Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   ACF3DV_RS13185 Genome accession   NZ_CP170743
Coordinates   3052828..3053955 (+) Length   375 a.a.
NCBI ID   WP_016874201.1    Uniprot ID   -
Organism   Chlorogloeopsis fritschii PCC 9212     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3047828..3058955
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACF3DV_RS13175 (ACF3DV_13175) grpE 3049392..3050129 (-) 738 WP_016874199.1 nucleotide exchange factor GrpE -
  ACF3DV_RS13180 (ACF3DV_13180) pilB/pilB1 3050743..3052755 (+) 2013 WP_016874200.1 GspE/PulE family protein Machinery gene
  ACF3DV_RS13185 (ACF3DV_13185) pilT 3052828..3053955 (+) 1128 WP_016874201.1 type IV pilus twitching motility protein PilT Machinery gene
  ACF3DV_RS13190 (ACF3DV_13190) - 3054078..3055292 (+) 1215 WP_016874202.1 type II secretion system F family protein -
  ACF3DV_RS13195 (ACF3DV_13195) - 3055361..3055921 (+) 561 WP_016874203.1 hypothetical protein -
  ACF3DV_RS13200 (ACF3DV_13200) - 3056102..3056461 (+) 360 WP_016874204.1 hypothetical protein -
  ACF3DV_RS13205 (ACF3DV_13205) - 3056479..3058416 (+) 1938 WP_016874205.1 hypothetical protein -

Sequence

Protein


Download         Length: 375 a.a.        Molecular weight: 41006.32 Da        Isoelectric Point: 7.0454

>NTDB_id=1059111 ACF3DV_RS13185 WP_016874201.1 3052828..3053955(+) (pilT) [Chlorogloeopsis fritschii PCC 9212]
MEMMIEDLMEQMIEMGGSDMHLSAGLPPYFRISGKLTPIGEEPLSGDQCQRLIFSMLNNSQRKTLEQNWELDCSYGVKGL
ARFRVNVYKERGTYAACLRALSSKIPNFDKLGLPDVVREMADKPRGLILVTGPTGSGKTTTLAAIIDLINRTRAEHILTI
EDPIEFVYEPIKSLVHQRQLNEDTKSFANALRAALREDPDIVLIGEMRDLETISLAISAAETGHLVFGTLHTSSAAQTVD
RIIDVFPADRQTQIRVQLSNSLVAVFSQTLISKKNPKPGEFGRVMAQEIMIVTPAISNLIREGKTAQIYSAIQTGGKLGM
QTLEKVLADLYKQGTISFEAAIAKTSKPDEVQRLIGSAPPSAAGKPGVAATVKAH

Nucleotide


Download         Length: 1128 bp        

>NTDB_id=1059111 ACF3DV_RS13185 WP_016874201.1 3052828..3053955(+) (pilT) [Chlorogloeopsis fritschii PCC 9212]
ATGGAAATGATGATTGAAGATTTAATGGAGCAGATGATTGAAATGGGAGGTTCGGATATGCATTTATCCGCTGGCTTACC
TCCTTATTTTCGTATTAGTGGCAAACTCACTCCCATTGGTGAAGAACCATTGTCTGGGGATCAATGCCAAAGGCTGATTT
TTAGTATGTTAAATAACTCCCAAAGGAAGACATTAGAGCAGAACTGGGAGTTAGATTGCTCCTATGGTGTGAAGGGATTA
GCACGTTTTCGCGTCAATGTTTACAAAGAGCGCGGTACCTACGCGGCTTGCTTGCGGGCGTTAAGTTCTAAAATTCCCAA
CTTTGATAAATTAGGTTTGCCAGATGTAGTGCGGGAAATGGCAGACAAACCCAGAGGATTAATTTTGGTTACAGGGCCTA
CGGGTTCTGGAAAGACAACTACCCTAGCAGCAATTATCGATTTAATTAACCGCACGCGAGCAGAACATATCTTAACAATA
GAAGATCCAATTGAGTTTGTTTACGAACCAATTAAAAGTCTTGTACACCAACGGCAGTTGAATGAAGATACCAAGAGTTT
TGCCAATGCTTTGAGAGCTGCTTTACGAGAAGATCCAGACATTGTCCTAATTGGGGAAATGCGAGATTTAGAAACTATTT
CTCTGGCAATTTCCGCTGCTGAAACTGGACATTTAGTATTTGGAACTTTGCACACTAGTTCAGCCGCGCAAACTGTTGAC
AGAATCATTGACGTTTTCCCTGCGGATAGACAAACTCAGATCCGGGTGCAGTTATCTAATTCACTTGTAGCCGTATTTAG
TCAAACTTTAATCTCCAAGAAAAACCCCAAACCAGGTGAGTTTGGTAGGGTAATGGCACAGGAGATTATGATAGTTACTC
CTGCTATTTCTAACTTGATTCGTGAAGGGAAAACCGCCCAAATTTATTCAGCTATTCAAACAGGCGGTAAGTTGGGAATG
CAAACCTTAGAAAAAGTTCTAGCCGATTTGTACAAGCAGGGAACTATCTCATTTGAAGCAGCAATAGCTAAAACTTCTAA
GCCAGATGAAGTACAACGTCTGATTGGTTCTGCCCCACCATCTGCTGCTGGAAAACCTGGTGTTGCTGCTACTGTTAAAG
CACACTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(45-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

55.331

92.533

0.512
  pilT Pseudomonas stutzeri DSM 10701

54.179

92.533

0.501
  pilT Legionella pneumophila strain ERS1305867

53.314

92.533

0.493
  pilT Legionella pneumophila strain Lp02

53.314

92.533

0.493
  pilT Acinetobacter baylyi ADP1

52.738

92.533

0.488
  pilT Acinetobacter baumannii strain A118

51.585

92.533

0.477
  pilT Acinetobacter baumannii D1279779

51.585

92.533

0.477
  pilT Acinetobacter nosocomialis M2

51.585

92.533

0.477
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

48.108

98.667

0.475
  pilT Neisseria gonorrhoeae MS11

50.442

90.4

0.456
  pilT Neisseria meningitidis 8013

50.442

90.4

0.456
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50

89.067

0.445
  pilT Vibrio cholerae strain A1552

50

89.067

0.445
  pilU Acinetobacter baylyi ADP1

39.377

94.133

0.371
  pilU Pseudomonas stutzeri DSM 10701

39.093

94.133

0.368

Multiple sequence alignment