..816801 (-) 417 WP_002916605.1 Holliday junction resolvase RuvX -
  ACFS0M_RS04160 (ACFS0M_04160) - 816801..817364 (-) 564 WP_002916603.1 YqgE/AlgH family protein -
  ACFS0M_RS04165 (ACFS0M_04165) gshB 817539..818489 (-) 951 WP_002916600.1 glutathione synthase -
  ACFS0M_RS04170 (ACFS0M_04170) rsmE 818510..819241 (-) 732 WP_002916597.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  ACFS0M_RS04175 (ACFS0M_04175) endA 819333..820040 (-) 708 WP_002916589.1 deoxyribonuclease I -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35230.71 Da        Isoelectric Point: 6.8544

>NTDB_id=1059659 ACFS0M_RS04145 WP_002916609.1 814350..815330(+) (pilT) [Klebsiella pneumoniae strain 2016C06-059]
MKLEEIVALSVKHNVSDLHLCNSAAPRWRRQGRLQPAPFPAPDIANLLNDWLDAAQLLHWQEHGQIDFALTLACGARLRA
SAFAHTRGISLVLRLLPEQCPRLDTLGAPPALSELLAEESGLLLVTGATGSGKSTTLAAMVGHLNQHLDGHILTLEDPVE
FIHHSERCLIQQREVGRHCPSFAAALRVALRQDPDVILLGELRDSETIRLALTAAETGHLVMATLHTRGAAPAVERLIDV
FPAEEKDQVRSQLAGSLCAVLAQKLLPARQGGRVALYELLVNTPAVANLIREGKVHQLPGVMQTGMQAGMLTFTQSFQQC
VAAGAL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=1059659 ACFS0M_RS04145 WP_002916609.1 814350..815330(+) (pilT) [Klebsiella pneumoniae strain 2016C06-059]
ATGAAGCTGGAAGAAATCGTAGCCCTTAGTGTAAAGCATAATGTCTCCGATCTACACCTGTGCAATTCCGCCGCACCACG
CTGGCGGCGGCAGGGCAGGCTGCAGCCCGCGCCCTTTCCCGCGCCGGATATTGCGAATTTACTCAACGACTGGCTCGATG
CCGCACAGTTACTGCACTGGCAGGAACACGGCCAGATTGACTTTGCGCTGACCCTGGCCTGCGGTGCGCGGCTGCGCGCC
AGCGCCTTCGCCCATACGCGTGGGATATCGCTGGTGCTGCGCCTCCTTCCTGAACAGTGCCCGCGTCTGGACACGCTCGG
CGCCCCGCCTGCGCTGAGCGAACTGCTGGCGGAAGAGAGTGGTTTGCTGTTGGTCACCGGAGCGACGGGCAGCGGCAAGT
CGACCACCCTGGCGGCAATGGTGGGGCACCTCAATCAGCATCTTGATGGCCATATTCTGACGCTTGAAGATCCGGTGGAA
TTTATTCATCACAGCGAGCGCTGTCTGATCCAGCAGCGGGAAGTTGGCCGTCACTGTCCTTCGTTCGCCGCGGCGCTCCG
CGTGGCGCTGCGCCAGGATCCGGATGTGATCCTGCTCGGGGAGCTACGCGATAGCGAAACCATTCGCCTGGCGTTAACCG
CGGCGGAGACCGGGCACCTGGTAATGGCGACATTACATACCCGCGGCGCGGCGCCGGCGGTGGAGAGATTAATCGATGTC
TTTCCGGCGGAGGAGAAAGATCAGGTTCGTAGCCAGCTTGCCGGAAGCCTGTGCGCGGTACTGGCGCAAAAATTGTTACC
TGCACGTCAGGGCGGGAGAGTTGCGCTATACGAGCTTCTGGTCAACACGCCAGCGGTGGCGAATTTGATCCGTGAAGGAA
AAGTACATCAATTGCCTGGTGTGATGCAGACCGGGATGCAGGCCGGAATGTTGACCTTTACCCAGAGTTTTCAGCAATGT
GTCGCTGCAGGCGCGCTATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-266)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB W8UCM3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

51.368

100

0.518
  pilT Vibrio cholerae strain A1552

51.368

100

0.518
  pilT Acinetobacter baylyi ADP1

48.936

100

0.494
  pilT Neisseria meningitidis 8013

48.171

100

0.485
  pilT Acinetobacter baumannii D1279779

48.024

100

0.485
  pilT Acinetobacter baumannii strain A118

48.024

100

0.485
  pilT Neisseria gonorrhoeae MS11

47.866

100

0.482
  pilT Acinetobacter nosocomialis M2

47.72

100

0.482
  pilT Legionella pneumophila strain ERS1305867

48.148

99.387

0.479
  pilT Legionella pneumophila strain Lp02

48.148

99.387

0.479
  pilT Pseudomonas stutzeri DSM 10701

47.401

100

0.475
  pilT Pseudomonas aeruginosa PAK

46.789

100

0.469
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.795

97.239

0.436
  pilU Vibrio cholerae strain A1552

41.358

99.387

0.411
  pilU Pseudomonas stutzeri DSM 10701

39.394

100

0.399
  pilU Acinetobacter baylyi ADP1

39.875

98.466

0.393

Multiple sequence alignment