Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB/pilB1   Type   Machinery gene
Locus tag   ACF3DV_RS13180 Genome accession   NZ_CP170743
Coordinates   3050743..3052755 (+) Length   670 a.a.
NCBI ID   WP_016874200.1    Uniprot ID   A0A3S0Y2P4
Organism   Chlorogloeopsis fritschii PCC 9212     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3045743..3057755
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACF3DV_RS13170 (ACF3DV_13170) dnaK 3047168..3049120 (-) 1953 WP_016874198.1 molecular chaperone DnaK -
  ACF3DV_RS13175 (ACF3DV_13175) grpE 3049392..3050129 (-) 738 WP_016874199.1 nucleotide exchange factor GrpE -
  ACF3DV_RS13180 (ACF3DV_13180) pilB/pilB1 3050743..3052755 (+) 2013 WP_016874200.1 GspE/PulE family protein Machinery gene
  ACF3DV_RS13185 (ACF3DV_13185) pilT 3052828..3053955 (+) 1128 WP_016874201.1 type IV pilus twitching motility protein PilT Machinery gene
  ACF3DV_RS13190 (ACF3DV_13190) - 3054078..3055292 (+) 1215 WP_016874202.1 type II secretion system F family protein -
  ACF3DV_RS13195 (ACF3DV_13195) - 3055361..3055921 (+) 561 WP_016874203.1 hypothetical protein -
  ACF3DV_RS13200 (ACF3DV_13200) - 3056102..3056461 (+) 360 WP_016874204.1 hypothetical protein -

Sequence

Protein


Download         Length: 670 a.a.        Molecular weight: 74646.32 Da        Isoelectric Point: 5.1110

>NTDB_id=1059110 ACF3DV_RS13180 WP_016874200.1 3050743..3052755(+) (pilB/pilB1) [Chlorogloeopsis fritschii PCC 9212]
MTYSSPQRRSTALTTRTEFSPFGNKLVASGFVDSEQMKQALMESRKSGRPLTEVLESISGRQLSPELLRQHKKQQLFELK
ILYGVESLDPEVSPVGNTTIANLIDALIPADICRRHRLVPLAKNEDQNPPSVLVAMVDPDNLEASDDLNRILRPQGLVLQ
RMVIAQEDYQQLINQYLDDLAIRQKHLEQEKYTDINQDLENLENLDVNDAPEEADADLGAAMKGAEDAPVINLVNRILAK
ALHEKVSDIHVEPQEENLRIRFRKDGVLREAFDPLPKKIIPAVTARFKIISNLDIAERRLPQDGRIRRLFEGRKVDFRVN
TLPSRYGEKVCLRILDNSATQLGLDKLITDPETLQIVQEMVSRPFGLILVTGPTGSGKTTSLYSALAEKNSPGINISTVE
DPIEYSLPGITQVQVIREKGLDFATALRAFLRQDPDVLLVGETRDKETAKTAIEAALTGHLVLTTLHTNDAPGAIARLGE
MGIEPFMVSSSLIGVLAQRLMRRVCPDCRIPYTPTVDELARYGLSASQEVGVTFYKANTLTGEQLQEAKAKGHLCSTCNG
VGYKGRSGVYEVMRITENLQTLINEEAPTERIKEVAVEEGMKTLLAYSLDLVRQGTTTLEEVERVTFTDTGLEAELKAKR
KTSLTCRTCNAGLQPEWMDCPYCMTPRFQD

Nucleotide


Download         Length: 2013 bp        

>NTDB_id=1059110 ACF3DV_RS13180 WP_016874200.1 3050743..3052755(+) (pilB/pilB1) [Chlorogloeopsis fritschii PCC 9212]
ATGACTTACTCGTCACCACAACGGCGCAGTACCGCTCTCACTACCAGAACAGAGTTTTCACCCTTTGGCAATAAACTTGT
CGCATCTGGCTTCGTCGATAGCGAACAGATGAAACAAGCGTTAATGGAAAGTCGCAAGTCTGGTAGACCGTTGACAGAGG
TACTGGAATCGATATCAGGGCGACAACTATCACCAGAGTTACTCAGACAGCACAAAAAGCAGCAATTATTTGAACTTAAA
ATACTTTACGGTGTTGAATCCCTCGATCCGGAAGTTAGTCCTGTAGGTAACACAACGATCGCCAATCTCATTGATGCTCT
CATTCCAGCAGATATCTGTCGCCGTCATCGCTTAGTACCACTGGCAAAAAATGAAGACCAAAACCCACCTTCAGTTTTGG
TGGCGATGGTCGATCCAGATAATCTAGAAGCATCAGACGATCTCAATCGCATCTTGCGCCCCCAAGGCTTAGTCTTACAG
CGGATGGTAATTGCTCAGGAAGATTACCAACAGCTAATCAACCAATACTTGGATGATTTGGCTATTCGGCAAAAGCACTT
GGAACAAGAAAAATATACAGACATTAATCAGGATTTAGAAAATCTAGAAAATCTAGATGTCAATGATGCTCCTGAAGAAG
CTGATGCTGATTTGGGAGCGGCAATGAAAGGTGCCGAAGATGCCCCGGTGATTAATTTGGTCAATAGAATCCTGGCAAAA
GCACTGCATGAAAAGGTTTCTGATATTCACGTCGAACCACAAGAAGAAAACTTACGTATTCGCTTTCGCAAGGATGGAGT
ATTGCGCGAGGCATTCGATCCTCTGCCCAAAAAAATCATTCCGGCTGTGACAGCTCGATTTAAGATTATCTCCAACTTAG
ATATTGCCGAACGGCGTCTACCCCAAGATGGACGTATCCGCCGTCTGTTTGAAGGACGTAAAGTAGACTTTCGTGTCAAT
ACCTTACCTAGTCGCTACGGCGAAAAAGTTTGTCTGCGGATTTTAGATAATTCTGCAACGCAACTTGGTTTGGATAAATT
AATTACCGATCCAGAGACATTGCAAATTGTCCAAGAAATGGTTAGCCGTCCCTTTGGACTAATTTTAGTCACAGGGCCGA
CCGGATCTGGTAAAACAACTTCTTTGTATTCGGCACTAGCAGAAAAAAATAGCCCTGGAATTAATATCAGTACCGTTGAA
GATCCCATCGAATACAGCTTGCCGGGGATTACTCAGGTGCAGGTAATTCGGGAAAAAGGATTAGATTTTGCCACTGCATT
GCGAGCTTTCTTGCGGCAAGATCCGGATGTGCTGCTGGTGGGTGAAACGCGGGATAAAGAAACAGCAAAAACAGCGATTG
AAGCAGCATTAACAGGTCACTTAGTATTAACTACCTTACACACCAATGATGCTCCTGGTGCGATCGCCCGTTTAGGAGAA
ATGGGCATTGAACCTTTCATGGTTTCCAGTTCGCTAATCGGTGTTTTAGCACAACGTTTGATGCGCCGTGTGTGCCCCGA
TTGTCGCATTCCTTATACTCCCACAGTAGATGAACTGGCTCGCTATGGTTTATCAGCCTCCCAAGAAGTGGGAGTCACCT
TCTACAAAGCTAACACTCTAACTGGAGAGCAACTGCAAGAAGCAAAAGCCAAAGGGCATCTTTGCTCTACTTGTAACGGT
GTTGGCTACAAAGGACGTAGTGGTGTCTACGAGGTAATGCGAATTACTGAAAACCTGCAAACCCTCATTAATGAAGAAGC
ACCAACAGAACGCATCAAAGAAGTAGCAGTAGAAGAAGGAATGAAAACTTTGCTGGCGTACAGTTTAGACTTAGTGCGCC
AAGGCACAACCACCTTAGAAGAAGTAGAACGGGTAACTTTCACCGATACAGGTTTAGAAGCTGAGTTGAAAGCCAAACGC
AAGACAAGCCTTACCTGCCGGACTTGTAATGCTGGATTGCAACCAGAGTGGATGGATTGTCCTTACTGTATGACGCCTCG
ATTTCAAGATTAG

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(27-179)

Type II/IV secretion system protein (T2SSE)

(236-503)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A3S0Y2P4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB/pilB1 Synechocystis sp. PCC 6803

66.964

100

0.672

Multiple sequence alignment